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Team:Cornell/Notebook/Salicylate reporter
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===Week=== | ===Week=== | ||
====June 29th, Friday==== | ====June 29th, Friday==== | ||
| - | * Began preparation for [[Vent PCR]](DPW) | + | * Began preparation for [[Vent PCR]](DPW) at 10:56 AM |
** Dylan's magic triple anneal method (55/60/63). | ** Dylan's magic triple anneal method (55/60/63). | ||
* Thermal cycler started at 11:00 am. | * Thermal cycler started at 11:00 am. | ||
* Gel purified PCR product. | * Gel purified PCR product. | ||
| + | * [[Miniprep]]ped (STC) overnight cultures of PL0014-PL0020 | ||
| + | ** Using C1015 rotor, 6666 x g, the Corning culture tubes only fit in the centrifuge with the lids off | ||
| + | * Made [[LB]], 3x 60 mL in 100 mL bottles (SS) | ||
| + | * Made [[LB|LB Agar]], 4 x 250 mL LB Agar in 500 mL bottles (CS) | ||
| + | * [[Autoclave]]d LB, LB Agar, and milliQ (ss) | ||
Revision as of 18:55, 29 June 2012
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June
Week
June 29th, Friday
- Began preparation for Vent PCR(DPW) at 10:56 AM
- Dylan's magic triple anneal method (55/60/63).
- Thermal cycler started at 11:00 am.
- Gel purified PCR product.
- Miniprepped (STC) overnight cultures of PL0014-PL0020
- Using C1015 rotor, 6666 x g, the Corning culture tubes only fit in the centrifuge with the lids off
- Made LB, 3x 60 mL in 100 mL bottles (SS)
- Made LB Agar, 4 x 250 mL LB Agar in 500 mL bottles (CS)
- Autoclaved LB, LB Agar, and milliQ (ss)
