http://2012.igem.org/wiki/index.php?title=Team:Cornell/testing/notebook/wetlab/1&feed=atom&action=historyTeam:Cornell/testing/notebook/wetlab/1 - Revision history2024-03-28T22:20:06ZRevision history for this page on the wikiMediaWiki 1.16.0http://2012.igem.org/wiki/index.php?title=Team:Cornell/testing/notebook/wetlab/1&diff=267904&oldid=prevT.Su at 03:54, 4 October 20122012-10-04T03:54:38Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> Having finished our bootcamp, we started working in our lab space in Weill Hall. This week, we began our work to construct our salicylate reporter plasmids using a sensing region including the transcriptional activator nahR. We also continued our work with the Gibson mutagenesis of the nah operon (which degrades naphthalene to salicylate) by attempting to confirm successful construction. We also began our adventure in attempting to transform into Shewanella via electroporation. <a href="#" class="technical-desc" for="#technical-desc1" style="display:block;margin-top:20px;">Daily Details</a></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> Having finished our bootcamp, we started working in our lab space in Weill Hall. This week, we began our work to construct our salicylate reporter plasmids using a sensing region including the transcriptional activator nahR. We also continued our work with the Gibson mutagenesis of the nah operon (which degrades naphthalene to salicylate) by attempting to confirm successful construction. We also began our adventure in attempting to transform into Shewanella via electroporation. <a href="#" class="technical-desc" for="#technical-desc1" style="display:block;margin-top:20px;">Daily Details</a></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <br><b>June 27th, Wednesday</b></br> <br>This morning, Steven made glycerol stocks of the 3 Gibson colonies grown overnight. He then did minipreps on the 3 Gibson colonies followed by double digests using PstI and KpnI to check whether the Gibson procedure was successful. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <br><b>June 27th, Wednesday</b></br> <br>This morning, Steven made glycerol stocks of the 3 Gibson colonies grown overnight. He then did minipreps on the 3 Gibson colonies followed by double digests using PstI and KpnI to check whether the Gibson procedure was successful. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Around noon, Steven and Dylan attempted the Myers and Myers Shewanella Electroporation with p17c (pSB3C5). Dylan and Swati then set up a Phusion PCR to amplify the salicylate-sensing region out of BBa_K228227 (to eventually control the expression of mtrB), before running a gel of the restriction digests of the Gibson products. However, our ladder ran strangely, and the gel was determined to be inconclusive in showing that our Gibson colonies appeared to be successful. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Around noon, Steven and Dylan attempted the Myers and Myers Shewanella Electroporation with p17c (pSB3C5). Dylan and Swati then set up a Phusion PCR to amplify the salicylate-sensing region out of BBa_K228227 (to eventually control the expression of mtrB), before running a gel of the restriction digests of the Gibson products. However, our ladder ran strangely, and the gel was determined to be inconclusive in showing that our Gibson colonies appeared to be successful. </div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> Having finished our bootcamp, we started working in our lab space in Weill Hall. This week, we began our work to construct our salicylate reporter plasmids using a sensing region including the transcriptional activator nahR. We also continued our work with the Gibson mutagenesis of the nah operon (which degrades naphthalene to salicylate) by attempting to confirm successful construction. We also began our adventure in attempting to transform into Shewanella via electroporation.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> Having finished our bootcamp, we started working in our lab space in Weill Hall. This week, we began our work to construct our salicylate reporter plasmids using a sensing region including the transcriptional activator nahR. We also continued our work with the Gibson mutagenesis of the nah operon (which degrades naphthalene to salicylate) by attempting to confirm successful construction. We also began our adventure in attempting to transform into Shewanella via electroporation.</div></td></tr>
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</table>T.Suhttp://2012.igem.org/wiki/index.php?title=Team:Cornell/testing/notebook/wetlab/1&diff=267851&oldid=prevT.Su: Undo revision 267729 by T.Su (talk)2012-10-04T03:53:38Z<p>Undo revision 267729 by <a href="/Special:Contributions/T.Su" title="Special:Contributions/T.Su">T.Su</a> (<a href="/wiki/index.php?title=User_talk:T.Su&action=edit&redlink=1" class="new" title="User talk:T.Su (page does not exist)">talk</a>)</p>
<a href="http://2012.igem.org/wiki/index.php?title=Team:Cornell/testing/notebook/wetlab/1&diff=267851&oldid=267729">Show changes</a>T.Suhttp://2012.igem.org/wiki/index.php?title=Team:Cornell/testing/notebook/wetlab/1&diff=267729&oldid=prevT.Su at 03:50, 4 October 20122012-10-04T03:50:41Z<p></p>
