http://2012.igem.org/wiki/index.php?title=Team:Cornell/testing/project/wetlab/4/5&feed=atom&action=historyTeam:Cornell/testing/project/wetlab/4/5 - Revision history2024-03-29T05:43:49ZRevision history for this page on the wikiMediaWiki 1.16.0http://2012.igem.org/wiki/index.php?title=Team:Cornell/testing/project/wetlab/4/5&diff=266816&oldid=prevD.Webster at 03:22, 4 October 20122012-10-04T03:22:53Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> Testing & Results</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> Testing & Results</div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <a href="https://2012.igem.org/Team:Cornell/testing/project/wetlab/4/7">Parts</a></ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <a href="https://2012.igem.org/Team:Cornell/testing/project/wetlab/4/1">Reactors</a></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <a href="https://2012.igem.org/Team:Cornell/testing/project/wetlab/4/1">Reactors</a></div></td></tr>
</table>D.Websterhttp://2012.igem.org/wiki/index.php?title=Team:Cornell/testing/project/wetlab/4/5&diff=260480&oldid=prevM.Simpson at 21:51, 3 October 20122012-10-03T21:51:07Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h3>Growth Assays</h3></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h3>Growth Assays</h3></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> Various engineered strains were tested with different amounts of sodium lactate in 20 hour or greater growth trials, in synthetic river media. After a few weeks of editing the protocol, a final procedure was developed for the <a href="https://static.igem.org/mediawiki/2012/6/6c/Growth_Assay_Protocol.pdf" target="_blank"><i>Shewanella</i> growth assay</a>. The results of the most successful experiment are shown below, with an OD600 minimum of 0.<del class="diffchange diffchange-inline">09 </del>reached for all three samples, with the sample appearing to still be on the rise. Therefore, a steady flow of 2% (w/v) sodium lactate could likely keep the cells alive and functioning for a long period of time in the reactor, though more testing will be needed to completely verify this claim. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> Various engineered strains were tested with different amounts of sodium lactate in 20 hour or greater growth trials, in synthetic river media. After a few weeks of editing the protocol, a final procedure was developed for the <a href="https://static.igem.org/mediawiki/2012/6/6c/Growth_Assay_Protocol.pdf" target="_blank"><i>Shewanella</i> growth assay</a>. The results of the most successful experiment are shown below, with an OD600 minimum of 0.<ins class="diffchange diffchange-inline">06 </ins>reached for all three samples, with the sample appearing to still be on the rise. Therefore, a steady flow of 2% (w/v) sodium lactate could likely keep the cells alive and functioning for a long period of time in the reactor, though more testing will be needed to completely verify this claim. </div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img class="inline" src="https://static.igem.org/mediawiki/2012/a/ac/Sal1GrowthCurve.jpg"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img class="inline" src="https://static.igem.org/mediawiki/2012/a/ac/Sal1GrowthCurve.jpg"></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><b> Fig. 1. Growth as OD600 over time is plotted for a 96 well-plate inoculated with engineered strain <i>S. oneidensis</i> Sal1 growing on 2% sodium lacate in Synthetic River Media. Three trials of the same strain are shown <del class="diffchange diffchange-inline">all </del>supporting an OD600 <del class="diffchange diffchange-inline">greater than </del>0.<del class="diffchange diffchange-inline">09</del>.</b><br><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><b> Fig. 1. Growth as OD600 over time is plotted for a 96 well-plate inoculated with engineered strain <i>S. oneidensis</i> Sal1 growing on 2% sodium lacate in Synthetic River Media. Three trials of the same strain are shown <ins class="diffchange diffchange-inline"> </ins>supporting an <ins class="diffchange diffchange-inline">average </ins>OD600 <ins class="diffchange diffchange-inline">of </ins>0.<ins class="diffchange diffchange-inline">098 (&sigma;=0.034)</ins>.</b><br><br></div></td></tr>
