Team:Cornell/Notebook/Salicylate reporter

From 2012.igem.org

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Write description
 
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==June==
 
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===Week===
 
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====June 29th, Friday====
 
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* [[Vent PCR]] at 11:00 (DPW)
 
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** Dylan's magic triple anneal method (55/60/63)
 
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* Gel purified PCR product (DPW)
 
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** Quantified product at  22.4 ng/uL
 
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** Set up digestion of PCR product with EcoRI-HF and AscI.
 
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*** 22 ng/uL --> 45.5 uL sample for 1 ug digest
 
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*** Buffer 4
 
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** Ran digestion on gel.
 
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* [[Miniprep]]ped overnight cultures of PL14-PL20 (STC)
 
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** Using C1015 rotor, 6666 x g, the Corning culture tubes only fit in the centrifuge with the lids off
 
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* Made [[LB]], 3x 60 mL in 100 mL bottles (SS)
 
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* Made [[LB|LB Agar]], 4 x 250 mL LB Agar in 500 mL bottles (CS)
 
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* [[Autoclave]]d LB, LB Agar, and milliQ (SS)
 
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* Made [[LB]] plates with Kan (CMR)
 
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* CUGEM movie outing at 8:00 pm.
 
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* [[Phusion PCR]] at 10:00 pm (DPW)
 
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** Dylan's magic triple anneal method (57/65/70, 35 cycles total)
 
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** Amplifying nah operon from Gibson 1
 
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** Appending BioBrick cutsites for ligation into pSB3C5
 

Latest revision as of 16:24, 26 October 2012