Team:Columbia-Cooper-NYC/Plates

From 2012.igem.org

(Difference between revisions)
(Created page with "= Protocol for Creating Plates = * Prepare 1L bottle * For 500mL, add 7.5g of agar and 12.5g of LB * Pour deionized water until water level reaches 500mL * Cover bottle with foil...")
Line 1: Line 1:
 +
<html>
 +
<head>
 +
<style type="text/css">
 +
body{background-image:url('http://farm9.staticflickr.com/8039/7990439399_1970f791f6_h.jpg');
 +
background-position:top left;
 +
background-repeat:repeat-y
 +
 +
body{background-image:url('http://farm9.staticflickr.com/8173/7989724336_9e5441e3ff_b.jpg');
 +
background-position:top right;
 +
background-repeat:repeat-y
 +
}
 +
 +
 +
 +
</style>
 +
</head>
 +
</html>
 +
 +
 +
 +
<html>
 +
<head>
 +
<body>
 +
<!-- dd menu -->
 +
<script type="text/javascript">
 +
<!--
 +
var timeout        = 500;
 +
var closetimer = 0;
 +
var ddmenuitem      = 0;
 +
 +
// open hidden layer
 +
function mopen(id)
 +
{
 +
// cancel close timer
 +
mcancelclosetime();
 +
 +
// close old layer
 +
if(ddmenuitem) ddmenuitem.style.visibility = 'hidden';
 +
 +
// get new layer and show it
 +
ddmenuitem = document.getElementById(id);
 +
ddmenuitem.style.visibility = 'visible';
 +
 +
}
 +
// close showed layer
 +
function mclose()
 +
{
 +
if(ddmenuitem) ddmenuitem.style.visibility = 'hidden';
 +
}
 +
 +
// go close timer
 +
function mclosetime()
 +
{
 +
closetimer = window.setTimeout(mclose, timeout);
 +
}
 +
 +
// cancel close timer
 +
function mcancelclosetime()
 +
{
 +
if(closetimer)
 +
{
 +
window.clearTimeout(closetimer);
 +
closetimer = null;
 +
}
 +
}
 +
 +
// close layer when click-out
 +
document.onclick = mclose;
 +
// -->
 +
</script>
 +
<style>
 +
#sddm
 +
{ margin: 0;
 +
padding: 0;
 +
      z-index: 30}
 +
 +
#sddm li
 +
{ margin: 0;
 +
padding: 0;
 +
list-style: none;
 +
float: left;
 +
font: bold 11px arial}
 +
 +
#sddm li a
 +
{ display: block;
 +
margin: 0 1px 0 0;
 +
padding: 4px 10px;
 +
width: 85px;
 +
background: #238E23;
 +
color: #FFF;
 +
text-align: center;
 +
text-decoration: none}
 +
 +
#sddm li a:hover
 +
{ background: #B87333}
 +
 +
#sddm div
 +
{ position: absolute;
 +
visibility: hidden;
 +
margin: 0;
 +
padding: 0;
 +
background: #4CC552;
 +
border: 1px solid #5C3317}
 +
 +
#sddm div a
 +
{ position: relative;
 +
display: block;
 +
margin: 0;
 +
padding: 5px 10px;
 +
width: auto;
 +
white-space: nowrap;
 +
text-align: left;
 +
text-decoration: none;
 +
background: #B5EAAA;
 +
color: #5C3317;
 +
font: 11px arial}
 +
 +
#sddm div a:hover
 +
{ background: #49A3FF;
 +
color: #FFF}
 +
</style>
 +
 +
 +
<!-- div class="sample" style="margin-bottom: 15px;height:42px;"><span -->
 +
<ul id="sddm" style="width:1000px;margin:0 auto">
 +
<li><a href="http://2012.igem.org/Team:Columbia-Cooper-NYC" onmouseover="mopen('m1')" onmouseout="mclosetime()">Home</a>
 +
<div id="m1" onmouseover="mcancelclosetime()" onmouseout="mclosetime()">
 +
</div>
 +
</li>
 +
<li><a href="#" onmouseover="mopen('m2')" onmouseout="mclosetime()">Team</a>
 +
<div id="m2" onmouseover="mcancelclosetime()" onmouseout="mclosetime()">
 +
<a href="http://2012.igem.org/Team:Columbia-Cooper-NYC/Team">Biographies</a>
 +
<a href="http://2012.igem.org/Team:Columbia-Cooper-NYC/Facilitators">Facilitators</a>
 +
<a href="http://2012.igem.org/Team:Columbia-Cooper-NYC/Universities">The University(s)</a>
 +
<a href="http://igem.org/Team.cgi?year=2012">Official Profile</a>
 +
</div>
 +
</li>
 +
<li><a href="#" onmouseover="mopen('m3')" onmouseout="mclosetime()">Project</a>
 +
<div id="m3" onmouseover="mcancelclosetime()" onmouseout="mclosetime()">
 +
<a href="http://2012.igem.org/Team:Columbia-Cooper-NYC/Overview">Overview</a>
 +
                <a href="http://2012.igem.org/Team:Columbia-Cooper-NYC/Data_and_Conclusions">Data and Conclusions</a>
 +
