Team:Ehime-Japan

From 2012.igem.org

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<font size="5">Overview</font>
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   Ehime-Japan team aims to create the communication systems which are circulatable. To realize our projects, we use cyanobacterial genes, Lux and Las systems, Lon protease and ssrA from mycoplasma. Green light sensor and red light sensor genes from Synechocystis are used as "Cell-cell signaling" like Lux and Las systems. To make a circular system, we develop a degradation system with Lon protease and ssrA from Mesoplasma florum.
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   E.co-mail + E.co-Domino + E.cold-heat sensor system
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= E.colingual !!
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→  Our projects
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    Ehime-Japan team tried three projects, and finally, we aims to  
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    create “E.colingual”, a tool through which we can know E.coli feelings.
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    It consists of three parts: Main, Connection, and Screen.
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    To realize our project, we used the light sensor genes from Synechocystis,  
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    Lon protease and ssrA tag from Mesoplasma florum. Also, a cell-cell signaling system
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    and the heat shock and cold shock promoters were used so as to construct
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    a foundational system of E.colingual.
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・E.co-mail (Connection)
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    “E.co-mail” is a communication tool with E.coli and an optical fiber.  
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    We would be able to transfer digital information depicted in E.coli
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    through a distance, and probably could send e-mails with this tool. 
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See more…
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・E.co-Domino (Screen)
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    We tried to reconstruct toppling dominoes with E.coli,
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    and we would be able to draw a movie of firework on LB plate!
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See more…
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・E.cold-heat sensing system (Main)
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    Main is comprised of two different kinds of E.coli transformants.
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    One is that feels heat and cold respond differently, producing different
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    signaling molecules. The other catches the signaling molecules and convert
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    the signal into the form of light. As a result, we can see a temperature change
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    around the E.coli. The light signals could be transferred through a distance
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    and be displayed if the Connection and Screen systems work.
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</p>  
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See more…
<BR>
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<font size="5">Background</font>
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・E.colingual
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<br>
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<br>
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<font size="3">
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<p>
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    It consists of three parts above. E.colingual, a tool through which
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    we can know E.coli feelings:“hot”,“cold”, “happy” ,or “dying” etc…
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    Also, by an optical fiber, the information of those feelings are transmitted
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    to someone in a distant place from a lab. Actually, we focused on E.coli
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    feelings, “hot” and “cold”.
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</p>
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<br>
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<br>
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<font size="5">
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→  New degradation system
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<br>
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<br>
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<font size="3">
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<p>
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    We used two different ssrA tags to make a circulatable communication system. One is from
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    M.florum recognized by the Lon protease from M.florum. The other is E.coli ssrA tag (LVA).
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    Because the sequences of those tags are not similar, the degradation systems function
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    independently.     
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</p>
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<br>
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See more…
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<br>
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<br>
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<br>
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<font size="5"> 
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→ New parts
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<p>
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    We made new BioBrick parts!
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  ・BBa_  : This part is cold shock promoter + lasI.
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</p>
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See more…
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  We thought that it sounds fun if a communication system is built with E. coli and it is used repeatedly. In addition, today, it is very important to recycle what someone used. So, we chose our projects.
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Revision as of 08:56, 25 September 2012

Ehime-Japan iGEM Team: Welcome









E.co-mail + E.co-Domino + E.cold-heat sensor system

= E.colingual !!

→ Our projects

Ehime-Japan team tried three projects, and finally, we aims to create “E.colingual”, a tool through which we can know E.coli feelings. It consists of three parts: Main, Connection, and Screen. To realize our project, we used the light sensor genes from Synechocystis, Lon protease and ssrA tag from Mesoplasma florum. Also, a cell-cell signaling system and the heat shock and cold shock promoters were used so as to construct a foundational system of E.colingual.



・E.co-mail (Connection)

“E.co-mail” is a communication tool with E.coli and an optical fiber. We would be able to transfer digital information depicted in E.coli through a distance, and probably could send e-mails with this tool.


See more…


・E.co-Domino (Screen)

We tried to reconstruct toppling dominoes with E.coli, and we would be able to draw a movie of firework on LB plate!


See more…


・E.cold-heat sensing system (Main)

Main is comprised of two different kinds of E.coli transformants. One is that feels heat and cold respond differently, producing different signaling molecules. The other catches the signaling molecules and convert the signal into the form of light. As a result, we can see a temperature change around the E.coli. The light signals could be transferred through a distance and be displayed if the Connection and Screen systems work.


See more…


・E.colingual

It consists of three parts above. E.colingual, a tool through which we can know E.coli feelings:“hot”,“cold”, “happy” ,or “dying” etc… Also, by an optical fiber, the information of those feelings are transmitted to someone in a distant place from a lab. Actually, we focused on E.coli feelings, “hot” and “cold”.



→ New degradation system

We used two different ssrA tags to make a circulatable communication system. One is from M.florum recognized by the Lon protease from M.florum. The other is E.coli ssrA tag (LVA). Because the sequences of those tags are not similar, the degradation systems function independently.


See more…


→ New parts

We made new BioBrick parts!
・BBa_ : This part is cold shock promoter + lasI.


See more…








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