Team:Evry/BXcom
From 2012.igem.org
(Difference between revisions)
Line 9: | Line 9: | ||
<h2>Steps</h2> | <h2>Steps</h2> | ||
- | <center><img src="https://static.igem.org/mediawiki/2012/e/eb/Fig1.png"/></center> | + | |
- | <center><img src="https://static.igem.org/mediawiki/2012/d/da/Fig2_evry.png"/></center> | + | Our idea was to use previous biobricks from Imperial College 2011 (BBa_K515100). Indeed, they managed to express in Escherichia coli the genes enconding the IAA-producing pathway from Pseudomonas savastanoi. Besides, we constructed a plasmid with a reporter (mRFP) as a witness to see how far the auxin production can go. |
+ | |||
+ | <center><img src="https://static.igem.org/mediawiki/2012/e/eb/Fig1.png"/></center><br> | ||
+ | <center><u>Figure 1: Delivery by bacteria engineered</u></center> | ||
+ | |||
+ | Once all the construction prepared/analyzed in DH5a bacteria, we prepared a mix with bacteria and MMR medium and LB medium. So in a 16 plates, wich each contains 3 tadpoles we proceedeed as: <br> | ||
+ | <center><img src="https://static.igem.org/mediawiki/2012/d/da/Fig2_evry.png"/></center><br> | ||
+ | |||
+ | <center><u>Figure 2: Fluorescence quantification</u></center> | ||
+ | |||
+ | The results show that, except for the control, all tadpoles are fluorescent. The Fig. shows also that the fluorescent occurs mainly in the stomach. | ||
+ | |||
+ | <h2>Conclusion</h2> | ||
+ | |||
+ | |||
+ | |||
<script type="text/javascript">writeFooter()</script> | <script type="text/javascript">writeFooter()</script> | ||
</html> | </html> |
Revision as of 09:57, 17 September 2012