Team:Evry/Data

From 2012.igem.org

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<h2>Reporters and promotors</h2>
<h2>Reporters and promotors</h2>
<p> <ul>
<p> <ul>
-
<li><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812030">K812030:</a>
+
<li><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812030">K812030:</a>Citrine reporter with a Kozak sequence for eukaryotic expression
 +
<li><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812031">K812031:</a>sfGFP with kozak sequence for eukaryotic expression
 +
<li><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812032">K812032:</a>mCFP with kozak sequence for eukaryotic expression
 +
<li><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812200">K812200:</a>Biobricked pSC2+ plasmid with Elastase promoter for frogs and chicken
 +
<li><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812300">K812300:</a>Biobricked pSC2+ plasmid with HSP70 promoter for frogs and chicken
 +
<li><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812233">K812233:</a>sfGFP in pCS2+ downstream to  elastase promoter
 +
<li><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812331">K812331:</a>sfGFP in pCS2+ downstream to  HSP70 promoter
 +
</ul>
 +
</p>
<h3>Characterization<h3> the information on characterized part can be found herein: <ul>
<h3>Characterization<h3> the information on characterized part can be found herein: <ul>
<li><a href="https://2012.igem.org/Team:Evry/FrenchFrog">FrenchFrog:</a>
<li><a href="https://2012.igem.org/Team:Evry/FrenchFrog">FrenchFrog:</a>
 +
<h1>Goldenbricks</h1>
<h1>Goldenbricks</h1>

Revision as of 00:11, 25 September 2012

Data page: Summary of what we have done this year (so far!)



New frog plasmids

Fig 1: Eucaryotic expression plasmid having both transcriptional and translational reagulational elements.

  • K812000: PCS2+ plasmid backbone for eukaryotic expression system containing regulational elements crucial for the efficacy of such system

The auxin system

The auxin receiver system

Fig 2: schemetic representation of the Auxin-receiver system

  • K812010:GFP fusion with the ubuquitinase E3 OsTirI recoginition domain which a main part of the degron system
  • K812012:OsTirI Ubiquitinase E3 for AID tagged protein degradation in the presence of auxin
  • K812013:

    The Auxin reciever system golden gate-assembled containing GFP-AID OsTirI polysistronic system for auxin detection in tadpole

The auxin emission system

Fig 3: Schematic representation of the Auxin two-step production cassette.

  • K812020:IAAH enzyme that catalyzes the second and last step towards auxin production
  • K812021:IAAM enzyme that catalyses the transformation of Tryptophan to Inodle-3-acetamide
  • K812014:Auxin production cassette containg both enzymes above which is monocystronic

Reporters and promotors

  • K812030:Citrine reporter with a Kozak sequence for eukaryotic expression
  • K812031:sfGFP with kozak sequence for eukaryotic expression
  • K812032:mCFP with kozak sequence for eukaryotic expression
  • K812200:Biobricked pSC2+ plasmid with Elastase promoter for frogs and chicken
  • K812300:Biobricked pSC2+ plasmid with HSP70 promoter for frogs and chicken
  • K812233:sfGFP in pCS2+ downstream to elastase promoter
  • K812331:sfGFP in pCS2+ downstream to HSP70 promoter

Characterization

the information on characterized part can be found herein:
  • FrenchFrog:

    Goldenbricks

    Summary

    Fig 4: Summary of the GoldenBrick procedure

    The GoldenBrick is a new assembly method for the partsregistry. If you want to know more, see this page.

    Our favourites parts

    • K812050: A GoldenBricked version of pSB1C3 with J04450 as negative cloning control
    • K812051: A GoldenBricked version of pSB1K3 with J04450 as negative cloning control

    The other parts we have created

    • K812050: A GoldenBricked version of pSB1C3 with J04450 as negative cloning control
    • K812051: A GoldenBricked version of pSB1K3 with J04450 as negative cloning control
    • K812053: A GoldenBricked version of the strong RBS B0034
    • K812054: A GoldenBricked version of the RFP E1010
    • K812055: A GoldenBricked version of the terminator B0015
    • K812056: A GoldenBricked version of the pLac R0010 promoter
    • K812057: A GoldenBricked of an sfGFP protein
    • K812058: A GoldenBricked of medium strenght RBS J61107
    • K812059: A GoldenBricked of week RBS J61117

    say we are testing them

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