http://2012.igem.org/wiki/index.php?title=Team:Cornell/Notebook/Nah_operon/June17&feed=atom&action=historyTeam:Cornell/Notebook/Nah operon/June17 - Revision history2024-03-29T00:12:33ZRevision history for this page on the wikiMediaWiki 1.16.0http://2012.igem.org/wiki/index.php?title=Team:Cornell/Notebook/Nah_operon/June17&diff=24114&oldid=prevCpaduano: /* June 19th, Tuesday */2012-07-03T21:56:57Z<p><span class="autocomment">June 19th, Tuesday</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==June 19th, Tuesday==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==June 19th, Tuesday==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Ran [[Team:Cornell/Notebook/Gibson_assembly|Gibson Assembly]] of nah operon fragments into pBMT-1 backbone and [[Team:Cornell/Notebook/Transformation_ecoli | transformed]] into DH5a electrocompotent ''E. coli'' cells. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Ran [[Team:Cornell/Notebook/Gibson_assembly|Gibson Assembly]] of nah operon fragments into pBMT-1 backbone and [[Team:Cornell/Notebook/Transformation_ecoli | transformed]] into DH5a electrocompotent ''E. coli'' cells. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*Set up [[Team:Cornell/Notebook/<del class="diffchange diffchange-inline">PCR</del>|PCR]] to amplify the Gibson Assembly products. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*Set up [[Team:Cornell/Notebook/<ins class="diffchange diffchange-inline">Phusion_PCR</ins>|PCR]] to amplify the Gibson Assembly products. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**''Work done by: Dylan and Swati''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**''Work done by: Dylan and Swati''</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>Cpaduanohttp://2012.igem.org/wiki/index.php?title=Team:Cornell/Notebook/Nah_operon/June17&diff=24103&oldid=prevCpaduano at 21:52, 3 July 20122012-07-03T21:52:14Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Team:Cornell/Notebook/Nah_operon|Back to nah operon week view]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Team:Cornell/Notebook/Nah_operon|Back to nah operon week view]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">==</del>=June 17th-23rd<del class="diffchange diffchange-inline">==</del>=</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>=June 17th-23rd=</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">==</del>==June 17th, Sunday<del class="diffchange diffchange-inline">==</del>==</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>==June 17th, Sunday==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Successful PCR of pBMT-1 [[Team:Cornell/Notebook/Gel_purification| gel purified]].</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Successful PCR of pBMT-1 [[Team:Cornell/Notebook/Gel_purification| gel purified]].</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">==</del>==June 19th, Tuesday<del class="diffchange diffchange-inline">==</del>==</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>==June 19th, Tuesday==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Ran [[Team:Cornell/Notebook/Gibson_assembly|Gibson Assembly]] of nah operon fragments into pBMT-1 backbone and [[Team:Cornell/Notebook/Transformation_ecoli | transformed]] into DH5a electrocompotent ''E. coli'' cells. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Ran [[Team:Cornell/Notebook/Gibson_assembly|Gibson Assembly]] of nah operon fragments into pBMT-1 backbone and [[Team:Cornell/Notebook/Transformation_ecoli | transformed]] into DH5a electrocompotent ''E. coli'' cells. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Set up [[Team:Cornell/Notebook/PCR|PCR]] to amplify the Gibson Assembly products. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Set up [[Team:Cornell/Notebook/PCR|PCR]] to amplify the Gibson Assembly products. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**''Work done by: Dylan and Swati''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**''Work done by: Dylan and Swati''</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">==</del>==June 20th, Wednesday<del class="diffchange diffchange-inline">==</del>==</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>==June 20th, Wednesday==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Set up a [[Team:Cornell/Notebook/Digestion|digestion]] of the PCR of Gibson Assembly products and ran the digested products on a gel to see if Gibson worked.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Set up a [[Team:Cornell/Notebook/Digestion|digestion]] of the PCR of Gibson Assembly products and ran the digested products on a gel to see if Gibson worked.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Three colonies on plates of DH5a transformed with Gibson Assembly product. Made liquid cultures to miniprep and sequence.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Three colonies on plates of DH5a transformed with Gibson Assembly product. Made liquid cultures to miniprep and sequence.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**''Work done by: Dylan and Swati''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**''Work done by: Dylan and Swati''</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">==</del>==June 22nd, Friday<del class="diffchange diffchange-inline">==</del>==</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>==June 22nd, Friday==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Cornell/Notebook/Miniprep | Miniprepped]] directly transformed Gibson Assembly product for sequencing using the the Gibson nah1F and nah4R primers (each w/ 20 bp overhangs).