Team:Cornell/testing/notebook/drylab/10

From 2012.igem.org

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<a href="https://2012.igem.org/Team:Cornell/testing/notebook/drylab/pre">Pre</a>
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<a href="https://2012.igem.org/Team:Cornell/testing/notebook/drylab/1">Week 1</a>
<a href="https://2012.igem.org/Team:Cornell/testing/notebook/drylab/1">Week 1</a>
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It has been said that <span class="has-tip" data-width="250" title="Description of what astronomy is.">astronomy</span> is a humbling and character-building experience. There is perhaps no better demonstration of the folly of human conceits than this distant image of our tiny world. To me, it underscores our responsibility to deal more kindly with one another, and to preserve and cherish the pale blue dot, the only home we've ever known. <a href="#" class="technical-desc" for="#technical-desc1" style="display:block;margin-top:20px;">Technical Notes</a>
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It has been said that astronomy is a humbling and character-building experience. There is perhaps no better demonstration of the folly of human conceits than this distant image of our tiny world. To me, it underscores our responsibility to deal more kindly with one another, and to preserve and cherish the pale blue dot, the only home we've ever known.
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Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
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It has been said that astronomy is a humbling and character-building experience. There is perhaps no better demonstration of the folly of human conceits than this distant image of our tiny world. To me, it underscores our responsibility to deal more kindly with one another, and to preserve and cherish the pale blue dot, the only home we've ever known.
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Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
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It has been said that astronomy is a humbling and character-building experience. There is perhaps no better demonstration of the folly of human conceits than this distant image of our tiny world. To me, it underscores our responsibility to deal more kindly with one another, and to preserve and cherish the pale blue dot, the only home we've ever known.
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It has been said that astronomy is a humbling and character-building experience. There is perhaps no better demonstration of the folly of human conceits than this distant image of our tiny world. To me, it underscores our responsibility to deal more kindly with one another, and to preserve and cherish the pale blue dot, the only home we've ever known.
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<h3>Friday, August 10, 2012</h3>
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It has been said that astronomy is a humbling and character-building experience. There is perhaps no better demonstration of the folly of human conceits than this distant image of our tiny world. To me, it underscores our responsibility to deal more kindly with one another, and to preserve and cherish the pale blue dot, the only home we've ever known.
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Focus: Production of More Bioreactors, Further Reactor Tests<p></p>
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<a href="#" class="technical-desc" for="#technical-desc5" style="display:block;margin-top:20px;">Technical Notes</a>
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Problems afleet: pumps are weak and bioreactors leak. Call the police?
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<a href="#" class="technical-desc" for="#technical-desc5" style="display:block;margin-top:20px;">Details</a>
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Manny and I spent the first few days of the week in the Emerson machine shop making caps and bodies for the bioreactors. We successfully press fitted the caps into the clear tube body and tested them for leaks. We screwed the luer locks into the caps after completely filling the reactors with water. We tried various orientations, and found to our satisfaction that everything was tightly sealed. However, when we tried to push water through the bioreactors using the micro-pumps, the pumps failed to overcome the water pressure. Disappointed, we began to look for stronger, more robust pumps to do the job.<p></p>
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We also encountered an issue with preventing leaks from the two graphite rods running through the front cap. To seal the cap holes for the rods, we drilled through the luer locks and ran the rods through after they were fitted with rubber sleeves. Water was able to seep through between the rubber and the plastic luer lock. As a solution, Dan and Manny attempted solder on a thin coating, but when that proved too difficult, melted the rubber and plastic to purge any gaps. Still, water was able to get through. Ultimately, Maneesh’s application of silicone sealant between the luer locks and graphite rods accomplished the objective.  <p></p>
 +
Although there is still a lot to do before the system is ready to be deployed, everybody has worked diligently in their groups this summer. Thus, next week, many of us will be taking off to enjoy what little remains of the summer.<p></p>
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#bioreactor #leaks #vacation
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<h3>Saturday</h3>
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It has been said that astronomy is a humbling and character-building experience. There is perhaps no better demonstration of the folly of human conceits than this distant image of our tiny world. To me, it underscores our responsibility to deal more kindly with one another, and to preserve and cherish the pale blue dot, the only home we've ever known.
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<a href="#" class="technical-desc" for="#technical-desc6" style="display:block;margin-top:20px;">Technical Notes</a>
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Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
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<h3>Friday, August 10, 2012</h3>
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Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
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Manny and I spent the first few days of the week in the Emerson machine shop making caps and bodies for the bioreactors. We successfully press fitted the caps into the clear tube body and tested them for leaks. We screwed the luer locks into the caps after completely filling the reactors with water. We tried various orientations, and found to our satisfaction that everything was tightly sealed. However, when we tried to push water through the bioreactors using the micro-pumps, the pumps failed to overcome the water pressure. Disappointed, we began to look for stronger, more robust pumps to do the job.<p></p>
 +
We also encountered an issue with preventing leaks from the two graphite rods running through the front cap. To seal the cap holes for the rods, we drilled through the luer locks and ran the rods through after they were fitted with rubber sleeves. Water was able to seep through between the rubber and the plastic luer lock. As a solution, Dan and Manny attempted solder on a thin coating, but when that proved too difficult, melted the rubber and plastic to purge any gaps. Still, water was able to get through. Ultimately, Maneesh’s application of silicone sealant between the luer locks and graphite rods accomplished the objective.  <p></p>
 +
Although there is still a lot to do before the system is ready to be deployed, everybody has worked diligently in their groups this summer. Thus, next week, many of us will be taking off to enjoy what little remains of the summer.<p></p>
 +
 
