http://2012.igem.org/wiki/index.php?title=Team:Columbia-Cooper-NYC/Miniprep&feed=atom&action=history
Team:Columbia-Cooper-NYC/Miniprep - Revision history
2024-03-29T01:49:04Z
Revision history for this page on the wiki
MediaWiki 1.16.0
http://2012.igem.org/wiki/index.php?title=Team:Columbia-Cooper-NYC/Miniprep&diff=267411&oldid=prev
Ymakita at 03:41, 4 October 2012
2012-10-04T03:41:10Z
<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 03:41, 4 October 2012</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 164:</td>
<td colspan="2" class="diff-lineno">Line 164:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div id="m7" onmouseover="mcancelclosetime()" onmouseout="mclosetime()"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div id="m7" onmouseover="mcancelclosetime()" onmouseout="mclosetime()"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <a href="https://2012.igem.org/Team:Columbia-Cooper-NYC/Safety">Safety</a></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <a href="https://2012.igem.org/Team:Columbia-Cooper-NYC/Safety">Safety</a></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <del class="diffchange diffchange-inline"><a href="https://2012.igem.org/Security">Security</a></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del></div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href="#" onmouseover="mopen('m8')" onmouseout="mclosetime()">Sponsors</a></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href="#" onmouseover="mopen('m8')" onmouseout="mclosetime()">Sponsors</a></div></td></tr>
</table>
Ymakita
http://2012.igem.org/wiki/index.php?title=Team:Columbia-Cooper-NYC/Miniprep&diff=254155&oldid=prev
Ymakita at 04:22, 3 October 2012
2012-10-03T04:22:20Z
<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 04:22, 3 October 2012</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 159:</td>
<td colspan="2" class="diff-lineno">Line 159:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div id="m6" onmouseover="mcancelclosetime()" onmouseout="mclosetime()"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div id="m6" onmouseover="mcancelclosetime()" onmouseout="mclosetime()"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <a href="https://2012.igem.org/Team:Columbia-Cooper-NYC/Outreach">Outreach</a></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <a href="https://2012.igem.org/Team:Columbia-Cooper-NYC/Outreach">Outreach</a></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <a href="https://2012.igem.org/Team:Columbia-Cooper-NYC/Collaborations">Collaboration</a></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </li></div></td></tr>
</table>
Ymakita
http://2012.igem.org/wiki/index.php?title=Team:Columbia-Cooper-NYC/Miniprep&diff=253268&oldid=prev
Ymakita at 02:25, 3 October 2012
2012-10-03T02:25:51Z
<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 02:25, 3 October 2012</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 198:</td>
<td colspan="2" class="diff-lineno">Line 198:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Discard the flow-through, and centrifuge for an additional 1-3 minutes to remove residual wash buffer</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Discard the flow-through, and centrifuge for an additional 1-3 minutes to remove residual wash buffer</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># To elute DNA, place the QIAprep column in a clean 1.5ml microcentrifuge tube. Add 50µl water to center of QIAprep spin column, let stand for 1 minute and centrifuge for 1-3 minutes.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># To elute DNA, place the QIAprep column in a clean 1.5ml microcentrifuge tube. Add 50µl water to center of QIAprep spin column, let stand for 1 minute and centrifuge for 1-3 minutes.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Return to [[Team:Columbia-Cooper-NYC/Protocols|Protocols Page]]</ins></div></td></tr>
</table>
Ymakita
http://2012.igem.org/wiki/index.php?title=Team:Columbia-Cooper-NYC/Miniprep&diff=253230&oldid=prev
Ymakita at 02:22, 3 October 2012
2012-10-03T02:22:05Z
<p></p>
<a href="http://2012.igem.org/wiki/index.php?title=Team:Columbia-Cooper-NYC/Miniprep&diff=253230&oldid=247514">Show changes</a>
Ymakita
http://2012.igem.org/wiki/index.php?title=Team:Columbia-Cooper-NYC/Miniprep&diff=247514&oldid=prev
Ymakita: /* Plasmid Isolation Protocol */
2012-10-01T23:53:22Z
<p><span class="autocomment">Plasmid Isolation Protocol</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 23:53, 1 October 2012</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 1:</td>
<td colspan="2" class="diff-lineno">Line 1:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>= Plasmid Isolation Protocol =</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>= Plasmid Isolation Protocol =</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Following is a plasmid isolation protocol provided by QIAGEN:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Following is a plasmid isolation protocol provided by QIAGEN:</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> ° µl</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Resuspend pelleted bacterial cells in 250µl Buffer P1 and transfer to a microcentrifuge tube</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Resuspend pelleted bacterial cells in 250µl Buffer P1 and transfer to a microcentrifuge tube</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Add 250µl Buffer P2 and mix thoroughly by inverting the tube 4-6 times</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Add 250µl Buffer P2 and mix thoroughly by inverting the tube 4-6 times</div></td></tr>
</table>
Ymakita
http://2012.igem.org/wiki/index.php?title=Team:Columbia-Cooper-NYC/Miniprep&diff=247512&oldid=prev
Ymakita: /* Plasmid Isolation Protocol */
2012-10-01T23:53:06Z
<p><span class="autocomment">Plasmid Isolation Protocol</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 23:53, 1 October 2012</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 1:</td>
<td colspan="2" class="diff-lineno">Line 1:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>= Plasmid Isolation Protocol =</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>= Plasmid Isolation Protocol =</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">* Following is a plasmid isolation protocol provided by QIAGEN:</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> ° µl</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"># Resuspend pelleted bacterial cells in 250µl Buffer P1 and transfer to a microcentrifuge tube</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"># Add 250µl Buffer P2 and mix thoroughly by inverting the tube 4-6 times</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"># Add 350µl Buffer N3 and mix immediately and thoroughly by inverting the tube 4-6 times</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"># Centrifuge for 10 min. at 13,000 rpm in a table-top microcentrifuge</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"># Apply the supernatant (from step 4) to the QIAprep spin column by decanting</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"># Centrifuge for 30-60 seconds and discard the flow-through</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"># Wash QIAspin column by adding 0.75ml Buffer PE and centrifuging for 30-60 seconds</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"># Discard the flow-through, and centrifuge for an additional 1-3 minutes to remove residual wash buffer</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"># To elute DNA, place the QIAprep column in a clean 1.5ml microcentrifuge tube. Add 50µl water to center of QIAprep spin column, let stand for 1 minute and centrifuge for 1-3 minutes.</ins></div></td></tr>
</table>
Ymakita
http://2012.igem.org/wiki/index.php?title=Team:Columbia-Cooper-NYC/Miniprep&diff=247462&oldid=prev
Ymakita: Created page with "= Plasmid Isolation Protocol ="
2012-10-01T23:38:42Z
<p>Created page with "= Plasmid Isolation Protocol ="</p>
<p><b>New page</b></p><div>= Plasmid Isolation Protocol =</div>
Ymakita