Team:Columbia-Cooper-NYC/Electroporation

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Electroporation Protocol

  • Place cuvettes and competent cells into ice
  • Add 1µl of DNA to 50µl of competent cells into 1mL cuvettes as quickly as possible
  • Keep cuvettes in ice until electroporation
  • Electroporate cells at 1800V
  • Place bacteria back into tube and add 100-200µl of LB
  • Place samples in shaker for 37C for 1 hour
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