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Team:Evry-Genopole/Notebook/June/28
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<FONT SIZE=3>'''MiniPrep digestion'''</FONT> <br/> | <FONT SIZE=3>'''MiniPrep digestion'''</FONT> <br/> | ||
| - | '''CFP, GFP, RFP, YFP : | + | '''CFP, GFP, RFP, YFP : EcoRI/SpeI(BcuI)'''<br/> |
Supermix: 10microL buffer Red + 1microL BSA + 74microL<br/> | Supermix: 10microL buffer Red + 1microL BSA + 74microL<br/> | ||
Mix for each sample : 21,25microL supermix + 1microL DNA + 0,5microL EcoRI + 0,5microL BcuI<br/> | Mix for each sample : 21,25microL supermix + 1microL DNA + 0,5microL EcoRI + 0,5microL BcuI<br/> | ||
Revision as of 10:00, 29 June 2012
Promoters & Reporters workgroup
unit= ng/microliter
CFP : 240,1
GFP : 209,1
RFP : 142,8
YFP : 196,0
pCS2+ : 362,7
MiniPrep digestion
CFP, GFP, RFP, YFP : EcoRI/SpeI(BcuI)
Supermix: 10microL buffer Red + 1microL BSA + 74microL
Mix for each sample : 21,25microL supermix + 1microL DNA + 0,5microL EcoRI + 0,5microL BcuI
pCS2+ : EcoRI/XbaI
2,5microL buffer Orange + 0,25microL BSA + 18,5microL ddH2O
=> 1,5h, 37°C
