Team:Cornell/Sandbox/notebook
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<a href="#May">May</a> | <a href="#May">May</a> | ||
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+ | <div id="WikiTest" class="row entry wikinb"> | ||
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<div id="April" class="row entry wetnb"> | <div id="April" class="row entry wetnb"> | ||
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<h3>April</h3> | <h3>April</h3> | ||
From June 12th-22nd, the DeLisa lab kindly hosted a synthetic biology ‘bootcamp’ for our team members. Dr. Didi Waraho assisted in the instruction of new team members, while Taylor Stevenson helped more experienced members get acquainted with the Gibson assembly method. | From June 12th-22nd, the DeLisa lab kindly hosted a synthetic biology ‘bootcamp’ for our team members. Dr. Didi Waraho assisted in the instruction of new team members, while Taylor Stevenson helped more experienced members get acquainted with the Gibson assembly method. | ||
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<h3>May</h3> | <h3>May</h3> | ||
We have successfully created working arsenic and salicylate sensing constructs and have shown that they are functional in Shewanella and are continuing to characterization of our arsenic and salicylate constructs. Although the response in our reactors is not as sensitive as we would hope, we have various hypotheses that we hope to test. Furthermore, we are using RT-qPCR, western blots, and fluorescence measurements to quantify transcription and translation of mtrB levels in response to varying concentrations of arsenic and salicylate. We also plan on running time courses to determine when mtrB is expressed in the presence of analyte. | We have successfully created working arsenic and salicylate sensing constructs and have shown that they are functional in Shewanella and are continuing to characterization of our arsenic and salicylate constructs. Although the response in our reactors is not as sensitive as we would hope, we have various hypotheses that we hope to test. Furthermore, we are using RT-qPCR, western blots, and fluorescence measurements to quantify transcription and translation of mtrB levels in response to varying concentrations of arsenic and salicylate. We also plan on running time courses to determine when mtrB is expressed in the presence of analyte. |
Revision as of 20:15, 5 March 2013
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