Team:Columbia-Cooper-NYC/Main

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<div style="text-align: right;" class="top-links">[https://igem.org/Login Login] [https://igem.org/Logout Logout]
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[https://2012.igem.org/wiki/index.php?title=Talk:Team:Columbia-Cooper-NYC&action=edit discussion] 
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[https://2012.igem.org/wiki/index.php?title=Team:Columbia-Cooper-NYC/Main&action=edit edit]
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[https://2012.igem.org/wiki/index.php?title=Team:Columbia-Cooper-NYC/Main&action=history history]
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<h1 class="firstHeading">Team:Cambridge</h1>
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<a href="https://2012.igem.org/Team:Columbia-Cooper-NYC/Team">Biographies</a>
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<a href="https://2012.igem.org/Team:Columbia-Cooper-NYC/Facilitators">Facilitators</a>
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<a href="https://2012.igem.org/Team:Columbia-Cooper-NYC/Universities">The University(s)</a>
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<a href="https://igem.org/Team.cgi?year=2012">Official Profile</a>
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<a href="https://2012.igem.org/Team:Columbia-Cooper-NYC/Overview">Overview</a>
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                <a href="https://2012.igem.org/Team:Columbia-Cooper-NYC/Data_and_Conclusions">Data and Conclusions</a>
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                <a href="https://2012.igem.org/Team:Columbia-Cooper-NYC/Future_Directions">Future Directions</a>
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                <a href="https://2012.igem.org/Team:Columbia-Cooper-NYC/References">References</a>
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<a href="https://2012.igem.org/Team:Columbia-Cooper-NYC/Parts_Submitted">Parts Submitted</a>
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<a href="https://2012.igem.org/Team:Columbia-Cooper-NYC/Columbia_notebook_2">Genetics</a>
 +
<a href="https://2012.igem.org/Team:Columbia-Cooper-NYC/Columbia_notebook_1">Copper Etching</a>
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<a href="https://2012.igem.org/Team:Columbia-Cooper-NYC/Protocols">Protocols</a>
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        <li><a href="#" onmouseover="mopen('m6')" onmouseout="mclosetime()">Human Practice</a>
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<a href="https://2012.igem.org/Team:Columbia-Cooper-NYC/Outreach">Outreach</a>
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        <li><a href="https://2012.igem.org/Team:Columbia-Cooper-NYC/Safety" onmouseover="mopen('m7')" onmouseout="mclosetime()">Safety</a>
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                <a href="https://2012.igem.org/Team:Columbia-Cooper-NYC/Safety">Safety</a>
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</div>
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</li>
 +
        <li><a href="#" onmouseover="mopen('m8')" onmouseout="mclosetime()">Sponsors</a>
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                <a href="https://2012.igem.org/Team:Columbia-Cooper-NYC/Sponsor_Us">Sponsor Us</a>
 +
<a href="https://2012.igem.org/Team:Columbia-Cooper-NYC/Acknowledgements">Acknowledgements</a>
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                                            Columbia & Cooper 2012 Team</h2>
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                                            Columbia University</h2>
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                                            The Cooper Union for the Advancement of Science and Art</h2>
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                                            Genetics</h2>
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                                        <h2>
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                                            Copper Etching</h2>
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                                        <h2>
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                                            The Effect of A. ferrooxidans</h2>
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                                            Maker Faire (Copyright © Andrew Cribb)</h2>
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-->
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<div style="width:950px; background-color:#5C3317;" class="secheader">The Project</div>
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<p style="line-height:140%; padding-left:10px; padding-top:10px; padding-right:10px;text-align:justify; font-size:90%;">
 +
The Columbia-Cooper iGEM team is working with Acidithiobacillus ferrooxidans to create a light-controlled printed circuit board manufacturing process. This bacteria’s metabolism relies on its ability to oxidize iron; the iron can then be used to oxidize, and in turn solubilize, copper. By genetically altering the bacteria, we will install a light sensitive mechanism which will enable controlled copper etching, leaving a finished circuit board. Once a blank printed circuit board is placed in a thin layer of solid media, the bacteria will be applied onto the surface of the media and light of the appropriate wavelength will be focused on it in a desired pattern. The light sensitive mechanism in A. ferrooxidans will activate a self-destruct mechanism preventing copper etching in these locations. In the end, the circuit board will be "etched" by the bacteria everywhere that is not illuminated, leaving a desired pattern on the circuit board under the cells in the path of the light. Click <a style="color:#6bbe00 !important" href="https://2012.igem.org/Team:Columbia-Cooper-NYC/Overview">here</a> for details. </p>
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<div id="lefttop" style="background:#B87333; background-image:url('https://static.igem.org/mediawiki/2010/a/ad/Cam-Gradient-Overlay.png');" class="secheader">iGEM</div>
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 +
<p style="line-height:140%; padding-left:10px; padding-top:10px; padding-right:10px;text-align:justify; font-size:90%;"> The <a style="color:#6bbe00 !important" href="https://igem.org/Main_Page">International Genetically Engineered Machine competition (iGEM)</a> is the premiere undergraduate Synthetic Biology competition. Student teams are given a kit of biological parts from the <a style="color:#6bbe00 !important" href="http://partsregistry.org/Main_Page">Registry of Standard Biological Parts</a>. Working at their own schools over the summer, they use these and new parts of their own design to build biological systems and operate them in living cells. This project design and competition format is an exceptionally motivating and effective teaching method.</p>
 +
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<div id="ctop" style="background:#238E23;background-image:url('https://static.igem.org/mediawiki/2010/a/ad/Cam-Gradient-Overlay.png')" class="secheader">Genetics</div>
 +
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background: -moz-linear-gradient(top, #f2f2f2, #f8f8f8); color:#333333; height:350px;">
 +
<p style="line-height:140%; padding-left:10px; padding-top:10px; padding-right:10px;text-align:justify; font-size:90%;">Our Genetics Team was responsible for designing and implementing a series of genes which would allow us to exert light based spatial control of apoptosis in E.coli, and eventually transition into A. ferrooxidans. This design includes the expression of a light sensor, which, when exposed to specific wavelengths of light, triggers transcription of a lysis cassette.  A few of the parts which we experimented with are <a style="color:#6bbe00 !important" href="http://partsregistry.org/Part:BBa_K952000">BBa_K952000</a>, <a style="color:#6bbe00 !important" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K592016">BBa_K592016</a>, and <a style="color:#6bbe00 !important" href="http://partsregistry.org/Part:BBa_K124017">BBa_K124017</a>.
 +
 
