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| - | <font size="6">
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| - | Human Practice
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| - | <br>
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| - | <font size="5">
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| - | <font color=orange>
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| - | ・E.create!
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| - | <br>
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| - | <br>
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| - | <font color=red>
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| - | Our motivation
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| - | <font color=black>
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| - | <font size="3">
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| - | <p>
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| - | We made a BioBrick card game “E.create” to introduce to a number of people
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| - | the enjoyability of synthetic biology. Nobody in our team had learned synthetic biology until
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| - | we started our iGEM project. We thought from our experiences that the construction of the plasmids
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| - | is the most interesting process. We hope that everybody who is interested in life sciences and
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| - | genetic engineering can enjoy learning synthetic biology by playing with E.create!
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| - | </p>
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| - | <br>
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| - | <p>
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| - | E.create is a puzzle game. The kit contains cards with illustrations on them.
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| - | Each card corresponds to one of the gene parts including:
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| - | </p>
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| - | <font color=blue>
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| - | ・Promoter,
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| - | <br>
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| - | ・RBS (Ribosome Binding Site),
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| - | <br>
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| - | ・Protein coding regions,
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| - | <br>
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| - | ・Terminator,
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| - | <br>
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| - | ・Ori (Origin), and
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| - | <br>
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| - | ・Drug registance genes (KmR, CmR and AmpR).
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| - | <br>
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| - | <br>
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| - | <font color=skyblue>
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| - |
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| - | ☆ How to play
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| - | <font color=black>
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| - | <p>
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| - | Players will challenge to constract one or more plasmid(s) that should be required to
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| - | transform ''E.coli'' to give properties presented by the puzzle master. An example is shown below.
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| - | Let’s make it!
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| - | </p>
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| - | <br>
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| - | Ex. This ''E.coli'' can express GFP constitutively and has Km resistance.
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| - | <img src="https://2012.igem.org/File:%E3%81%A9%E3%82%84%EF%BC%92.png"
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| - | width="500px">
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| - | <br>
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| - | <p>
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| - | On the other day, a few members of our team visited a high school to do educational activity and play E.create together. We taught about 50 students who are learning biology. Thank to everyone!
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| - | </p>
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| - | <font size="5">
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| - | <font color=red>
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| - | Evaluation of our activities above.
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| - | <br>
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| - | <font size="3">
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| - | We asked the participants in our educational activities the following questions.
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| - | <br>
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| - | Questions about gene recombination__
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| - | <br>
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| - | 1.Before our lecture and playing E.create
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| - | Q1. Do you know gene recombination?
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| - | <br>
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| - | Q2. How do you feel toward gene recombination?
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| - | Q3. Do you think gene recombination is friendly?
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| - | <br>
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| - | 2.After our lecture and playing E.create
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| - | Q4. Did you change your idea to Q1?
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| - | <br>
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| - | Q5. Did you change your idea to Q2?
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| - | <br>
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| - | Q6. Did you change your idea to Q3?
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| - | Q7. Did you change your interest in gene recombination by comparison with before the lecture and playing E.create?
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| - | Answers to the questions
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| - | Answers to Q1 and Q4, Q2 and Q5, Q3 and Q6, and Q7 are shown below.
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| - | <font size="5"><font color=green><br>・Questionnaire
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| - | <groupparts>iGEM012 Ehime-Japan</groupparts>
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