 +
#bioreactor #leaks #vacation
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It has been said that astronomy is a humbling and character-building experience. There is perhaps no better demonstration of the folly of human conceits than this distant image of our tiny world. To me, it underscores our responsibility to deal more kindly with one another, and to preserve and cherish the pale blue dot, the only home we've ever known.
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It has been said that astronomy is a humbling and character-building experience. There is perhaps no better demonstration of the folly of human conceits than this distant image of our tiny world. To me, it underscores our responsibility to deal more kindly with one another, and to preserve and cherish the pale blue dot, the only home we've ever known.
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It has been said that astronomy is a humbling and character-building experience. There is perhaps no better demonstration of the folly of human conceits than this distant image of our tiny world. To me, it underscores our responsibility to deal more kindly with one another, and to preserve and cherish the pale blue dot, the only home we've ever known.
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It has been said that astronomy is a humbling and character-building experience. There is perhaps no better demonstration of the folly of human conceits than this distant image of our tiny world. To me, it underscores our responsibility to deal more kindly with one another, and to preserve and cherish the pale blue dot, the only home we've ever known.
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<h3>Thursday</h3>
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It has been said that astronomy is a humbling and character-building experience. There is perhaps no better demonstration of the folly of human conceits than this distant image of our tiny world. To me, it underscores our responsibility to deal more kindly with one another, and to preserve and cherish the pale blue dot, the only home we've ever known.
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<div class="panel" style="background:white;margin-top: 20px;">
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<h6>Technical Stuff:</h6>
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Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
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<h3>Friday</h3>
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It has been said that astronomy is a humbling and character-building experience. There is perhaps no better demonstration of the folly of human conceits than this distant image of our tiny world. To me, it underscores our responsibility to deal more kindly with one another, and to preserve and cherish the pale blue dot, the only home we've ever known.
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<div class="panel" style="background:white;margin-top: 20px;">
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<h6>Technical Stuff:</h6>
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Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
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<h3>Saturday</h3>
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<h3>Friday, August 10, 2012</h3>
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It has been said that astronomy is a humbling and character-building experience. There is perhaps no better demonstration of the folly of human conceits than this distant image of our tiny world. To me, it underscores our responsibility to deal more kindly with one another, and to preserve and cherish the pale blue dot, the only home we've ever known.
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Focus: Production of More Bioreactors, Further Reactor Tests<p></p>
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Problems afleet: pumps are weak and bioreactors leak. Call the police?
<div class="panel" style="background:white;margin-top: 20px;">
<div class="panel" style="background:white;margin-top: 20px;">
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<h6>Technical Stuff:</h6>
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<h6>Entry:</h6>
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Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
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Manny and I spent the first few days of the week in the Emerson machine shop making caps and bodies for the bioreactors. We successfully press fitted the caps into the clear tube body and tested them for leaks. We screwed the luer locks into the caps after completely filling the reactors with water. We tried various orientations, and found to our satisfaction that everything was tightly sealed. However, when we tried to push water through the bioreactors using the micro-pumps, the pumps failed to overcome the water pressure. Disappointed, we began to look for stronger, more robust pumps to do the job.<p></p>
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We also encountered an issue with preventing leaks from the two graphite rods running through the front cap. To seal the cap holes for the rods, we drilled through the luer locks and ran the rods through after they were fitted with rubber sleeves. Water was able to seep through between the rubber and the plastic luer lock. As a solution, Dan and Manny attempted solder on a thin coating, but when that proved too difficult, melted the rubber and plastic to purge any gaps. Still, water was able to get through. Ultimately, Maneesh’s application of silicone sealant between the luer locks and graphite rods accomplished the objective.  <p></p>
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Although there is still a lot to do before the system is ready to be deployed, everybody has worked diligently in their groups this summer. Thus, next week, many of us will be taking off to enjoy what little remains of the summer.<p></p>
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#bioreactor #leaks #vacation
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<img src="https://static.igem.org/mediawiki/2012/e/e1/Smile_square.jpg">
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Latest revision as of 03:36, 4 October 2012

