Team:Cornell/testing/notebook/strainlist

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<a href="http://2012.igem.org/wiki/index.php?title=Team:Cornell/testing/notebook/strainlist">Strain List</a>
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<a href="http://2012.igem.org/Team:Cornell/testing/protocols">Protocols</a>
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<div class="ten columns team-bios-container">
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<h2 class="centered">Strain List</h2>
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<tr>
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<th>CUGEM ID</th><th>Strain Species</th><th>Strain name</th><th>Brief Description</th><th>Plasmid conferred resistances</th><th>Strain Genotype</th><th>BioBrick Insert ID</th><th>Backbone Name</th><th>Plasmid details
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<td>S0000</td><td>E. coli</td><td>DH5a</td><td></td><td>N/A</td><td></td><td>N/A</td><td>N/A</td><td>N/A
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</td>
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</tr>
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<td>S0001</td><td>S. oneidensis </td><td>JG700</td><td></td><td>N/A</td><td>_mtrB</td><td>N/A</td><td>N/A</td><td>N/A
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</td>
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</tr>
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<tr>
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<td>S0002</td><td>S. oneidensis </td><td>JG1194</td><td></td><td>N/A</td><td>_Mtr</td><td>N/A</td><td>N/A</td><td>N/A
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</td>
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<td>S0003</td><td>S. oneidensis </td><td>JG1519</td><td></td><td>N/A</td><td>_Mtr/_mtrB</td><td>N/A</td><td>N/A</td><td>N/A
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</td>
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<td>S0004</td><td>S. oneidensis </td><td>JG1220</td><td></td><td>kan</td><td>JG700 (S1)</td><td>N/A</td><td>?</td><td>pmtrB
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</td>
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<td>S0005</td><td>S. oneidensis </td><td>JG1219</td><td></td><td>kan</td><td>JG700 (S1)</td><td>N/A</td><td>?</td><td>pmtrE
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</td>
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</tr>
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<tr>
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<td>S0006</td><td>S. oneidensis </td><td>JG1527</td><td></td><td>kan</td><td>JG1519 (S3)</td><td>N/A</td><td>?</td><td>pmtrB/mtrC/mtrA
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</td>
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</tr>
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<tr>
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<td>S0007</td><td>S. oneidensis </td><td>JG1531</td><td></td><td>kan</td><td>JG1519 (S3)</td><td>N/A</td><td>?</td><td>pmtrE/mtrC/mtrA
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</td>
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</tr>
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<tr>
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<td>S0008</td><td>S. oneidensis </td><td>JG1537</td><td>Backbone (control)</td><td>kan</td><td>JG1519 (S3)</td><td>N/A</td><td>pBBR-BB</td><td>Empty backbone
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</td>
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</tr>
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<tr>
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<td>S0009</td><td>E. coli</td><td>DH5a</td><td>Failure</td><td>amp</td><td>DH5a</td><td>N/A</td><td>pBMT-1</td><td>Gibson 1 (Failed nah operon)
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</td>
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</tr>
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<tr>
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<td>S0010</td><td>E. coli</td><td>DH5a</td><td>Failure</td><td>amp</td><td>DH5a</td><td>N/A</td><td>pBMT-1</td><td>Gibson 2 (Failed nah operon)
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</td>
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</tr>
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<tr>
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<td>S0011</td><td>E. coli</td><td>DH5a</td><td>Failure</td><td>amp</td><td>DH5a</td><td>N/A</td><td>pBMT-1</td><td>Gibson 3 (Failed nah operon)
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</td>
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</tr>
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<tr>
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<td>S0012</td><td>E. coli</td><td>DH5a</td><td>Backbone</td><td>kan</td><td>DH5a</td><td>N/A</td><td></td><td>pBBRBB-eGFP
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</td>
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</tr>
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<tr>
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<td>S0013</td><td>E. coli</td><td>DH5a</td><td>ARS Reporter</td><td>kan</td><td>DH5a</td><td>?</td><td>pBBR-BB</td><td>SP1 (arsR with BamHI in EcoRI and PstI sites on pBBRBB)
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</td>
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</tr>
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<tr>
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<td>S0014</td><td>E. coli</td><td>DH5a</td><td>ARS Reporter</td><td>kan</td><td>DH5a</td><td>?</td><td>pBBR-BB</td><td>SP2 (arsR with BamHI in EcoRI and PstI sites on pBBRBB)
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</td>
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</tr>
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<tr>
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<td>S0015</td><td>E. coli</td><td>DH5a</td><td>ARS Reporter</td><td>kan</td><td>DH5a</td><td>?</td><td>pBBR-BB</td><td>Shweta's (either SP1 or SP2?)