<a href="http://2012.igem.org/wiki/index.php?title=Team:Cornell/testing/notebook/wetlab/1&diff=267729&oldid=267642">Show changes</a>T.Suhttp://2012.igem.org/wiki/index.php?title=Team:Cornell/testing/notebook/wetlab/1&diff=267642&oldid=prevM.Simpson at 03:48, 4 October 20122012-10-04T03:48:05Z<p></p>
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</table>M.Simpsonhttp://2012.igem.org/wiki/index.php?title=Team:Cornell/testing/notebook/wetlab/1&diff=267614&oldid=prevM.Simpson at 03:46, 4 October 20122012-10-04T03:46:58Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <br><b>June 27th, Wednesday</b></br> <br>This morning, Steven made glycerol stocks of the 3 Gibson colonies grown overnight. He then did minipreps on the 3 Gibson colonies followed by double digests using PstI and KpnI to check whether the Gibson procedure was successful. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <br><b>June 27th, Wednesday</b></br> <br>This morning, Steven made glycerol stocks of the 3 Gibson colonies grown overnight. He then did minipreps on the 3 Gibson colonies followed by double digests using PstI and KpnI to check whether the Gibson procedure was successful. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Around noon, Steven and Dylan attempted the Myers and Myers Shewanella Electroporation with p17c (pSB3C5). Dylan and Swati then set up a Phusion PCR to amplify the salicylate-sensing region out of BBa_K228227 (to eventually control the expression of mtrB), before running a gel of the restriction digests of the Gibson products. However, our ladder ran strangely, and the gel was determined to be inconclusive in showing that our Gibson colonies appeared to be successful. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Around noon, Steven and Dylan attempted the Myers and Myers Shewanella Electroporation with p17c (pSB3C5). Dylan and Swati then set up a Phusion PCR to amplify the salicylate-sensing region out of BBa_K228227 (to eventually control the expression of mtrB), before running a gel of the restriction digests of the Gibson products. However, our ladder ran strangely, and the gel was determined to be inconclusive in showing that our Gibson colonies appeared to be successful. </div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <h3>Week 2</h3></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.</del></div></td><td colspan="2"> </td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <h3>Week 4</h3></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.</del></div></td><td colspan="2"> </td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <h3>Week <del class="diffchange diffchange-inline">1</del></h3></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <h3>Week <ins class="diffchange diffchange-inline">4</ins></h3></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> Having finished our bootcamp, we started working in our lab space in Weill Hall. This week, we began our work to construct our salicylate reporter plasmids using a sensing region including the transcriptional activator nahR. We also continued our work with the Gibson mutagenesis of the nah operon (which degrades naphthalene to salicylate) by attempting to confirm successful construction. We also began our adventure in attempting to transform into Shewanella via electroporation.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> Having finished our bootcamp, we started working in our lab space in Weill Hall. This week, we began our work to construct our salicylate reporter plasmids using a sensing region including the transcriptional activator nahR. We also continued our work with the Gibson mutagenesis of the nah operon (which degrades naphthalene to salicylate) by attempting to confirm successful construction. We also began our adventure in attempting to transform into Shewanella via electroporation.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Later that night, Steven and Spencer set up two transformations into PNNL electrocompetent Shewanella one at a voltage of 0.75 kV, and the other at 0.55 kV prescribed by Myers and Myers. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Later that night, Steven and Spencer set up two transformations into PNNL electrocompetent Shewanella one at a voltage of 0.75 kV, and the other at 0.55 kV prescribed by Myers and Myers. </div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <div class="nine columns"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <h3>Week 2</h3></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> It has been said that astronomy is a humbling and character-building experience. There is perhaps no better demonstration of the folly of human conceits than this distant image of our tiny world. To me, it underscores our responsibility to deal more kindly with one another, and to preserve and cherish the pale blue dot, the only home we've ever known.</del></div></td><td colspan="2"> </td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <h6>Daily Details:</h6></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.