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Team:Columbia-Cooper-NYC/Electroporation
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(Created page with "= Electroporation Protocol = * Place cuvettes and competent cells into ice * Add 1µl of DNA to 50µl of competent cells into 1mL cuvettes as quickly as possible * Keep cuvettes ...")
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(Created page with "= Electroporation Protocol = * Place cuvettes and competent cells into ice * Add 1µl of DNA to 50µl of competent cells into 1mL cuvettes as quickly as possible * Keep cuvettes ...")
Newer edit →
Revision as of 01:53, 3 October 2012
Electroporation Protocol
- Place cuvettes and competent cells into ice
- Add 1µl of DNA to 50µl of competent cells into 1mL cuvettes as quickly as possible
- Keep cuvettes in ice until electroporation
- Electroporate cells at 1800V
- Place bacteria back into tube and add 100-200µl of LB
- Place samples in shaker for 37C for 1 hour
