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Protocol for Creating Plates

  • Prepare 1L bottle
  • For 500mL, add 7.5g of agar and 12.5g of LB
  • Pour deionized water until water level reaches 500mL
  • Cover bottle with foil and tape with autoclave tape
  • Autoclave on slow for 20 minutes
  • After autoclaving, let bottle stand cool until touch
  • Apply 500µl of 1000x stock antibiotics to make 1x solution
  • Pour solution into plates