</del></div></td><td colspan="2"> </td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <div class="row"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <div class="nine columns"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <h3>Week 3</h3></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> It has been said that astronomy is a humbling and character-building experience. There is perhaps no better demonstration of the folly of human conceits than this distant image of our tiny world. To me, it underscores our responsibility to deal more kindly with one another, and to preserve and cherish the pale blue dot, the only home we've ever known.</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <div class="panel" style="background:white;margin-top: 20px;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <h6>Daily Details:</h6></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.</del></div></td><td colspan="2"> </td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <div class="row last-ele"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <div class="nine columns"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <h3>Week 4</h3></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> It has been said that astronomy is a humbling and character-building experience. There is perhaps no better demonstration of the folly of human conceits than this distant image of our tiny world. To me, it underscores our responsibility to deal more kindly with one another, and to preserve and cherish the pale blue dot, the only home we've ever known.</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <div class="panel" style="background:white;margin-top: 20px;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <h6>Daily Details:</h6></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.</del></div></td><td colspan="2"> </td></tr>
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</table>M.Simpsonhttp://2012.igem.org/wiki/index.php?title=Team:Cornell/testing/notebook/wetlab/1&diff=267562&oldid=prevM.Simpson at 03:45, 4 October 20122012-10-04T03:45:20Z<p></p>
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 03:45, 4 October 2012</td>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <h3>Week <del class="diffchange diffchange-inline">1</del></h3></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <h3>Week <ins class="diffchange diffchange-inline">4</ins></h3></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> Having finished our bootcamp, we started working in our lab space in Weill Hall. This week, we began our work to construct our salicylate reporter plasmids using a sensing region including the transcriptional activator nahR. We also continued our work with the Gibson mutagenesis of the nah operon (which degrades naphthalene to salicylate) by attempting to confirm successful construction. We also began our adventure in attempting to transform into Shewanella via electroporation. <a href="#" class="technical-desc" for="#technical-desc1" style="display:block;margin-top:20px;">Daily Details</a></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> Having finished our bootcamp, we started working in our lab space in Weill Hall. This week, we began our work to construct our salicylate reporter plasmids using a sensing region including the transcriptional activator nahR. We also continued our work with the Gibson mutagenesis of the nah operon (which degrades naphthalene to salicylate) by attempting to confirm successful construction. We also began our adventure in attempting to transform into Shewanella via electroporation. <a href="#" class="technical-desc" for="#technical-desc1" style="display:block;margin-top:20px;">Daily Details</a></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> <h3>Week 2</h3></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> It has been said that astronomy is a humbling and character-building experience. There is perhaps no better demonstration of the folly of human conceits than this distant image of our tiny world. To me, it underscores our responsibility to deal more kindly with one another, and to preserve and cherish the pale blue dot, the only home we've ever known.</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> <a href="#" class="technical-desc" for="#technical-desc7" style="display:block;margin-top:20px;">Daily Details</a></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> <div class="hide-me panel" style="background:white;margin-top: 20px;" id="technical-desc7"></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> <h6>Daily Details:</h6></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </div></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </div></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> <div class="three columns"></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> <img src="http://placehold.it/250x250/321852"></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </div></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </div></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> <div class="row"></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> <div class="nine columns"></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> <h3>Week 3</h3></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> It has been said that astronomy is a humbling and character-building experience. There is perhaps no better demonstration of the folly of human conceits than this distant image of our tiny world. To me, it underscores our responsibility to deal more kindly with one another, and to preserve and cherish the pale blue dot, the only home we've ever known.