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</table>M.Simpsonhttp://2012.igem.org/wiki/index.php?title=Team:Cornell/testing/project/wetlab/4/5&diff=260445&oldid=prevM.Simpson at 21:46, 3 October 20122012-10-03T21:46:14Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><b> Fig. 1. Growth as OD600 over time is plotted for a 96 well-plate inoculated with engineered strain <i>S. oneidensis</i> Sal1 growing on <del class="diffchange diffchange-inline">0.02</del>% sodium lacate in Synthetic River Media. Three trials of the same strain are shown all supporting an OD600 greater than 0.09.</b><br><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><b> Fig. 1. Growth as OD600 over time is plotted for a 96 well-plate inoculated with engineered strain <i>S. oneidensis</i> Sal1 growing on <ins class="diffchange diffchange-inline">2</ins>% sodium lacate in Synthetic River Media. Three trials of the same strain are shown all supporting an OD600 greater than 0.09.</b><br><br></div></td></tr>
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</table>M.Simpsonhttp://2012.igem.org/wiki/index.php?title=Team:Cornell/testing/project/wetlab/4/5&diff=260434&oldid=prevM.Simpson at 21:44, 3 October 20122012-10-03T21:44:45Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h3>Growth Assays</h3></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h3>Growth Assays</h3></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> Various engineered strains were tested with </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> Various engineered strains were tested with <ins class="diffchange diffchange-inline">different amounts of sodium lactate in 20 hour or greater growth trials, in synthetic river media. After a few weeks of editing the protocol, a final procedure was developed for the <a href="https://static.igem.org/mediawiki/2012/6/6c/Growth_Assay_Protocol.pdf" target="_blank"><i>Shewanella</i> growth assay</a>. The results of the most successful experiment are shown below, with an OD600 minimum of 0.09 reached for all three samples, with the sample appearing to still be on the rise. Therefore, a steady flow of 2% (w/v) sodium lactate could likely keep the cells alive and functioning for a long period of time in the reactor, though more testing will be needed to completely verify this claim. </ins></div></td></tr>
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</table>M.Simpsonhttp://2012.igem.org/wiki/index.php?title=Team:Cornell/testing/project/wetlab/4/5&diff=260392&oldid=prevM.Simpson at 21:39, 3 October 20122012-10-03T21:39:22Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2012/8/88/Sal1Growth.jpg<del class="diffchange diffchange-inline">" width="twelve columns</del>"></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><b> Fig. 1. Growth as OD600 over time is plotted for a 96 well-plate inoculated with engineered strain <i>S. oneidensis</i> Sal1 growing on 0.02% sodium lacate in Synthetic River Media. Three trials of the same strain are shown all supporting an OD600 greater than 0.09.</b><br><br></ins></div></td></tr>
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</table>M.Simpsonhttp://2012.igem.org/wiki/index.php?title=Team:Cornell/testing/project/wetlab/4/5&diff=260358&oldid=prevM.Simpson at 21:35, 3 October 20122012-10-03T21:35:40Z<p></p>
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</table>M.Simpsonhttp://2012.igem.org/wiki/index.php?title=Team:Cornell/testing/project/wetlab/4/5&diff=260353&oldid=prevM.Simpson at 21:34, 3 October 20122012-10-03T21:34:40Z<p></p>
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</table>M.Simpsonhttp://2012.igem.org/wiki/index.php?title=Team:Cornell/testing/project/wetlab/4/5&diff=260349&oldid=prevM.Simpson at 21:34, 3 October 20122012-10-03T21:34:07Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h3>Growth Assays</h3></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h3>Growth Assays</h3></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <del class="diffchange diffchange-inline">Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <ins class="diffchange diffchange-inline">Various engineered strains were tested with </ins></div></td></tr>
