http://2012.igem.org/wiki/index.php?title=Team:Cornell/testing/notebook/drylab/2&feed=atom&action=historyTeam:Cornell/testing/notebook/drylab/2 - Revision history2024-03-29T14:28:59ZRevision history for this page on the wikiMediaWiki 1.16.0http://2012.igem.org/wiki/index.php?title=Team:Cornell/testing/notebook/drylab/2&diff=266904&oldid=prevS.Sureka at 03:25, 4 October 20122012-10-04T03:25:25Z<p></p>
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</table>S.Surekahttp://2012.igem.org/wiki/index.php?title=Team:Cornell/testing/notebook/drylab/2&diff=264905&oldid=prevR.Zhang at 02:14, 4 October 20122012-10-04T02:14:35Z<p></p>
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</table>R.Zhanghttp://2012.igem.org/wiki/index.php?title=Team:Cornell/testing/notebook/drylab/2&diff=257453&oldid=prevDanVLevine at 10:09, 3 October 20122012-10-03T10:09:52Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <a href="https://2012.igem.org/Team:Cornell/testing/notebook/drylab/1">Week 1</a></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <a href="https://2012.igem.org/Team:Cornell/testing/notebook/drylab/1">Week 1</a></div></td></tr>
</table>DanVLevinehttp://2012.igem.org/wiki/index.php?title=Team:Cornell/testing/notebook/drylab/2&diff=257060&oldid=prevDanVLevine at 09:13, 3 October 20122012-10-03T09:13:57Z<p></p>
<a href="http://2012.igem.org/wiki/index.php?title=Team:Cornell/testing/notebook/drylab/2&diff=257060&oldid=255971">Show changes</a>DanVLevinehttp://2012.igem.org/wiki/index.php?title=Team:Cornell/testing/notebook/drylab/2&diff=255971&oldid=prevDanVLevine at 07:24, 3 October 20122012-10-03T07:24:08Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <h3>Saturday</h3></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <h3>Saturday<ins class="diffchange diffchange-inline">, June 16, 2012</ins></h3></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <del class="diffchange diffchange-inline">BRIEFINGS</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <ins class="diffchange diffchange-inline">Focus: System Flow</ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><p></p>Decisions have been made! With flow method and battery selected, we move onto pumps.</ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h6>Entry:</h6></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h6>Entry:</h6></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <del class="diffchange diffchange-inline">ALLTHEDETAILS</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <ins class="diffchange diffchange-inline">Maneesh and Dylan made a presentation for discrete flow. The system seems to be complicated, as it involves pressure gradients and the use of feedback loops and modifiers to generate pressure equalization. Since simplicity is a top priority, we returned to continuous flow because it would be easier to implement and still fulfill the functional requirements. <p></p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">During further discussion, we did a back-of-the-envelope calculation for a continuous flow rate of water. It came out to be 0.03 milliliters per minute, which meant we needed a very precise, slow-acting pump. For the purpose of conserving energy, Dan found some low power pumps, including a $5 Chinese manufactured pump and a $20 500 GPH (gallons per hour) bilge pump. However, more research is required to find the optimal pump. We also thought about methods for introducing food into the stream. One idea was to use a worm-gear compressor to slowly push through our system.<p></p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Chie presented candidates for our biosensor battery. We selected a 100Ah deep cycle gel cell battery, after listening to suggestions from several professors. Lydia and others discussed meeting with ECE professors to figure out the logistics of the circuitry component. We have a rough plan for developing the potentiostat, which is vital for measuring changes in potential, or signal produced by the bacteria when they detect toxins in the water.<p></p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">#flow #pump #battery #potentiostat</ins></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <h3>Saturday</h3></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <h3>Saturday<ins class="diffchange diffchange-inline">, June 16, 2012</ins></h3></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <del class="diffchange diffchange-inline">ALLTHEDETAILS</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <ins class="diffchange diffchange-inline">Maneesh and Dylan made a presentation for discrete flow. The system seems to be complicated, as it involves pressure gradients and the use of feedback loops and modifiers to generate pressure equalization. Since simplicity is a top priority, we returned to continuous flow because it would be easier to implement and still fulfill the functional requirements. <p></p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">During further discussion, we did a back-of-the-envelope calculation for a continuous flow rate of water. It came out to be 0.03 milliliters per minute, which meant we needed a very precise, slow-acting pump. For the purpose of conserving energy, Dan found some low power pumps, including a $5 Chinese manufactured pump and a $20 500 GPH (gallons per hour) bilge pump. However, more research is required to find the optimal pump. We also thought about methods for introducing food into the stream. One idea was to use a worm-gear compressor to slowly push through our system.<p></p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Chie presented candidates for our biosensor battery. We selected a 100Ah deep cycle gel cell battery, after listening to suggestions from several professors. Lydia and others discussed meeting with ECE professors to figure out the logistics of the circuitry component. We have a rough plan for developing the potentiostat, which is vital for measuring changes in potential, or signal produced by the bacteria when they detect toxins in the water.