                <a href="http://2012.igem.org/Team:Columbia-Cooper-NYC/Future_Directions">Future Directions</a>
 +
                <a href="http://2012.igem.org/Team:Columbia-Cooper-NYC/References">References</a>
 +
</div>
 +
</li>
 +
<li><a href="#" onmouseover="mopen('m4')" onmouseout="mclosetime()">Parts</a>
 +
<div id="m4" onmouseover="mcancelclosetime()" onmouseout="mclosetime()">
 +
<a href="http://2012.igem.org/Team:Columbia-Cooper-NYC/Parts_Submitted">Parts Submitted</a>
 +
</div>
 +
</li>
 +
<li><a href="#" onmouseover="mopen('m5')" onmouseout="mclosetime()">Lab Notebook</a>
 +
<div id="m5" onmouseover="mcancelclosetime()" onmouseout="mclosetime()">
 +
<a href="http://2012.igem.org/Team:Columbia-Cooper-NYC/Columbia_notebook_2">Genetics</a>
 +
<a href="http://2012.igem.org/Team:Columbia-Cooper-NYC/Columbia_notebook_1">Copper Etching</a>
 +
<a href="http://2012.igem.org/Team:Columbia-Cooper-NYC/Protocols">Protocols</a>
 +
</div>
 +
</li>
 +
        <li><a href="#" onmouseover="mopen('m6')" onmouseout="mclosetime()">Human Practice</a>
 +
<div id="m6" onmouseover="mcancelclosetime()" onmouseout="mclosetime()">
 +
<a href="http://2012.igem.org/Team:Columbia-Cooper-NYC/Outreach">Outreach</a>
 +
<a href="http://2012.igem.org/Team:Columbia-Cooper-NYC/Collaborations">Collaboration</a>
 +
</div>
 +
</li>
 +
        <li><a href="http://2012.igem.org/Team:Columbia-Cooper-NYC/Safety" onmouseover="mopen('m7')" onmouseout="mclosetime()">Safety</a>
 +
<div id="m7" onmouseover="mcancelclosetime()" onmouseout="mclosetime()">
 +
                <a href="http://2012.igem.org/Team:Columbia-Cooper-NYC/Safety">Safety</a>
 +
                <a href="http://2012.igem.org/Security">Security</a>
 +
</div>
 +
</li>
 +
        <li><a href="#" onmouseover="mopen('m8')" onmouseout="mclosetime()">Sponsors</a>
 +
<div id="m8" onmouseover="mcancelclosetime()" onmouseout="mclosetime()">
 +
                <a href="http://2012.igem.org/Team:Columbia-Cooper-NYC/Sponsor_Us">Sponsor Us</a>
 +
<a href="http://2012.igem.org/Team:Columbia-Cooper-NYC/Acknowledgements">Acknowledgements</a>
 +
</div>
 +
</li>
 +
        <li><a href="http://2012.igem.org/Main_Page" onmouseover="mopen('m9')" onmouseout="mclosetime()">iGEM</a>
 +
<div id="m9" onmouseover="mcancelclosetime()" onmouseout="mclosetime()">
 +
</div>
 +
</li>
 +
</ul>
 +
<div style="clear:both"></div>
 +
<!-- /span></div -->
 +
<!-- /dd -->
 +
</script>
 +
</body>
 +
</head>
 +
</html>
 +
= Protocol for Creating Plates =
= Protocol for Creating Plates =
-
* Prepare 1L bottle
+
* The following is the protocol for creating plates to grow bacteria on:
-
* For 500mL, add 7.5g of agar and 12.5g of LB
+
# Prepare 1L bottle
-
* Pour deionized water until water level reaches 500mL
+
# For 500mL, add 7.5g of agar and 12.5g of LB
-
* Cover bottle with foil and tape with autoclave tape
+
# Pour deionized water until water level reaches 500mL
-
* Autoclave on slow for 20 minutes
+
# Cover bottle with foil and tape with autoclave tape
-
* After autoclaving, let bottle stand cool until touch
+
# Autoclave on slow for 20 minutes
-
* Apply 500µl of 1000x stock antibiotics to make 1x solution
+
# After autoclaving, let bottle stand cool until touch
-
* Pour solution into plates
+
# Apply 500µl of 1000x stock antibiotics to make 1x solution
 +
# Pour solution into plates

Revision as of 02:23, 3 October 2012


Protocol for Creating Plates

  • The following is the protocol for creating plates to grow bacteria on:
  1. Prepare 1L bottle
  2. For 500mL, add 7.5g of agar and 12.5g of LB
  3. Pour deionized water until water level reaches 500mL
  4. Cover bottle with foil and tape with autoclave tape
  5. Autoclave on slow for 20 minutes
  6. After autoclaving, let bottle stand cool until touch
  7. Apply 500µl of 1000x stock antibiotics to make 1x solution
  8. Pour solution into plates