</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Cornell/Notebook/Miniprep | Miniprepped]] directly transformed Gibson Assembly product for sequencing using the the Gibson nah1F and nah4R primers (each w/ 20 bp overhangs).</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Ran undigested miniprep with [[Team:Cornell/Notebook/Gel_electrophoresis|gel electrophoresis]], looking for large bands corresponding to supercoiled DNA. The gel shows distinct bands for all three lanes. We interpreted this to mean that we got product. Submitted for [[Team:Cornell/Notebook/Sequencing|sequencing]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Ran undigested miniprep with [[Team:Cornell/Notebook/Gel_electrophoresis|gel electrophoresis]], looking for large bands corresponding to supercoiled DNA. The gel shows distinct bands for all three lanes. We interpreted this to mean that we got product. Submitted for [[Team:Cornell/Notebook/Sequencing|sequencing]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::[[File:Cornell2012_0621_Gibson-entire-plasmid.jpg|600px]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::[[File:Cornell2012_0621_Gibson-entire-plasmid.jpg|600px]]</div></td></tr>
</table>Cpaduanohttp://2012.igem.org/wiki/index.php?title=Team:Cornell/Notebook/Nah_operon/June17&diff=24069&oldid=prevCpaduano: /* June 20th, Wednesday */2012-07-03T21:37:51Z<p><span class="autocomment">June 20th, Wednesday</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====June 20th, Wednesday====</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====June 20th, Wednesday====</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Set up a [[Team:Cornell/Notebook/Digestion|digestion]] of the PCR of Gibson Assembly products and ran the digested products on a gel to see if Gibson worked.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Set up a [[Team:Cornell/Notebook/Digestion|digestion]] of the PCR of Gibson Assembly products and ran the digested products on a gel to see if Gibson worked.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*Three colonies on plates of DH5a transformed with Gibson Assembly product. Made <del class="diffchange diffchange-inline">[[</del>liquid cultures<del class="diffchange diffchange-inline">]] </del>to miniprep and sequence.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*Three colonies on plates of DH5a transformed with Gibson Assembly product. Made liquid cultures to miniprep and sequence.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**''Work done by: Dylan and Swati''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**''Work done by: Dylan and Swati''</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>Cpaduanohttp://2012.igem.org/wiki/index.php?title=Team:Cornell/Notebook/Nah_operon/June17&diff=24068&oldid=prevCpaduano: /* June 17th-23rd */2012-07-03T21:37:18Z<p><span class="autocomment">June 17th-23rd</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===June 17th-23rd===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===June 17th-23rd===</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====June 17th, Sunday====</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====June 17th, Sunday====</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*Successful PCR of pBMT-1 [[<del class="diffchange diffchange-inline">Gel Purification </del>| gel purified]].</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*Successful PCR of pBMT-1 [[<ins class="diffchange diffchange-inline">Team:Cornell/Notebook/Gel_purification</ins>| gel purified]].</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====June 19th, Tuesday====</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====June 19th, Tuesday====</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*Ran [[Gibson Assembly]] of nah operon fragments into pBMT-1 backbone and [[<del class="diffchange diffchange-inline">Transformation </del>| transformed]] into DH5a electrocompotent ''E. coli'' cells. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*Ran [[<ins class="diffchange diffchange-inline">Team:Cornell/Notebook/Gibson_assembly|</ins>Gibson Assembly]] of nah operon fragments into pBMT-1 backbone and [[<ins class="diffchange diffchange-inline">Team:Cornell/Notebook/Transformation_ecoli </ins>| transformed]] into DH5a electrocompotent ''E. coli'' cells. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*Set up [[PCR]] to amplify the Gibson Assembly products. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*Set up [[<ins class="diffchange diffchange-inline">Team:Cornell/Notebook/PCR|</ins>PCR]] to amplify the Gibson Assembly products. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**''Work done by: Dylan and Swati''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**''Work done by: Dylan and Swati''</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====June 20th, Wednesday====</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====June 20th, Wednesday====</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*Set up a [[digestion]] of the PCR of Gibson Assembly products and ran the digested products on a gel to see if Gibson worked.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*Set up a [[<ins class="diffchange diffchange-inline">Team:Cornell/Notebook/Digestion|</ins>digestion]] of the PCR of Gibson Assembly products and ran the digested products on a gel to see if Gibson worked.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Three colonies on plates of DH5a transformed with Gibson Assembly product. Made [[liquid cultures]] to miniprep and sequence.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Three colonies on plates of DH5a transformed with Gibson Assembly product. Made [[liquid cultures]] to miniprep and sequence.