<p></p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">#flow #pump #battery #potentiostat</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div class="three columns"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div class="three columns"></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div class="nine columns"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div class="nine columns"></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <h3>Saturday</h3></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <h3>Saturday<ins class="diffchange diffchange-inline">, June 16, 2012</ins></h3></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <del class="diffchange diffchange-inline">BRIEFINGS</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <ins class="diffchange diffchange-inline">Focus: System Flow</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><p></p>Decisions have been made! With flow method and battery selected, we move onto pumps.</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div class="panel" style="background:white;margin-top: 20px;"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div class="panel" style="background:white;margin-top: 20px;"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h6>Entry:</h6></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h6>Entry:</h6></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <del class="diffchange diffchange-inline">ALLTHEDETAILS</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <ins class="diffchange diffchange-inline">Maneesh and Dylan made a presentation for discrete flow. The system seems to be complicated, as it involves pressure gradients and the use of feedback loops and modifiers to generate pressure equalization. Since simplicity is a top priority, we returned to continuous flow because it would be easier to implement and still fulfill the functional requirements. <p></p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">During further discussion, we did a back-of-the-envelope calculation for a continuous flow rate of water. It came out to be 0.03 milliliters per minute, which meant we needed a very precise, slow-acting pump. For the purpose of conserving energy, Dan found some low power pumps, including a $5 Chinese manufactured pump and a $20 500 GPH (gallons per hour) bilge pump. However, more research is required to find the optimal pump. We also thought about methods for introducing food into the stream. One idea was to use a worm-gear compressor to slowly push through our system.<p></p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Chie presented candidates for our biosensor battery. We selected a 100Ah deep cycle gel cell battery, after listening to suggestions from several professors. Lydia and others discussed meeting with ECE professors to figure out the logistics of the circuitry component. We have a rough plan for developing the potentiostat, which is vital for measuring changes in potential, or signal produced by the bacteria when they detect toxins in the water.<p></p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">#flow #pump #battery #potentiostat</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></div></td></tr>
</table>DanVLevinehttp://2012.igem.org/wiki/index.php?title=Team:Cornell/testing/notebook/drylab/2&diff=255923&oldid=prevDanVLevine at 07:21, 3 October 20122012-10-03T07:21:00Z<p></p>
<a href="http://2012.igem.org/wiki/index.php?title=Team:Cornell/testing/notebook/drylab/2&diff=255923&oldid=255901">Show changes</a>DanVLevinehttp://2012.igem.org/wiki/index.php?title=Team:Cornell/testing/notebook/drylab/2&diff=255901&oldid=prevDanVLevine at 07:19, 3 October 20122012-10-03T07:19:27Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h6>Entry:</h6></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h6>Entry:</h6></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <del class="diffchange diffchange-inline">Lorem ipsum dolor sit amet</del>, <del class="diffchange diffchange-inline">consectetur adipisicing elit</del>, <del class="diffchange diffchange-inline">sed do eiusmod tempor incididunt ut labore et dolore magna aliqua</del>. <del class="diffchange diffchange-inline">Ut enim ad minim veniam</del>, <del class="diffchange diffchange-inline">quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat</del>. <del class="diffchange diffchange-inline">Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur</del>. <del class="diffchange diffchange-inline">Excepteur sint occaecat cupidatat non proident</del>, <del class="diffchange diffchange-inline">sunt in culpa qui officia deserunt mollit anim id est laborum</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <ins class="diffchange diffchange-inline">Today was a huge discussion day</ins>, <ins class="diffchange diffchange-inline">in which we continued our previous discussion on fluid flow through the system. Last time</ins>, <ins class="diffchange diffchange-inline">we leaned toward a discrete system because it would cut energy costs due to less activity</ins>. <ins class="diffchange diffchange-inline">However</ins>, <ins class="diffchange diffchange-inline">Dylan told us today that discrete flow would not work because the culture needs to maintain steady state</ins>. <ins class="diffchange diffchange-inline">According to the equations and graphs he drew on the whiteboard, discrete flow would not satisfy this requirement</ins>. <ins class="diffchange diffchange-inline">Any deviation from steady state would add noise to the electrical output from bacteria</ins>, <ins class="diffchange diffchange-inline">rendering the biosensor inaccurate. <p></p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Still, Maneesh