Team:Columbia-Cooper-NYC/OD Measurement
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= Optical Density Measurement Protocol = | = Optical Density Measurement Protocol = | ||
- | + | # Preset temperature and wavelength for measuring the optical density | |
- | + | # Read and reference 1mL control solution (e.g. if E. coli was grown in LB/CAM) placed in disposable cuvette | |
- | + | # Place 10µl cells into 1mL solution | |
- | + | # Read the absorbance | |
''Make note that when reading the optical density, must be referenced by a reference solution'' | ''Make note that when reading the optical density, must be referenced by a reference solution'' | ||
+ | |||
+ | Return to [[Team:Columbia-Cooper-NYC/Protocols|Protocols Page]] |
Revision as of 22:06, 16 August 2012
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Optical Density Measurement Protocol
- Preset temperature and wavelength for measuring the optical density
- Read and reference 1mL control solution (e.g. if E. coli was grown in LB/CAM) placed in disposable cuvette
- Place 10µl cells into 1mL solution
- Read the absorbance
Make note that when reading the optical density, must be referenced by a reference solution
Return to Protocols Page