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Team:Evry-Genopole/Notebook/June/28
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'''pCS2+ : EcoRI/XbaI''' <br/> | '''pCS2+ : EcoRI/XbaI''' <br/> | ||
2,5microL buffer Orange + 0,25microL BSA + 18,5microL ddH2O<br/> | 2,5microL buffer Orange + 0,25microL BSA + 18,5microL ddH2O<br/> | ||
| - | '''=> | + | '''=> 3h, 37°C''' |
Revision as of 14:13, 29 June 2012
Promoters & Reporters workgroup
unit= ng/microliter
CFP : 240,1
GFP : 209,1
RFP : 142,8
YFP : 196,0
pCS2+ : 362,7
MiniPrep digestion
CFP, GFP, RFP, YFP : EcoRI/SpeI(BcuI)
Supermix: 10microL buffer Red + 1microL BSA + 74microL
Mix for each sample : 21,25microL supermix + 1microL DNA + 0,5microL EcoRI + 0,5microL BcuI
pCS2+ : EcoRI/XbaI
2,5microL buffer Orange + 0,25microL BSA + 18,5microL ddH2O
=> 3h, 37°C