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</td>
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</tr>
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<tr>
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<td>S0016</td><td>E. coli</td><td>DH5a</td><td>ARS Reporter</td><td>kan</td><td>DH5a</td><td>?</td><td>pBBR-BB</td><td>TS1 (arsR w/out BamHI in EcoRI and PstI sites on pBBRBB)
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</td>
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</tr>
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<tr>
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<td>S0017</td><td>E. coli</td><td>DH5a</td><td>ARS Reporter</td><td>kan</td><td>DH5a</td><td>?</td><td>pBBR-BB</td><td>TS2 (arsR w/out BamHI in EcoRI and PstI sites on pBBRBB)
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</td>
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</tr>
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<tr>
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<td>S0018</td><td>E. coli</td><td>DH5a</td><td>Backbone</td><td>cm</td><td>DH5a</td><td>BBa_J04450</td><td>pSB3C5</td><td>mRFP1 in MCS; p15a origin;
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</td>
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</tr>
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<tr>
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<td>S0019</td><td>E. coli</td><td>WM3064</td><td>Conjugation strain</td><td>N/A</td><td>DAP auxotroph</td><td>N/A</td><td></td><td>
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</td>
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</tr>
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<tr>
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<td>S0020a</td><td>S. oneidensis </td><td>CUGEM001</td><td>ARS Reporter</td><td>kan</td><td>JG700 (S1)</td><td>?</td><td></td><td>ARS Reporter w/ RBS swap (Shweta's)
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</td>
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</tr>
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<tr>
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<td>S0021</td><td>P. putida</td><td>G7</td><td>Wildtype</td><td>N/A</td><td>NAH7</td><td>N/A</td><td></td><td>
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</td>
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</tr>
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<tr>
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<td>S0022</td><td>E. coli</td><td>DH5a</td><td>Salicylate sensing</td><td>amp</td><td>DH5a</td><td>BBa_K228227</td><td>pSB4A5</td><td>"Salicylate sensing region (nahR and Pars); Plasmid map is not what you'd think, rely on our sequencing results."
