Team:Columbia-Cooper-NYC/Plates

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(Created page with "= Protocol for Creating Plates = * Prepare 1L bottle * For 500mL, add 7.5g of agar and 12.5g of LB * Pour deionized water until water level reaches 500mL * Cover bottle with foil...")
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= Protocol for Creating Plates =
= Protocol for Creating Plates =
-
* Prepare 1L bottle
+
* The following is the protocol for creating plates to grow bacteria on:
-
* For 500mL, add 7.5g of agar and 12.5g of LB
+
# Prepare 1L bottle
-
* Pour deionized water until water level reaches 500mL
+
# For 500mL, add 7.5g of agar and 12.5g of LB
-
* Cover bottle with foil and tape with autoclave tape
+
# Pour deionized water until water level reaches 500mL
-
* Autoclave on slow for 20 minutes
+
# Cover bottle with foil and tape with autoclave tape
-
* After autoclaving, let bottle stand cool until touch
+
# Autoclave on slow for 20 minutes
-
* Apply 500µl of 1000x stock antibiotics to make 1x solution
+
# After autoclaving, let bottle stand cool until touch
-
* Pour solution into plates
+
# Apply 500µl of 1000x stock antibiotics to make 1x solution
 +
# Pour solution into plates

Revision as of 02:23, 3 October 2012


Protocol for Creating Plates

  • The following is the protocol for creating plates to grow bacteria on:
  1. Prepare 1L bottle
  2. For 500mL, add 7.5g of agar and 12.5g of LB
  3. Pour deionized water until water level reaches 500mL
  4. Cover bottle with foil and tape with autoclave tape
  5. Autoclave on slow for 20 minutes
  6. After autoclaving, let bottle stand cool until touch
  7. Apply 500µl of 1000x stock antibiotics to make 1x solution
  8. Pour solution into plates