and Dylan tried to design a discrete system that would work. A programmable fish food dispenser would periodically release food into the bioreactor that held the bacterial culture. And a microcontroller would be used to activate the water pump. However, the issue of maintaining steady state still lingered. <p></p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">On the whiteboard, we also came up with two other designs--a passive gravity fed system and a continuous model. For continuous flow, pumps would be constantly churning food and water into the bioreactor. The disadvantages are incredibly low flow rates (meaning higher-end machines) and greatest power consumption. <p></p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">In the passive system, food and water input from above the water level would flow down into the bioreactor. This design allowed for continuous flow and least energy consumption. However, the bulk of this system would need to be strictly underwater, so we would have to look into waterproofing</ins>. <ins class="diffchange diffchange-inline"><p></p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">#filtration #flow #gravity</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></div></td></tr>
</table>DanVLevinehttp://2012.igem.org/wiki/index.php?title=Team:Cornell/testing/notebook/drylab/2&diff=255876&oldid=prevDanVLevine at 07:17, 3 October 20122012-10-03T07:17:59Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h6>Entry:</h6></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h6>Entry:</h6></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <del class="diffchange diffchange-inline">Today was a huge discussion day</del>, <del class="diffchange diffchange-inline">in which we continued our previous discussion on fluid flow through the system. Last time</del>, <del class="diffchange diffchange-inline">we leaned toward a discrete system because it would cut energy costs due to less activity</del>. <del class="diffchange diffchange-inline">However</del>, <del class="diffchange diffchange-inline">Dylan told us today that discrete flow would not work because the culture needs to maintain steady state</del>. <del class="diffchange diffchange-inline">According to the equations and graphs he drew on the whiteboard, discrete flow would not satisfy this requirement</del>. <del class="diffchange diffchange-inline">Any deviation from steady state would add noise to the electrical output from bacteria</del>, <del class="diffchange diffchange-inline">rendering the biosensor inaccurate. <p></p></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <ins class="diffchange diffchange-inline">Lorem ipsum dolor sit amet</ins>, <ins class="diffchange diffchange-inline">consectetur adipisicing elit</ins>, <ins class="diffchange diffchange-inline">sed do eiusmod tempor incididunt ut labore et dolore magna aliqua</ins>. <ins class="diffchange diffchange-inline">Ut enim ad minim veniam</ins>, <ins class="diffchange diffchange-inline">quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat</ins>. <ins class="diffchange diffchange-inline">Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur</ins>. <ins class="diffchange diffchange-inline">Excepteur sint occaecat cupidatat non proident</ins>, <ins class="diffchange diffchange-inline">sunt in culpa qui officia deserunt mollit anim id est laborum</ins>.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Still, Maneesh and Dylan tried to design a discrete system that would work. A programmable fish food dispenser would periodically release food into the bioreactor that held the bacterial culture. And a microcontroller would be used to activate the water pump. However, the issue of maintaining steady state still lingered. <p></p></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">On the whiteboard, we also came up with two other designs--a passive gravity fed system and a continuous model. For continuous flow, pumps would be constantly churning food and water into the bioreactor. The disadvantages are incredibly low flow rates (meaning higher-end machines) and greatest power consumption. <p></p></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">In the passive system, food and water input from above the water level would flow down into the bioreactor. This design allowed for continuous flow and least energy consumption. However, the bulk of this system would need to be strictly underwater, so we would have to look into waterproofing</del>. <del class="diffchange diffchange-inline"><p></p></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">#filtration #flow #gravity</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></div></td></tr>
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</table>DanVLevinehttp://2012.igem.org/wiki/index.php?title=Team:Cornell/testing/notebook/drylab/2&diff=255851&oldid=prevDanVLevine at 07:16, 3 October 20122012-10-03T07:16:27Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h6>Entry:</h6></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h6>Entry:</h6></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <del class="diffchange diffchange-inline">Lorem ipsum dolor sit amet</del>, <del class="diffchange diffchange-inline">consectetur adipisicing elit</del>, <del class="diffchange diffchange-inline">sed do eiusmod tempor incididunt ut labore et dolore magna aliqua</del>. <del class="diffchange diffchange-inline">Ut enim ad minim veniam</del>, <del class="diffchange diffchange-inline">quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat</del>. <del class="diffchange diffchange-inline">Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur</del>. <del class="diffchange diffchange-inline">Excepteur sint occaecat cupidatat non proident</del>, <del class="diffchange diffchange-inline">sunt in culpa qui officia deserunt mollit anim id est