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</td>
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</tr>
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<tr>
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<td>S0023</td><td>E. coli</td><td>DH5a</td><td>Nitrogen sensing</td><td>amp</td><td>DH5a</td><td>BBa_K216005</td><td>pSB1A2</td><td>"PyeaR promoter, responsive to nitrate, nitrite and nitric oxide"
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</td>
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</tr>
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<tr>
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<td>S0024</td><td>E. coli</td><td>DH5a</td><td>oriT</td><td>amp</td><td>DH5a</td><td>BBa_J01003</td><td>pSB1A2</td><td>"Origin of transfer, homologous to mob gene in PBRBB"
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</td>
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</tr>
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<tr>
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<td>S0025</td><td>E. coli</td><td>DH5a</td><td>Promoter</td><td>amp</td><td>DH5a</td><td>BBa_J23100</td><td>BBa_J61002</td><td>Constitutive promoter (1); mRFP downsteam; pMB1 origin;
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</td>
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</tr>
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<tr>
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<td>S0026</td><td>E. coli</td><td>DH5a</td><td>Promoter</td><td>amp</td><td>DH5a</td><td>BBa_J23101</td><td>BBa_J61002</td><td>Constitutive promoter (0.7); mRFP downsteam; pMB1 origin;
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</td>
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</tr>
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<tr>
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<td>S0027</td><td>E. coli</td><td>DH5a</td><td>Promoter</td><td>amp</td><td>DH5a</td><td>BBa_J23106</td><td>BBa_J61002</td><td>Constitutive promoter (0.4); mRFP downsteam; pMB1 origin;
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</td>
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</tr>
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<tr>
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<td>S0028</td><td>E. coli</td><td>DH5a</td><td>Promoter</td><td>amp</td><td>DH5a</td><td>BBa_J23105</td><td>BBa_J61002</td><td>Constitutive promoter (0.24); mRFP downsteam; pMB1 origin;
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</td>
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</tr>
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<tr>
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<td>S0029</td><td>E. coli</td><td>DH5a</td><td>Promoter</td><td>amp</td><td>DH5a</td><td>BBa_J23114</td><td>BBa_J61002</td><td>Constitutive promoter (0.1); mRFP downsteam; pMB1 origin;
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</td>
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</tr>
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<tr>
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<td>S0030</td><td>E. coli</td><td>DH5a</td><td>Promoter</td><td>amp</td><td>DH5a</td><td>BBa_J23103</td><td>BBa_J61002</td><td>Constitutive promoter (0.01); mRFP downsteam; pMB1 origin;
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</td>
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</tr>
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<tr>
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<td>S0031</td><td>E. coli</td><td>DH5a</td><td>Backbone</td><td>cm</td><td>DH5a</td><td>BBa_J04450</td><td>pSB1C3</td><td>mRFP1 in MCS; pMB1 origin;
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</td>
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</tr>
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<tr>
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<td>S0032</td><td>E. coli</td><td>DH5a</td><td>nah Operon</td><td>cm</td><td>DH5a</td><td></td><td>pSB1C3</td><td>nah operon in MCS; pBM1 origin;
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</td>
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</tr>
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<tr>
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<td>S0033</td><td>E. coli</td><td>DH5a</td><td>lac inducible mtrB</td><td>kan</td><td>DH5a</td><td>BBa_K098984</td><td>pSB3K3</td><td>lac indubible mtrB in MCS; p15a origin;
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</td>
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</tr>
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<tr>
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<td>S0034</td><td>E. coli</td><td>DH5a</td><td>SAL (Salicylate Reporter)</td><td>kan</td><td>DH5a</td><td></td><td>pBBR-BB</td><td>
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</td>
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</tr>
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<tr>
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<td>S0035</td><td>E. coli</td><td>DH5a</td><td>oriT + pSB3C5</td><td>cm</td><td>DH5a</td><td></td><td>pSB3C5</td><td>
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</td>
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</tr>
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<tr>
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<td>S0036</td><td>E. coli</td><td>DH5a</td><td>nah Operon + p29</td><td>amp</td><td>DH5a</td><td></td><td>BBa_J61002</td><td>
