Team:Columbia-Cooper-NYC/OD Measurement


Revision as of 02:19, 3 October 2012 by Ymakita (Talk | contribs)

Optical Density Measurement Protocol

  1. Preset temperature and wavelength for measuring the optical density
  2. Read and reference 1mL control solution (e.g. if E. coli was grown in LB/CAM) placed in disposable cuvette
  3. Place 10µl cells into 1mL solution
  4. Read the absorbance

Make note that when reading the optical density, must be referenced by a reference solution

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