Team:Evry/Data

From 2012.igem.org

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<h1>New frog plasmids</h1>
<h1>New frog plasmids</h1>
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<div class="center"><div class="thumb tnone"><div class="thumbinner" style="width:302px;"><a href="/File:PSC2_plasmid.png" class="image"><img alt="" src="http://2012.igem.org/wiki/images/9/90/PSC2_plasmid.png" width="300" class="thumbimage" /></a>  <div class="thumbcaption"><div class="magnify"><a href="/File:PSC2_plasmid.png" class="internal" title="Enlarge"><img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="" /></a></div>Fig 3: a. Eucaryotic expression plasmid having both transcriptional and translational reagulational elements.</div></div></div></div>
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<div class="center"><div class="thumb tnone"><div class="thumbinner" style="width:302px;"><a href="/File:PSC2_plasmid.png" class="image"><img alt="" src="http://2012.igem.org/wiki/images/9/90/PSC2_plasmid.png" width="300" class="thumbimage" /></a>  <div class="thumbcaption"><div class="magnify"><a href="/File:PSC2_plasmid.png" class="internal" title="Enlarge"><img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="" /></a></div>Fig 1: Eucaryotic expression plasmid having both transcriptional and translational reagulational elements.</div></div></div></div>
<p>
<p>
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<h2>The auxin receiver system</h2>
<h2>The auxin receiver system</h2>
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<div class="center"><div class="thumb tnone"><div class="thumbinner" style="width:502px;"><a href="/File:Degron_part2.jpg" class="image"><img alt="" src="http://2012.igem.org/wiki/images/c/c4/Degron_part2.jpg" width="500" class="thumbimage" /></a>  <div class="thumbcaption"><div class="magnify"><a href="/File:Degron_part2.jpg" class="internal" title="Enlarge"><img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="" /></a></div>Fig 5: a. The vector, promoter, and terminator are in excess: the assembly produces mostly monosistrons. b. The protein sequences and RBS are in excess: the assembly produces mostly polysistrons.</div></div></div></div>
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<div class="center"><div class="thumb tnone"><div class="thumbinner" style="width:502px;"><a href="/File:Degron_part2.jpg" class="image"><img alt="" src="http://2012.igem.org/wiki/images/c/c4/Degron_part2.jpg" width="500" class="thumbimage" /></a>  <div class="thumbcaption"><div class="magnify"><a href="/File:Degron_part2.jpg" class="internal" title="Enlarge"><img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="" /></a></div>Fig 2: schemetic representation of the Auxin-receiver system</div></div></div></div>
<p>
<p>
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<h2>The auxin emission system</h2>
<h2>The auxin emission system</h2>
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<div class="center"><div class="thumb tnone"><div class="thumbinner" style="width:502px;"><a href="/DEGRON_PART_1_.jpg‎" class="image"><img alt="" src="http://2012.igem.org/wiki/images/4/4b/DEGRON_PART_1_.jpg" width="500" class="thumbimage" /></a>  <div class="thumbcaption"><div class="magnify"><a href="/DEGRON_PART_1_.jpg" class="internal" title="Enlarge"><img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="" /></a></div>Fig 5: a. The vector, promoter, and terminator are in excess: the assembly produces mostly monosistrons. b. The protein sequences and RBS are in excess: the assembly produces mostly polysistrons.</div></div></div></div>
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<div class="center"><div class="thumb tnone"><div class="thumbinner" style="width:502px;"><a href="/DEGRON_PART_1_.jpg‎" class="image"><img alt="" src="http://2012.igem.org/wiki/images/4/4b/DEGRON_PART_1_.jpg" width="500" class="thumbimage" /></a>  <div class="thumbcaption"><div class="magnify"><a href="/DEGRON_PART_1_.jpg" class="internal" title="Enlarge"><img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="" /></a></div>Fig 3: Schematic representation of the Auxin two-step production cassette.</div></div></div></div>
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<p> <ul>
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<li><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812020">K812020:</a>IAAH enzyme that catalyzes the second and last step towards auxin production
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<li><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812021">K812021:</a>IAAM enzyme that catalyses the transformation of Tryptophan to Inodle-3-acetamide
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<li><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812014">K812014:</a>Auxin production cassette containg both enzymes above which is monocystronic
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</ul>
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</p>
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List of the parts with links to the registry
 
Which ones are characterized and result in one sentence
Which ones are characterized and result in one sentence

Revision as of 23:49, 24 September 2012

Data page: Summary of what we have done this year (so far!)



New frog plasmids

Fig 1: Eucaryotic expression plasmid having both transcriptional and translational reagulational elements.

  • K812000: PCS2+ plasmid backbone for eukaryotic expression system containing regulational elements crucial for the efficacy of such system

One characterization image

One plasmid map

The auxin system

The auxin receiver system

Fig 2: schemetic representation of the Auxin-receiver system

  • K812010:GFP fusion with the ubuquitinase E3 OsTirI recoginition domain which a main part of the degron system
  • K812012:OsTirI Ubiquitinase E3 for AID tagged protein degradation in the presence of auxin
  • K812013:

    The Auxin reciever system golden gate-assembled containing GFP-AID OsTirI polysistronic system for auxin detection in tadpole

The auxin emission system

Fig 3: Schematic representation of the Auxin two-step production cassette.

  • K812020:IAAH enzyme that catalyzes the second and last step towards auxin production
  • K812021:IAAM enzyme that catalyses the transformation of Tryptophan to Inodle-3-acetamide
  • K812014:Auxin production cassette containg both enzymes above which is monocystronic

Which ones are characterized and result in one sentence

Goldenbricks

Summary

Fig 4: Summary of the GoldenBrick procedure

The GoldenBrick is a new assembly method for the partsregistry. If you want to know more, see this page.

Our favourites parts

  • K812050: A GoldenBricked version of pSB1C3 with J04450 as negative cloning control
  • K812051: A GoldenBricked version of pSB1K3 with J04450 as negative cloning control

The other parts we have created

  • K812050: A GoldenBricked version of pSB1C3 with J04450 as negative cloning control
  • K812051: A GoldenBricked version of pSB1K3 with J04450 as negative cloning control
  • K812053: A GoldenBricked version of the strong RBS B0034
  • K812054: A GoldenBricked version of the RFP E1010
  • K812055: A GoldenBricked version of the terminator B0015
  • K812056: A GoldenBricked version of the pLac R0010 promoter
  • K812057: A GoldenBricked of an sfGFP protein
  • K812058: A GoldenBricked of medium strenght RBS J61107
  • K812059: A GoldenBricked of week RBS J61117

say we are testing them