Team:Cornell/Notebook/Salicylate reporter

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June

Week

June 29th, Friday

  • Vent PCR at 11:00 (DPW)
    • Dylan's magic triple anneal method (55/60/63)
  • Gel purified PCR product (DPW)
    • Quantified product at 22.4 ng/uL
    • Set up digestion of PCR product with EcoRI-HF and AscI.
      • 22 ng/uL --> 45.5 uL sample for 1 ug digest
      • Used 5.3 uL NEB Buffer 4
      • 1 uL of each enzyme, diluted to 1 unit/uL
  • Miniprepped overnight cultures of PL14-PL20 (STC)
    • Using C1015 rotor, 6666 x g, the Corning culture tubes only fit in the centrifuge with the lids off
  • Made LB, 3x 60 mL in 100 mL bottles (SS)
  • Made LB Agar, 4 x 250 mL LB Agar in 500 mL bottles (CS)
  • Autoclaved LB, LB Agar, and milliQ (SS)
  • Made LB plates with Kan (CMR)