Team:Columbia-Cooper-NYC/OD Measurement

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Latest revision as of 03:38, 4 October 2012


Optical Density Measurement Protocol

  1. Preset temperature and wavelength for measuring the optical density
  2. Read and reference 1mL control solution (e.g. if E. coli was grown in LB/CAM) placed in disposable cuvette
  3. Place 10µl cells into 1mL solution
  4. Read the absorbance

Make note that when reading the optical density, must be referenced by a reference solution

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