Team:Cornell/Notebook/Arsenic reporter/June10

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===June 10th-16th===
 
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====June 13th, Wednesday====
 
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*Set up [[PCR]] for four fragments of the nah operon as well as the pBMT-1 backbone, using primers designed to mutate cut-sites concurrently with Gibson Assembly. Also set up a PCR for the entire nah operon. Due to length of the fragments, a longer extension time was chosen.
 
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*If Gibson Assembly fails, but we are able to PCR the entire 10kb nah operon using Phusion polymerase, an alternate method of mutation using Phusion will be pursued.
 
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**''Work done by: Caleb, Claire, Dylan, Spencer, Steven, and Swati''
 
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====June 14th, Thursday====
 
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*PCR only amplified nah1, nah3, and nah4 - longer fragments (nah2, the full nah operon, and pBMT-1) were not identified on the PCR product gel. Set up another [[PCR]] with shorter extension time.
 
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====June 15th, Friday====
 
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*PCR of four out of five products visible on gel. Set up PCR of pBMT-1, final fragment required for Gibson Assembly of the nah operon.
 

Latest revision as of 19:49, 19 July 2012