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Team:Evry/Data
From 2012.igem.org
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| + | | <u>'''Auxin Degron System in Iadpoles'''</u><br> | ||
| + | Our System is based on a ligand-receptor communication where auxin is produced in a two step manner. following production, when auxin interacts with the auxin-inducible degradation(AID), the bound protein to the '''AID''' is thereafter degraded. In our system the AID-bound protein is the famous GFP reporter which upon loss of fluorescence, indicates the system's fidelity ).<br><br> | ||
| - | + | ''The following Diagram is a schematic representation of the '''Auxin-sensing System''' '''along with the main necessary interacting parts in the system submitted by our team: | |
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| - | + | | '''Data For Our Favorite New Parts''' | |
| - | + | # [http://partsregistry.org/Part:BBa_K812000 Main Page] - '''Eukaryotic exoression vector for tadpole injections , BBa_K812000''': This vector has all the prerequisites for eukaryotic trasncriptiona like 5' and 3' Untranslated regions implicated in effective transcriptional regulations . On top of this, it has all the requirements for amplification of any casette in bacteria. | |
| + | # [http://partsregistry.org/Part:BBa_BBa_K812000 Main Page] - '''Auxin production cassette, BBa_K812000''': The Ligand Production Cassette | ||
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| - | + | '''''Auxin Production System''''[[Image:DEGRON_PART_1_.jpg]] | |
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| + | # [http://partsregistry.org/Part:BBa_K812013 Main Page] - '''BAR Bad Odor Receptor, BBa_K812013''': The Auxin -induced degradation system of GFP which includes the GFP fused with AID upstream to OSTir1 which upon expression they form the auxin degradation system. Consequently, after interaction of auxin GFP will be degraded | ||
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| - | + | '''Auxin-Induced Degradation System Response Cassette'''<br>[[Image:Degron part2.jpg]] | |
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| - | + | | '''Golden Brick Parts''' | |
| - | + | # [http://partsregistry.org/Part:BBa_J45119:Experience Experience] - '''Wintergreen odor enzyme generator, BBa_J45119''' (MIT, iGEM 2006): 98 out of 100 volunteer subjects standing up to 5 feet away from the bacterial cultures could distinguish wintergreen-producing bacteria from negative controls. | |
| + | # [http://partsregistry.org/Part:BBa_J61110:Experience Experience] - '''RBS, BBa_J61110''' (Arkin Lab, 2007): Of the 5 RBS Parts we tested, this RBS works best for expressing yellow fluorescent protein-tagged BAR | ||
| + | | [[Image:KAH_053111_textbubble3.jpg|Text bubble 3]] | ||
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| + | | '''We've Also Characterized the Following Parts''' | ||
| + | # [http://partsregistry.org/Part:BBa_XXXXX Main Page] - '''Air Freshilizor, BBa_XXXXX''': Our mathematical model predicts that the threshold of activation is 10 parts per billion, the concentration of Butanethiol that humans can typically smell | ||
| + | | [[Image:KAH_053111_textbubble4.jpg|Text bubble 4]] | ||
| + | |} | ||
| - | + | {| style="background: #A8E4A0;" | |
| - | + | |- | |
| - | + | |In this example, this fictional team ran out of time before they could test the complete device and use it as a synthetic air freshener. However, they made progress testing their new promoter by using GFP and they did some mathematical modeling to understand the behavior of the system. They also conducted a test to see how far the wintergreen scent could travel from a flask of culture.<br><br> | |
| - | + | <u>Detailed Guidelines</u><br> | |
| - | + | * Data Figures: The data itself (graphs, charts, photos, etc.) must appear on the Registry page for the relevant Part, not in the Data Page itself. Please make figure images a reasonable size. Include a clear and descriptive figure legend. See [http://partsregistry.org/Part:BBa_J45119:Experience http://partsregistry.org/Part:BBa_J45119:Experience] for a good example. | |
| - | < | + | * Credit: The data linked to your Data Page must be data produced by your team. In the figure legends for your data in the Registry, include your team's name and the year the data was produced. |
| - | < | + | * Navigation: Add a clearly visible link on your team Wiki main page that points to your Data Page. |
| - | </ | + | * Data for brand new Parts: figures (charts, graphs, cell images, simulation graphs, model equations, etc.) and figure legends for New Parts must be posted on the '''Main Page''' section of the Part's Registry entry. |
| - | </ | + | * New data for pre-existing Parts made by other teams: must be posted on the '''Experience''' page under the '''Characterization''' section of the Part's Registry entry. Be sure to include your team name and year in the figure legend (example: '''Fictional Team 2011'''. Legend title. Legend text) |
| + | * New data for pre-existing Parts made by your school/ team in a previous year: If the pre-existing part was made by your school/ team during a previous year, you can add the data to the Main Page and indicate in the figure legend that your data is new (i.e., "Updated, 2011" or "New data, 2011") | ||
| + | * "Old" data from the literature: If you have collected descriptive measurements from the literature in order to help users better understand the function of your part, you may include that data on your Part page and you must credit the source.<br><br> | ||
| + | |} | ||
| + | <script type="text/javascript">writeFooter()</script> | ||
</html> | </html> | ||
Revision as of 16:12, 24 September 2012
Our System is based on a ligand-receptor communication where auxin is produced in a two step manner. following production, when auxin interacts with the auxin-inducible degradation(AID), the bound protein to the '''AID''' is thereafter degraded. In our system the AID-bound protein is the famous GFP reporter which upon loss of fluorescence, indicates the system's fidelity ).