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</td>
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</tr>
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<tr>
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<td>S0037</td><td>E. coli</td><td>WM3064</td><td>p14 RFP</td><td>kan</td><td>WM3064</td><td></td><td>pBBRBB</td><td>
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</td>
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</tr>
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<tr>
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<td>S0038</td><td>E. coli</td><td>WM3064</td><td>p16 RFP</td><td>kan</td><td>WM3064</td><td></td><td>pBBRBB</td><td>
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</td>
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</tr>
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<tr>
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<td>S0039</td><td>E. coli</td><td>WM3064</td><td>p25BB</td><td>kan</td><td>WM3064</td><td></td><td>pBBRBB</td><td>
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</td>
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</tr>
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<tr>
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<td>S0040</td><td>E. coli</td><td>WM3064</td><td>p27BB</td><td>kan</td><td>WM3064</td><td></td><td>pBBRBB</td><td>
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</td>
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</tr>
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<tr>
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<td>S0041</td><td>E. coli</td><td>WM3064</td><td>p29BB</td><td>kan</td><td>WM3064</td><td></td><td>pBBRBB</td><td>
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</td>
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</tr>
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<tr>
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<td>S0042</td><td>E. coli</td><td>WM3064</td><td>WM_p14</td><td>kan</td><td>WM3064</td><td></td><td>pBBRBB</td><td>
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</td>
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</tr>
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<tr>
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<td>S0043</td><td>E. coli</td><td>WM3064</td><td>WM_p16</td><td>kan</td><td>WM3064</td><td></td><td>pBBRBB</td><td>
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</td>
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</tr>
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<tr>
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<td>S0044</td><td>E. coli</td><td>WM3064</td><td>WM_SAL1</td><td>kan</td><td>WM3064</td><td></td><td>pBBRBB</td><td>
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</td>
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</tr>
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<tr>
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<td>S0045</td><td>E. coli</td><td>WM3064</td><td>p29_nah_oriT_p17c</td><td>cm</td><td>WM3064</td><td></td><td>pSB3C5</td><td>Constitutively expressed nah operon w/ oriT
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</td>
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</tr>
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<tr>
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<td>S0046</td><td>E. coli</td><td>WM3064</td><td>SAL_RFP</td><td>kan</td><td>WM3064</td><td></td><td>pBBRBB</td><td>
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</td>
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</tr>
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<tr>
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<td>S0047</td><td>E. coli</td><td>WM3064</td><td>SAL2</td><td>kan</td><td>WM3064</td><td></td><td>pBBRBB</td><td>
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</td>
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</tr>
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<tr>
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<td>S0048</td><td>E. coli</td><td>WM3064</td><td>SAL2_RFP</td><td>kan</td><td>WM3064</td><td></td><td>pBBRBB</td>
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Revision as of 23:42, 3 October 2012

Strain List

CUGEM IDStrain SpeciesStrain nameBrief DescriptionPlasmid conferred resistancesStrain GenotypeBioBrick Insert IDBackbone NamePlasmid details
S0000E. coliDH5aN/AN/AN/AN/A
S0001S. oneidensis JG700N/A_mtrBN/AN/AN/A
S0002S. oneidensis JG1194N/A_MtrN/AN/AN/A
S0003S. oneidensis JG1519N/A_Mtr/_mtrBN/AN/AN/A
S0004S. oneidensis JG1220kanJG700 (S1)N/A?pmtrB
S0005S. oneidensis JG1219kanJG700 (S1)N/A?pmtrE
S0006S. oneidensis JG1527kanJG1519 (S3)N/A?pmtrB/mtrC/mtrA
S0007S. oneidensis JG1531kanJG1519 (S3)N/A?pmtrE/mtrC/mtrA
S0008S. oneidensis JG1537Backbone (control)kanJG1519 (S3)N/ApBBR-BBEmpty backbone
S0009E. coliDH5aFailureampDH5aN/ApBMT-1Gibson 1 (Failed nah operon)
S0010E. coliDH5aFailureampDH5aN/ApBMT-1Gibson 2 (Failed nah operon)
S0011E. coliDH5aFailureampDH5aN/ApBMT-1Gibson 3 (Failed nah operon)
S0012E. coliDH5aBackbonekanDH5aN/ApBBRBB-eGFP
S0013E. coliDH5aARS ReporterkanDH5a?pBBR-BBSP1 (arsR with BamHI in EcoRI and PstI sites on pBBRBB)
S0014E. coliDH5aARS ReporterkanDH5a?pBBR-BBSP2 (arsR with BamHI in EcoRI and PstI sites on pBBRBB)
S0015E. coliDH5aARS ReporterkanDH5a?pBBR-BBShweta's (either SP1 or SP2?)