</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> <a href="#" class="technical-desc" for="#technical-desc2" style="display:block;margin-top:20px;">Daily Details</a></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> <div class="hide-me panel" style="background:white;margin-top: 20px;" id="technical-desc2"></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> <h6>Daily Details:</h6></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </div></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </div></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> <div class="three columns"></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> <img src="http://placehold.it/250x250/321852"></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </div></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </div></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> <div class="row last-ele"></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> <div class="nine columns"></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> <h3>Week 4</h3></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> It has been said that astronomy is a humbling and character-building experience. There is perhaps no better demonstration of the folly of human conceits than this distant image of our tiny world. To me, it underscores our responsibility to deal more kindly with one another, and to preserve and cherish the pale blue dot, the only home we've ever known.</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> <a href="#" class="technical-desc" for="#technical-desc3" style="display:block;margin-top:20px;">Daily Details</a></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> <div class="hide-me panel" style="background:white;margin-top: 20px;" id="technical-desc3"></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> <h6>Daily Details:</h6></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </div></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </div></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> <div class="three columns"></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> <img src="http://placehold.it/250x250/321852"></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </div></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </div></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div class="row last-ele"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div class="row last-ele"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div class="three columns"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div class="three columns"></div></td></tr>
</table>M.Simpsonhttp://2012.igem.org/wiki/index.php?title=Team:Cornell/testing/notebook/wetlab/1&diff=267481&oldid=prevCpaduano: Undo revision 267443 by Cpaduano (talk)2012-10-04T03:42:55Z<p>Undo revision 267443 by <a href="/Special:Contributions/Cpaduano" title="Special:Contributions/Cpaduano">Cpaduano</a> (<a href="/wiki/index.php?title=User_talk:Cpaduano&action=edit&redlink=1" class="new" title="User talk:Cpaduano (page does not exist)">talk</a>)</p>
<a href="http://2012.igem.org/wiki/index.php?title=Team:Cornell/testing/notebook/wetlab/1&diff=267481&oldid=267443">Show changes</a>Cpaduanohttp://2012.igem.org/wiki/index.php?title=Team:Cornell/testing/notebook/wetlab/1&diff=267443&oldid=prevCpaduano at 03:42, 4 October 20122012-10-04T03:42:05Z<p></p>
<a href="http://2012.igem.org/wiki/index.php?title=Team:Cornell/testing/notebook/wetlab/1&diff=267443&oldid=261116">Show changes</a>Cpaduanohttp://2012.igem.org/wiki/index.php?title=Team:Cornell/testing/notebook/wetlab/1&diff=261116&oldid=prevT.Su at 22:51, 3 October 20122012-10-03T22:51:19Z<p></p>
<table style="background-color: white; color:black;">
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<td colspan='2' style="background-color: white; color:black;">Revision as of 22:51, 3 October 2012</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h6>Daily Details:</h6></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h6>Daily Details:</h6></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <del class="diffchange diffchange-inline">Lorem ipsum dolor sit amet</del>, <del class="diffchange diffchange-inline">consectetur adipisicing elit</del>, <del class="diffchange diffchange-inline">sed do eiusmod tempor incididunt ut labore et dolore magna aliqua</del>. <del class="diffchange diffchange-inline">Ut enim ad minim veniam</del>, <del class="diffchange diffchange-inline">quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat</del>. <del class="diffchange diffchange-inline">Duis aute irure dolor </del>in <del class="diffchange diffchange-inline">reprehenderit </del>in <del class="diffchange diffchange-inline">voluptate velit esse cillum dolore eu fugiat nulla pariatur</del>. <del class="diffchange diffchange-inline">Excepteur sint occaecat cupidatat non proident</del>, <del class="diffchange diffchange-inline">sunt </del>in <del class="diffchange diffchange-inline">culpa qui officia deserunt mollit anim id est laborum</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <ins class="diffchange diffchange-inline"><br><b>June 27th</ins>, <ins class="diffchange diffchange-inline">Wednesday</b></br> <br>This morning</ins>, <ins class="diffchange diffchange-inline">Steven made glycerol stocks of the 3 Gibson colonies grown overnight</ins>. <ins class="diffchange diffchange-inline">He then did minipreps on the 3 Gibson colonies followed by double digests using PstI and KpnI to check whether the Gibson procedure was successful. </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Around noon</ins>, <ins class="diffchange diffchange-inline">Steven and Dylan attempted the Myers and Myers Shewanella Electroporation with p17c (pSB3C5)</ins>. <ins class="diffchange diffchange-inline">Dylan and Swati then set up a Phusion PCR to amplify the salicylate-sensing region out of BBa_K228227 (to eventually control the expression of mtrB), before running a gel of the restriction digests of the Gibson products. However, our ladder ran strangely, and the gel was determined to be inconclusive </ins>in <ins class="diffchange diffchange-inline">showing that our Gibson colonies appeared to be successful. </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">That night, Spencer and Steven set up overnight cultures of JG700, nahR, and the 3 Gibson colonies. </br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><br><b>June 28th, Thursday</b></br><br>This morning, Dylan and Caleb did minipreps of all the cultures from the day before. They then set up a gel of Phusion PCR of nahR from Wednesday. </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">That afternoon, Swati set up a transformation of p14k into DH5a cells. The cells were recovered for 1 hour at 37C </ins>in <ins class="diffchange diffchange-inline">LB before plating</ins>. <ins class="diffchange diffchange-inline">Dylan set up sequencing of the Gibson products with our newly designed primers while Shweta gel extracted the nahR from the gel set up by Dylan and Caleb. After Shweta’s gel extraction</ins>, <ins class="diffchange diffchange-inline">Swati set up a double digestion of the product with EcoRI and AscI. </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">That night, Steven ran a gel of the nahR digest while Spencer set up overnight cultures of p14k, p16k, pSB3C5, and pBBRBB. </br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><br><b>June 29th, Friday</b></br><br>In the morning, Dylan set up a Vent PCR to amplify p21a (salicylate sensing region) and his previous Phusion PCR band. That afternoon, he gel purified the PCR products and set up a double digestion with EcoRI-HF and AscI. Spencer miniprepped the overnight cultures from Thursday. </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Later that night, Dylan set up another Phusion PCR to amplify the nah operon from Gibson colony 1 and to append Biobrick cutsites into the product for future ligation into pSB3C5. Swati started the gel extraction of p21a. </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><br><b>June 30th, Saturday</b></br><br>Today, Dylan started out the day by running the PCR of the Gibson colony 1 on a gel, gel extracted the band, and set up another Phusion PCR with an extension time of 3 minutes to get more of the construct. </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">That afternoon, Swati finished her p21a gel extraction and dephosphorylated pBBRBB+mtrB for ligation with p21a. Swati desalted the ligation on Millipore membrane paper and electroporated her ligation into DH5a. After letting the cells recover </ins>in <ins class="diffchange diffchange-inline">LB for 1 hour, Swati plated her ligations. </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Later that night, Steven and Spencer set up two transformations into PNNL electrocompetent Shewanella one at a voltage of 0.75 kV, and the other at 0.55 kV prescribed by Myers and Myers</ins>. <ins class="diffchange diffchange-inline"> </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </br></ins></div></td></tr>
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</table>T.Suhttp://2012.igem.org/wiki/index.php?title=Team:Cornell/testing/notebook/wetlab/1&diff=261101&oldid=prevT.Su at 22:50, 3 October 20122012-10-03T22:50:00Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h3>Week 1</h3></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h3>Week 1</h3></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <del class="diffchange diffchange-inline">Having finished </del>our <del class="diffchange diffchange-inline">bootcamp</del>, <del class="diffchange diffchange-inline">we started working </del>in our <del class="diffchange diffchange-inline">lab space in Weill Hall</del>. This <del class="diffchange diffchange-inline">week</del>, <del class="diffchange diffchange-inline">we began </del>our <del class="diffchange diffchange-inline">work </del>to <del class="diffchange diffchange-inline">construct our </del>salicylate <del class="diffchange diffchange-inline">reporter plasmids using a </del>sensing region <del class="diffchange diffchange-inline">including the