Team:Columbia-Cooper-NYC/Making Liquid Media

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<LI>1.0 g of magnesium sulfate MgSO4
<LI>1.0 g of magnesium sulfate MgSO4
<LI>0.4 g of dipotassium phosphate K2HPO4
<LI>0.4 g of dipotassium phosphate K2HPO4
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<LI>5.0 mL o MD-TMS
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<LI>5.0 mL of MD-TMS
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<LI>Using sulfuric acid (H2PO4), adjust pH of solution to 2.1. This should take about 0.5 mL of H2PO4
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<LI>Using sulfuric acid (H2SO4), adjust pH of solution to 2.1. (This should take about 0.5 mL of H2SO4.)
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<LI>Add 20 g of FeSO4.7H20
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<LI>Add 20 g of FeSO4*7H20
<LI>Using sulfuric acid, adjust pH of solution to 1.8-1.85
<LI>Using sulfuric acid, adjust pH of solution to 1.8-1.85
<LI>Once solution appears homogeneous, filter sterilize
<LI>Once solution appears homogeneous, filter sterilize
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<i>Credit for protocol goes to Jason Candreva, Research Associate, Columbia University</i>
<i>Credit for protocol goes to Jason Candreva, Research Associate, Columbia University</i>
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Return to [[Team:Columbia-Cooper-NYC/Protocols|Protocols Page]]

Latest revision as of 07:47, 7 January 2013


Liquid Media Protocol

For 1 liter of liquid media:

  1. Combine in a large beaker, while stirring with a magnetic bar:
    • 1 L of distilled water
    • 0.8 g of ammonium sulfate (NH4)2SO4
    • 1.0 g of magnesium sulfate MgSO4
    • 0.4 g of dipotassium phosphate K2HPO4
    • 5.0 mL of MD-TMS
  2. Using sulfuric acid (H2SO4), adjust pH of solution to 2.1. (This should take about 0.5 mL of H2SO4.)
  3. Add 20 g of FeSO4*7H20
  4. Using sulfuric acid, adjust pH of solution to 1.8-1.85
  5. Once solution appears homogeneous, filter sterilize

Credit for protocol goes to Jason Candreva, Research Associate, Columbia University

Return to Protocols Page

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