Team:Columbia-Cooper-NYC/Making Liquid Media
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+ | <i>Credit for protocol goes to Jason Candreva, Research Associate, Columbia University</i> |
Revision as of 03:14, 4 October 2012
Liquid Media Protocol
For 1 liter of liquid media:
- Combine in a large beaker, while stirring with a magnetic bar:
- 1 L of distilled water
- 0.8 g of ammonium sulfate (NH4)2SO4
- 1.0 g of magnesium sulfate MgSO4
- 0.4 g of dipotassium phosphate K2HPO4
- 5.0 mL o MD-TMS
- Using sulfuric acid (H2PO4), adjust pH of solution to 2.1. This should take about 0.5 mL of H2PO4
- Add 20 g of FeSO4.7H20
- Using sulfuric acid, adjust pH of solution to 1.8-1.85
- Once solution appears homogeneous, filter sterilize
Credit for protocol goes to Jason Candreva, Research Associate, Columbia University