laborum</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <ins class="diffchange diffchange-inline">Today was a huge discussion day</ins>, <ins class="diffchange diffchange-inline">in which we continued our previous discussion on fluid flow through the system. Last time</ins>, <ins class="diffchange diffchange-inline">we leaned toward a discrete system because it would cut energy costs due to less activity</ins>. <ins class="diffchange diffchange-inline">However</ins>, <ins class="diffchange diffchange-inline">Dylan told us today that discrete flow would not work because the culture needs to maintain steady state</ins>. <ins class="diffchange diffchange-inline">According to the equations and graphs he drew on the whiteboard, discrete flow would not satisfy this requirement</ins>. <ins class="diffchange diffchange-inline">Any deviation from steady state would add noise to the electrical output from bacteria</ins>, <ins class="diffchange diffchange-inline">rendering the biosensor inaccurate. <p></p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Still, Maneesh and Dylan tried to design a discrete system that would work. A programmable fish food dispenser would periodically release food into the bioreactor that held the bacterial culture. And a microcontroller would be used to activate the water pump. However, the issue of maintaining steady state still lingered. <p></p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">On the whiteboard, we also came up with two other designs--a passive gravity fed system and a continuous model. For continuous flow, pumps would be constantly churning food and water into the bioreactor. The disadvantages are incredibly low flow rates (meaning higher-end machines) and greatest power consumption. <p></p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">In the passive system, food and water input from above the water level would flow down into the bioreactor. This design allowed for continuous flow and least energy consumption. However, the bulk of this system would need to be strictly underwater, so we would have to look into waterproofing</ins>. <ins class="diffchange diffchange-inline"><p></p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">#filtration #flow #gravity</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></div></td></tr>
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</table>DanVLevinehttp://2012.igem.org/wiki/index.php?title=Team:Cornell/testing/notebook/drylab/2&diff=255831&oldid=prevDanVLevine at 07:14, 3 October 20122012-10-03T07:14:33Z<p></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 07:14, 3 October 2012</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h6>Entry:</h6></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h6>Entry:</h6></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <del class="diffchange diffchange-inline">Lorem ipsum dolor sit amet</del>, <del class="diffchange diffchange-inline">consectetur adipisicing elit</del>, <del class="diffchange diffchange-inline">sed do eiusmod tempor incididunt ut labore et dolore magna aliqua</del>. <del class="diffchange diffchange-inline">Ut enim ad minim veniam</del>, <del class="diffchange diffchange-inline">quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat</del>. <del class="diffchange diffchange-inline">Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur</del>. <del class="diffchange diffchange-inline">Excepteur sint occaecat cupidatat non proident</del>, <del class="diffchange diffchange-inline">sunt in culpa qui officia deserunt mollit anim id est laborum</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <ins class="diffchange diffchange-inline">Today was a huge discussion day</ins>, <ins class="diffchange diffchange-inline">in which we continued our previous discussion on fluid flow through the system. Last time</ins>, <ins class="diffchange diffchange-inline">we leaned toward a discrete system because it would cut energy costs due to less activity</ins>. <ins class="diffchange diffchange-inline">However</ins>, <ins class="diffchange diffchange-inline">Dylan told us today that discrete flow would not work because the culture needs to maintain steady state</ins>. <ins class="diffchange diffchange-inline">According to the equations and graphs he drew on the whiteboard, discrete flow would not satisfy this requirement</ins>. <ins class="diffchange diffchange-inline">Any deviation from steady state would add noise to the electrical output from bacteria</ins>, <ins class="diffchange diffchange-inline">rendering the biosensor inaccurate. <p></p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Still, Maneesh and Dylan tried to design a discrete system that would work. A programmable fish food dispenser would periodically release food into the bioreactor that held the bacterial culture. And a microcontroller would be used to activate the water pump. However, the issue of maintaining steady state still lingered. <p></p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">On the whiteboard, we also came up with two other designs--a passive gravity fed system and a continuous model. For continuous flow, pumps would be constantly churning food and water into the bioreactor. The disadvantages are incredibly low flow rates (meaning higher-end machines) and greatest power consumption. <p></p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">In the passive system, food and water input from above the water level would flow down into the bioreactor. This design allowed for continuous flow and least energy consumption. However, the bulk of this system would need to be strictly underwater, so we would have to look into waterproofing</ins>. <ins class="diffchange diffchange-inline"><p></p></ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">#filtration #flow #gravity</ins></div></td></tr>
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</table>DanVLevine