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<div id="rtop" style="background:#B87333;background-image:url('https://static.igem.org/mediawiki/2010/a/ad/Cam-Gradient-Overlay.png')" class="secheader">Copper Etching</div>
 +
<div id="ccontent" style="background:-webkit-gradient(linear, left top, left bottom, from(#f2f2f2), to(#f8f8f8));
 +
background: -moz-linear-gradient(top, #f2f2f2, #f8f8f8); color:#333333; height:350px;">
 +
<p style="line-height:140%; padding-left:10px; padding-top:10px; padding-right:10px;text-align:justify; font-size:77%;"> Our project is to revamp a chemical and mechanical manufacturing process, so in addition to working with DNA, we also worked on macro-scale batches of copper foil, the bacteria A. ferrooxidans. Using our liquid media, we were able to grow ferrooxidans and simultaneously etch copper foil much faster than the basal rate (i.e. copper in the liquid media without ferrooxidans). Since our PCB manufacturing process requires the ferrooxidans to "stay put", we have been developing a solid agar-based media, on which the ferrooxidans grow, and below which the copper is etched, again faster than the basal rate.  Click <a style="color:#6bbe00 !important" href="https://2012.igem.org/Team:Columbia-Cooper-NYC/Columbia_notebook_1">here</a> for details</a>.
 +
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<div id="lefttop" style="background:#238E23; background-image:url('https://static.igem.org/mediawiki/2010/a/ad/Cam-Gradient-Overlay.png');" class="secheader">Biobrick</div>
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 +
<p style="line-height:140%; padding-left:10px; padding-top:10px; padding-right:10px;text-align:justify; font-size:90%;"> Our team submitted multiple composite parts, which were built from preexisting Biobricks and used in our project. We also submitted a novel red light sensing protein domain, FphA753s, which was constructed to conform to the RFC25 – Freiburg fusion standard, and is intended for future use to allow light based control of transcription. Click
 +
 +
<a style="color:#6bbe00 !important" href="https://2012.igem.org/Team:Columbia-Cooper-NYC/Parts_Submitted">here</a> for a full list of our submitted parts.</p>
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<p style="line-height:140%; padding-left:10px; padding-top:10px; padding-right:10px;text-align:justify; font-size:90%;"> Our team organized a booth at the 2012 Maker Faire in New York City to share the work we've been doing with the public. We also made 3 interactive games to teach children about recombinant DNA and the various forms of bacterial transformation. Click <a style="color:#6bbe00 !important" href="https://2012.igem.org/Team:Columbia-Cooper-NYC/Outreach">here</a> for more info.</a>
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Columbia<br />University
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Cooper<br />Union
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Latest revision as of 21:13, 22 October 2012

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Team:Cambridge

From 2010.igem.org


  • Columbia & Cooper 2012 Team

  • Columbia University

  • The Cooper Union for the Advancement of Science and Art

  • Genetics

  • Copper Etching

  • The Effect of A. ferrooxidans

  • Maker Faire (Copyright © Andrew Cribb)

Retrieved from "http://2012.igem.org/Team:Columbia-Cooper-NYC/Main"