Progress Log
Details
Both

Week 10

  • Friday, August 10, 2012

    Focus: Production of More Bioreactors, Further Reactor Tests

    Problems afleet: pumps are weak and bioreactors leak. Call the police? Details
    Entry:
    Manny and I spent the first few days of the week in the Emerson machine shop making caps and bodies for the bioreactors. We successfully press fitted the caps into the clear tube body and tested them for leaks. We screwed the luer locks into the caps after completely filling the reactors with water. We tried various orientations, and found to our satisfaction that everything was tightly sealed. However, when we tried to push water through the bioreactors using the micro-pumps, the pumps failed to overcome the water pressure. Disappointed, we began to look for stronger, more robust pumps to do the job.

    We also encountered an issue with preventing leaks from the two graphite rods running through the front cap. To seal the cap holes for the rods, we drilled through the luer locks and ran the rods through after they were fitted with rubber sleeves. Water was able to seep through between the rubber and the plastic luer lock. As a solution, Dan and Manny attempted solder on a thin coating, but when that proved too difficult, melted the rubber and plastic to purge any gaps. Still, water was able to get through. Ultimately, Maneesh’s application of silicone sealant between the luer locks and graphite rods accomplished the objective.

    Although there is still a lot to do before the system is ready to be deployed, everybody has worked diligently in their groups this summer. Thus, next week, many of us will be taking off to enjoy what little remains of the summer.

    #bioreactor #leaks #vacation
  • Friday, August 10, 2012

    Manny and I spent the first few days of the week in the Emerson machine shop making caps and bodies for the bioreactors. We successfully press fitted the caps into the clear tube body and tested them for leaks. We screwed the luer locks into the caps after completely filling the reactors with water. We tried various orientations, and found to our satisfaction that everything was tightly sealed. However, when we tried to push water through the bioreactors using the micro-pumps, the pumps failed to overcome the water pressure. Disappointed, we began to look for stronger, more robust pumps to do the job.

    We also encountered an issue with preventing leaks from the two graphite rods running through the front cap. To seal the cap holes for the rods, we drilled through the luer locks and ran the rods through after they were fitted with rubber sleeves. Water was able to seep through between the rubber and the plastic luer lock. As a solution, Dan and Manny attempted solder on a thin coating, but when that proved too difficult, melted the rubber and plastic to purge any gaps. Still, water was able to get through. Ultimately, Maneesh’s application of silicone sealant between the luer locks and graphite rods accomplished the objective.

    Although there is still a lot to do before the system is ready to be deployed, everybody has worked diligently in their groups this summer. Thus, next week, many of us will be taking off to enjoy what little remains of the summer.

    #bioreactor #leaks #vacation
  • Friday, August 10, 2012

    Focus: Production of More Bioreactors, Further Reactor Tests

    Problems afleet: pumps are weak and bioreactors leak. Call the police?
    Entry:
    Manny and I spent the first few days of the week in the Emerson machine shop making caps and bodies for the bioreactors. We successfully press fitted the caps into the clear tube body and tested them for leaks. We screwed the luer locks into the caps after completely filling the reactors with water. We tried various orientations, and found to our satisfaction that everything was tightly sealed. However, when we tried to push water through the bioreactors using the micro-pumps, the pumps failed to overcome the water pressure. Disappointed, we began to look for stronger, more robust pumps to do the job.

    We also encountered an issue with preventing leaks from the two graphite rods running through the front cap. To seal the cap holes for the rods, we drilled through the luer locks and ran the rods through after they were fitted with rubber sleeves. Water was able to seep through between the rubber and the plastic luer lock. As a solution, Dan and Manny attempted solder on a thin coating, but when that proved too difficult, melted the rubber and plastic to purge any gaps. Still, water was able to get through. Ultimately, Maneesh’s application of silicone sealant between the luer locks and graphite rods accomplished the objective.

    Although there is still a lot to do before the system is ready to be deployed, everybody has worked diligently in their groups this summer. Thus, next week, many of us will be taking off to enjoy what little remains of the summer.

    #bioreactor #leaks #vacation