''The following Diagram is a schematic representation of the '''Auxin-sensing System''' '''along with the main necessary interacting parts in the system submitted by our team: |} {| |- | |- | '''Data For Our Favorite New Parts''' # [http://partsregistry.org/Part:BBa_K812000 Main Page] - '''Eukaryotic exoression vector for tadpole injections , BBa_K812000''': This vector has all the prerequisites for eukaryotic trasncriptiona like 5' and 3' Untranslated regions implicated in effective transcriptional regulations . On top of this, it has all the requirements for amplification of any casette in bacteria. # [http://partsregistry.org/Part:BBa_BBa_K812000 Main Page] - '''Auxin production cassette, BBa_K812000''': The Ligand Production Cassette '''''Auxin Production System''''[[Image:DEGRON_PART_1_.jpg]] # [http://partsregistry.org/Part:BBa_K812013 Main Page] - '''BAR Bad Odor Receptor, BBa_K812013''': The Auxin -induced degradation system of GFP which includes the GFP fused with AID upstream to OSTir1 which upon expression they form the auxin degradation system. Consequently, after interaction of auxin GFP will be degraded '''Auxin-Induced Degradation System Response Cassette'''
[[Image:Degron part2.jpg]] |- | '''Golden Brick Parts''' # [http://partsregistry.org/Part:BBa_J45119:Experience Experience] - '''Wintergreen odor enzyme generator, BBa_J45119''' (MIT, iGEM 2006): 98 out of 100 volunteer subjects standing up to 5 feet away from the bacterial cultures could distinguish wintergreen-producing bacteria from negative controls. # [http://partsregistry.org/Part:BBa_J61110:Experience Experience] - '''RBS, BBa_J61110''' (Arkin Lab, 2007): Of the 5 RBS Parts we tested, this RBS works best for expressing yellow fluorescent protein-tagged BAR | [[Image:KAH_053111_textbubble3.jpg|Text bubble 3]] |- | '''We've Also Characterized the Following Parts''' # [http://partsregistry.org/Part:BBa_XXXXX Main Page] - '''Air Freshilizor, BBa_XXXXX''': Our mathematical model predicts that the threshold of activation is 10 parts per billion, the concentration of Butanethiol that humans can typically smell | [[Image:KAH_053111_textbubble4.jpg|Text bubble 4]] |} {| style="background: #A8E4A0;" |- |In this example, this fictional team ran out of time before they could test the complete device and use it as a synthetic air freshener. However, they made progress testing their new promoter by using GFP and they did some mathematical modeling to understand the behavior of the system. They also conducted a test to see how far the wintergreen scent could travel from a flask of culture.
Detailed Guidelines
* Data Figures: The data itself (graphs, charts, photos, etc.) must appear on the Registry page for the relevant Part, not in the Data Page itself. Please make figure images a reasonable size. Include a clear and descriptive figure legend. See [http://partsregistry.org/Part:BBa_J45119:Experience http://partsregistry.org/Part:BBa_J45119:Experience] for a good example. * Credit: The data linked to your Data Page must be data produced by your team. In the figure legends for your data in the Registry, include your team's name and the year the data was produced. * Navigation: Add a clearly visible link on your team Wiki main page that points to your Data Page. * Data for brand new Parts: figures (charts, graphs, cell images, simulation graphs, model equations, etc.) and figure legends for New Parts must be posted on the '''Main Page''' section of the Part's Registry entry. * New data for pre-existing Parts made by other teams: must be posted on the '''Experience''' page under the '''Characterization''' section of the Part's Registry entry. Be sure to include your team name and year in the figure legend (example: '''Fictional Team 2011'''. Legend title. Legend text) * New data for pre-existing Parts made by your school/ team in a previous year: If the pre-existing part was made by your school/ team during a previous year, you can add the data to the Main Page and indicate in the figure legend that your data is new (i.e., "Updated, 2011" or "New data, 2011") * "Old" data from the literature: If you have collected descriptive measurements from the literature in order to help users better understand the function of your part, you may include that data on your Part page and you must credit the source.
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