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</table>M.Simpsonhttp://2012.igem.org/wiki/index.php?title=Team:Cornell/testing/project/wetlab/4/5&diff=260110&oldid=prevM.Simpson at 21:11, 3 October 20122012-10-03T21:11:35Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h3>Determining Food Tank Parameters</h3></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h3>Determining Food Tank Parameters</h3></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <del class="diffchange diffchange-inline">Lorem ipsum dolor sit amet</del>, <del class="diffchange diffchange-inline">consectetur adipisicing elit</del>, <del class="diffchange diffchange-inline">sed do eiusmod tempor incididunt ut labore et dolore magna aliqua</del>. <del class="diffchange diffchange-inline">Ut enim ad minim veniam</del>, <del class="diffchange diffchange-inline">quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat</del>. <del class="diffchange diffchange-inline">Duis aute irure dolor </del>in <del class="diffchange diffchange-inline">reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur</del>. <del class="diffchange diffchange-inline">Excepteur sint occaecat cupidatat non proident, sunt </del>in <del class="diffchange diffchange-inline">culpa qui officia deserunt mollit anim id est laborum</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <ins class="diffchange diffchange-inline">With the intention to make a biosensor capable of working self sufficiently for a long period of time</ins>, <ins class="diffchange diffchange-inline">maintaining the biological elements of the system is crucial. Therefore</ins>, <ins class="diffchange diffchange-inline">assays were performed to determine the parameters of the food tank</ins>. <ins class="diffchange diffchange-inline">Using only sodium lactate as a carbon source for <i>Shewanella</i></ins>, <ins class="diffchange diffchange-inline">the minimum concentration of nutrients that the bacteria needed to survive would be determined</ins>. <ins class="diffchange diffchange-inline">Considering the device was created with six liters of space for nutrients to be added, what would be the maximum concentration and miniumum flow rate that could be used by the device, to maintain successful results? The first step </ins>in <ins class="diffchange diffchange-inline">this is to simulate river conditions and add different "flow rates" of sodium lactate</ins>. <ins class="diffchange diffchange-inline">Then this determined concentration should be tested for biosensing ability </ins>in <ins class="diffchange diffchange-inline">a later reactor test</ins>. </div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h3>Developing Synthetic River Media</h3></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h3>Developing Synthetic River Media</h3></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <del class="diffchange diffchange-inline">Lorem ipsum dolor sit amet, consectetur adipisicing elit</del>, <del class="diffchange diffchange-inline">sed do eiusmod tempor incididunt ut labore et dolore magna aliqua</del>. <del class="diffchange diffchange-inline">Ut enim ad minim veniam</del>, <del class="diffchange diffchange-inline">quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat</del>. <del class="diffchange diffchange-inline">Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur</del>. <del class="diffchange diffchange-inline">Excepteur sint occaecat cupidatat non proident</del>, <del class="diffchange diffchange-inline">sunt in culpa qui officia deserunt mollit anim id est laborum</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <ins class="diffchange diffchange-inline">The first step is to determine the contents of a river of interest. The Athabasca river was selected</ins>, <ins class="diffchange diffchange-inline">as it is of particular importance in the Oil Sands region of Alberta</ins>. <ins class="diffchange diffchange-inline">The information on various concentrations of ions</ins>, <ins class="diffchange diffchange-inline">water hardness, pH, etc</ins>. <ins class="diffchange diffchange-inline">came from the <a href="http://environment</ins>.<ins class="diffchange diffchange-inline">alberta.ca/apps/osip" target="_blank">Oil Sands Information Portal</a>. Based on this information</ins>, <ins class="diffchange diffchange-inline">as well as available salts to use, a recipe for <a href="https://static.igem.org/mediawiki/2012/c/cb/Synthetic_River_Media.pdf" target="_blank">Synthetic River Media</a> was created to match the biologically significant parameters of the river's contents to the solution which would be used for later growth tests</ins>. </div></td></tr>
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</table>M.Simpsonhttp://2012.igem.org/wiki/index.php?title=Team:Cornell/testing/project/wetlab/4/5&diff=260004&oldid=prevM.Simpson at 21:00, 3 October 20122012-10-03T21:00:01Z<p></p>
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