Team:Columbia-Cooper-NYC/OD Measurement

From 2012.igem.org

(Difference between revisions)
(Created page with "{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center" !align="center"|[[Team:Columbia-Cooper-NY...")
Line 13: Line 13:
= Optical Density Measurement Protocol =
= Optical Density Measurement Protocol =
-
* Preset temperature and wavelength for measuring the optical density
+
# Preset temperature and wavelength for measuring the optical density
-
* Read and reference 1mL control solution (e.g. if E. coli was grown in LB/CAM) placed in disposable cuvette
+
# Read and reference 1mL control solution (e.g. if E. coli was grown in LB/CAM) placed in disposable cuvette
-
* Place 10µl cells into 1mL solution
+
# Place 10µl cells into 1mL solution
-
* Read the absorbance
+
# Read the absorbance
''Make note that when reading the optical density, must be referenced by a reference solution''
''Make note that when reading the optical density, must be referenced by a reference solution''
 +
 +
Return to [[Team:Columbia-Cooper-NYC/Protocols|Protocols Page]]

Revision as of 22:06, 16 August 2012

Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Safety Attributions Sponsor Us

Optical Density Measurement Protocol

  1. Preset temperature and wavelength for measuring the optical density
  2. Read and reference 1mL control solution (e.g. if E. coli was grown in LB/CAM) placed in disposable cuvette
  3. Place 10µl cells into 1mL solution
  4. Read the absorbance

Make note that when reading the optical density, must be referenced by a reference solution

Return to Protocols Page