S0016E. coliDH5aARS ReporterkanDH5a?pBBR-BBTS1 (arsR w/out BamHI in EcoRI and PstI sites on pBBRBB)
S0017E. coliDH5aARS ReporterkanDH5a?pBBR-BBTS2 (arsR w/out BamHI in EcoRI and PstI sites on pBBRBB)
S0018E. coliDH5aBackbonecmDH5aBBa_J04450pSB3C5mRFP1 in MCS; p15a origin;
S0019E. coliWM3064Conjugation strainN/ADAP auxotrophN/A
S0020aS. oneidensis CUGEM001ARS ReporterkanJG700 (S1)?ARS Reporter w/ RBS swap (Shweta's)
S0021P. putidaG7WildtypeN/ANAH7N/A
S0022E. coliDH5aSalicylate sensingampDH5aBBa_K228227pSB4A5"Salicylate sensing region (nahR and Pars); Plasmid map is not what you'd think, rely on our sequencing results."
S0023E. coliDH5aNitrogen sensingampDH5aBBa_K216005pSB1A2"PyeaR promoter, responsive to nitrate, nitrite and nitric oxide"
S0024E. coliDH5aoriTampDH5aBBa_J01003pSB1A2"Origin of transfer, homologous to mob gene in PBRBB"
S0025E. coliDH5aPromoterampDH5aBBa_J23100BBa_J61002Constitutive promoter (1); mRFP downsteam; pMB1 origin;
S0026E. coliDH5aPromoterampDH5aBBa_J23101BBa_J61002Constitutive promoter (0.7); mRFP downsteam; pMB1 origin;
S0027E. coliDH5aPromoterampDH5aBBa_J23106BBa_J61002Constitutive promoter (0.4); mRFP downsteam; pMB1 origin;
S0028E. coliDH5aPromoterampDH5aBBa_J23105BBa_J61002Constitutive promoter (0.24); mRFP downsteam; pMB1 origin;
S0029E. coliDH5aPromoterampDH5aBBa_J23114BBa_J61002Constitutive promoter (0.1); mRFP downsteam; pMB1 origin;
S0030E. coliDH5aPromoterampDH5aBBa_J23103BBa_J61002Constitutive promoter (0.01); mRFP downsteam; pMB1 origin;
S0031E. coliDH5aBackbonecmDH5aBBa_J04450pSB1C3mRFP1 in MCS; pMB1 origin;
S0032E. coliDH5anah OperoncmDH5apSB1C3nah operon in MCS; pBM1 origin;
S0033E. coliDH5alac inducible mtrBkanDH5aBBa_K098984pSB3K3lac indubible mtrB in MCS; p15a origin;
S0034E. coliDH5aSAL (Salicylate Reporter)kanDH5apBBR-BB
S0035E. coliDH5aoriT + pSB3C5cmDH5apSB3C5
S0036E. coliDH5anah Operon + p29ampDH5aBBa_J61002
S0037E. coliWM3064p14 RFPkanWM3064pBBRBB
S0038E. coliWM3064p16 RFPkanWM3064pBBRBB
S0039E. coliWM3064p25BBkanWM3064pBBRBB
S0040E. coliWM3064p27BBkanWM3064pBBRBB
S0041E. coliWM3064p29BBkanWM3064pBBRBB
S0042E. coliWM3064WM_p14kanWM3064pBBRBB
S0043E. coliWM3064WM_p16kanWM3064pBBRBB
S0044E. coliWM3064WM_SAL1kanWM3064pBBRBB
S0045E. coliWM3064p29_nah_oriT_p17ccmWM3064pSB3C5Constitutively expressed nah operon w/ oriT
S0046E. coliWM3064SAL_RFPkanWM3064pBBRBB
S0047E. coliWM3064SAL2kanWM3064pBBRBB
S0048E. coliWM3064SAL2_RFPkanWM3064pBBRBB