Team:Cornell/Notebook/Salicylate reporter

From 2012.igem.org

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===[[Team:Cornell/Notebook/Salicylate_reporter/July1|July 1st-7th]]===
===[[Team:Cornell/Notebook/Salicylate_reporter/July1|July 1st-7th]]===
===[[Team:Cornell/Notebook/Salicylate_reporter/July8|July 8th-14th]]===
===[[Team:Cornell/Notebook/Salicylate_reporter/July8|July 8th-14th]]===
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Today we set up conjugations between our new donor E. coli and S. Oneidensis.
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[[Image:2012_7_13_DylanConjugating.jpg|thumb|right|Dylan plating putatively promiscuous E. coli mixed with S. Oneidensis.]]
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===[[Team:Cornell/Notebook/Salicylate_reporter/July15|July 15th-21st]]===
===[[Team:Cornell/Notebook/Salicylate_reporter/July15|July 15th-21st]]===

Revision as of 13:51, 13 July 2012

Home Team Project Parts Modeling Notebook Protocols Safety Attributions

Contents

The salicylate reporter is analogous to the arsenic reporter, but with a salicylate-sensitive promoter. That is, in the presence of salicylate it will produce MtrB, completing the Mtr pathway and allowing Shewanella to produce current in our biosensor. In combination with the nah operon, this reporter will be able to detect naphthalene levels.

June

June 24th-30th

July

July 1st-7th

July 8th-14th

Today we set up conjugations between our new donor E. coli and S. Oneidensis.

Dylan plating putatively promiscuous E. coli mixed with S. Oneidensis.

===July 15th-21st===