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**''Work done by: Dylan and Swati''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**''Work done by: Dylan and Swati''</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====June 22nd, Friday====</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====June 22nd, Friday====</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*[[Miniprep | Miniprepped]] directly transformed Gibson Assembly product for sequencing using the the Gibson nah1F and nah4R primers (each w/ 20 bp overhangs).</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*[[<ins class="diffchange diffchange-inline">Team:Cornell/Notebook/</ins>Miniprep | Miniprepped]] directly transformed Gibson Assembly product for sequencing using the the Gibson nah1F and nah4R primers (each w/ 20 bp overhangs).</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*Ran undigested miniprep with [<del class="diffchange diffchange-inline">https://2011.igem.org/</del>Team:Cornell/<del class="diffchange diffchange-inline">Protocols </del>gel electrophoresis], looking for large bands corresponding to supercoiled DNA. The gel shows distinct bands for all three lanes. We interpreted this to mean that we got product. Submitted for [[sequencing]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*Ran undigested miniprep with [<ins class="diffchange diffchange-inline">[</ins>Team:Cornell/<ins class="diffchange diffchange-inline">Notebook/Gel_electrophoresis|</ins>gel electrophoresis<ins class="diffchange diffchange-inline">]</ins>], looking for large bands corresponding to supercoiled DNA. The gel shows distinct bands for all three lanes. We interpreted this to mean that we got product. Submitted for [[<ins class="diffchange diffchange-inline">Team:Cornell/Notebook/Sequencing|</ins>sequencing]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::[[File:Cornell2012_0621_Gibson-entire-plasmid.jpg|600px]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::[[File:Cornell2012_0621_Gibson-entire-plasmid.jpg|600px]]</div></td></tr>
</table>Cpaduanohttp://2012.igem.org/wiki/index.php?title=Team:Cornell/Notebook/Nah_operon/June17&diff=24024&oldid=prevCpaduano at 21:14, 3 July 20122012-07-03T21:14:06Z<p></p>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">{{:Team:Cornell/Navbar}}</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">{{:Team:Cornell/TOC|right}}</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">The purpose of this subproject is to make the nah operon biobrick compatible for the use of future iGEM teams. The nah operon codes for a pathway that converts naphthalene into salicylate. In our system, this will allow naphthalene to be detected by the salicylate reporter. There are three standard cut sites internal to the operon which we are removing - PstI, and two NotI sites. This biobrick compatible piece could be useful to future teams in projects that involve bioremediation or detection of naphthalene.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[Team:Cornell/Notebook/Nah_operon|Back to nah operon week view]]</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===June 17th-23rd===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===June 17th-23rd===</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====June 17th, Sunday====</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====June 17th, Sunday====</div></td></tr>
</table>Cpaduanohttp://2012.igem.org/wiki/index.php?title=Team:Cornell/Notebook/Nah_operon/June17&diff=24023&oldid=prevCpaduano: Created page with "===June 17th-23rd=== ====June 17th, Sunday==== *Successful PCR of pBMT-1 gel purified. ====June 19th, Tuesday==== *Ran Gibson Assembly of nah operon f..."2012-07-03T21:13:39Z<p>Created page with "===June 17th-23rd=== ====June 17th, Sunday==== *Successful PCR of pBMT-1 <a href="/wiki/index.php?title=Gel_Purification&action=edit&redlink=1" class="new" title="Gel Purification (page does not exist)"> gel purified</a>. ====June 19th, Tuesday==== *Ran <a href="/wiki/index.php?title=Gibson_Assembly&action=edit&redlink=1" class="new" title="Gibson Assembly (page does not exist)">Gibson Assembly</a> of nah operon f..."</p>
<p><b>New page</b></p><div>===June 17th-23rd===<br />
====June 17th, Sunday====<br />
*Successful PCR of pBMT-1 [[Gel Purification | gel purified]].<br />
<br />
====June 19th, Tuesday====<br />
*Ran [[Gibson Assembly]] of nah operon fragments into pBMT-1 backbone and [[Transformation | transformed]] into DH5a electrocompotent ''E. coli'' cells. <br />
*Set up [[PCR]] to amplify the Gibson Assembly products. <br />
**''Work done by: Dylan and Swati''<br />
<br />
====June 20th, Wednesday====<br />
*Set up a [[digestion]] of the PCR of Gibson Assembly products and ran the digested products on a gel to see if Gibson worked.<br />
*Three colonies on plates of DH5a transformed with Gibson Assembly product. Made [[liquid cultures]] to miniprep and sequence.<br />
**''Work done by: Dylan and Swati''<br />
<br />
====June 22nd, Friday====<br />
*[[Miniprep | Miniprepped]] directly transformed Gibson Assembly product for sequencing using the the Gibson nah1F and nah4R primers (each w/ 20 bp overhangs).<br />
*Ran undigested miniprep with [https://2011.igem.org/Team:Cornell/Protocols gel electrophoresis], looking for large bands corresponding to supercoiled DNA. The gel shows distinct bands for all three lanes. We interpreted this to mean that we got product. Submitted for [[sequencing]]<br />
::[[File:Cornell2012_0621_Gibson-entire-plasmid.jpg|600px]]</div>Cpaduano