Team:Columbia-Cooper-NYC/Electroporation

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= Electroporation Protocol =
= Electroporation Protocol =
-
* Place cuvettes and competent cells into ice
+
* The following is the electroporation protocol provided by bioline:
-
* Add 1µl of DNA to 50µl of competent cells into 1mL cuvettes as quickly as possible
+
# Place cuvettes and competent cells into ice
-
* Keep cuvettes in ice until electroporation
+
# Add 1µl of DNA to 50µl of competent cells into 1mL cuvettes as quickly as possible
-
* Electroporate cells at 1800V
+
# Keep cuvettes in ice until electroporation
-
* Place bacteria back into tube and add 100-200µl of LB
+
# Electroporate cells at 1800V
-
* Place samples in shaker for 37C for 1 hour
+
# Place bacteria back into tube and add 100-200µl of LB
 +
# Place samples in shaker for 37C for 1 hour
 +
 
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Return to [[Team:Columbia-Cooper-NYC/Protocols|Protocols Page]]

Latest revision as of 03:41, 4 October 2012


Electroporation Protocol

  • The following is the electroporation protocol provided by bioline:
  1. Place cuvettes and competent cells into ice
  2. Add 1µl of DNA to 50µl of competent cells into 1mL cuvettes as quickly as possible
  3. Keep cuvettes in ice until electroporation
  4. Electroporate cells at 1800V
  5. Place bacteria back into tube and add 100-200µl of LB
  6. Place samples in shaker for 37C for 1 hour

Return to Protocols Page