transcriptional activator nahR</del>. <del class="diffchange diffchange-inline">We also continued our work </del>with the <del class="diffchange diffchange-inline">Gibson mutagenesis of </del>the nah operon <del class="diffchange diffchange-inline">(which degrades naphthalene </del>to <del class="diffchange diffchange-inline">salicylate) </del>by <del class="diffchange diffchange-inline">attempting </del>to <del class="diffchange diffchange-inline">confirm successful construction</del>. <del class="diffchange diffchange-inline">We also began our adventure </del>in <del class="diffchange diffchange-inline">attempting to transform </del>into Shewanella <del class="diffchange diffchange-inline">via electroporation</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <ins class="diffchange diffchange-inline"><br><b>June 27th, Wednesday</b></br> <br>This morning, Steven made glycerol stocks of the 3 Gibson colonies grown overnight. He then did minipreps on the 3 Gibson colonies followed by double digests using PstI and KpnI to check whether the Gibson procedure was successful. </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Around noon, Steven and Dylan attempted the Myers and Myers Shewanella Electroporation with p17c (pSB3C5). Dylan and Swati then set up a Phusion PCR to amplify the salicylate-sensing region out of BBa_K228227 (to eventually control the expression of mtrB), before running a gel of the restriction digests of the Gibson products. However, </ins>our <ins class="diffchange diffchange-inline">ladder ran strangely</ins>, <ins class="diffchange diffchange-inline">and the gel was determined to be inconclusive </ins>in <ins class="diffchange diffchange-inline">showing that </ins>our <ins class="diffchange diffchange-inline">Gibson colonies appeared to be successful</ins>. </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">That night, Spencer and Steven set up overnight cultures of JG700, nahR, and the 3 Gibson colonies. </br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><br><b>June 28th, Thursday</b></br><br></ins>This <ins class="diffchange diffchange-inline">morning</ins>, <ins class="diffchange diffchange-inline">Dylan and Caleb did minipreps of all the cultures from the day before. They then set up a gel of Phusion PCR of nahR from Wednesday. </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">That afternoon, Swati set up a transformation of p14k into DH5a cells. The cells were recovered for 1 hour at 37C in LB before plating. Dylan set up sequencing of the Gibson products with </ins>our <ins class="diffchange diffchange-inline">newly designed primers while Shweta gel extracted the nahR from the gel set up by Dylan and Caleb. After Shweta’s gel extraction, Swati set up a double digestion of the product with EcoRI and AscI. </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">That night, Steven ran a gel of the nahR digest while Spencer set up overnight cultures of p14k, p16k, pSB3C5, and pBBRBB. </br></ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><br><b>June 29th, Friday</b></br><br>In the morning, Dylan set up a Vent PCR </ins>to <ins class="diffchange diffchange-inline">amplify p21a (</ins>salicylate sensing region<ins class="diffchange diffchange-inline">) and his previous Phusion PCR band</ins>. <ins class="diffchange diffchange-inline">That afternoon, he gel purified the PCR products and set up a double digestion </ins>with <ins class="diffchange diffchange-inline">EcoRI-HF and AscI. Spencer miniprepped </ins>the <ins class="diffchange diffchange-inline">overnight cultures from Thursday. </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Later that night, Dylan set up another Phusion PCR to amplify </ins>the nah operon <ins class="diffchange diffchange-inline">from Gibson colony 1 and </ins>to <ins class="diffchange diffchange-inline">append Biobrick cutsites into the product for future ligation into pSB3C5. Swati started the gel extraction of p21a. </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><br><b>June 30th, Saturday</b></br><br>Today, Dylan started out the day </ins>by <ins class="diffchange diffchange-inline">running the PCR of the Gibson colony 1 on a gel, gel extracted the band, and set up another Phusion PCR with an extension time of 3 minutes </ins>to <ins class="diffchange diffchange-inline">get more of the construct</ins>. </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">That afternoon, Swati finished her p21a gel extraction and dephosphorylated pBBRBB+mtrB for ligation with p21a. Swati desalted the ligation on Millipore membrane paper and electroporated her ligation into DH5a. After letting the cells recover </ins>in <ins class="diffchange diffchange-inline">LB for 1 hour, Swati plated her ligations. </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Later that night, Steven and Spencer set up two transformations </ins>into <ins class="diffchange diffchange-inline">PNNL electrocompetent </ins>Shewanella <ins class="diffchange diffchange-inline">one at a voltage of 0.75 kV, and the other at 0.55 kV prescribed by Myers and Myers</ins>. <ins class="diffchange diffchange-inline"> </ins></div></td></tr>
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</table>T.Su