Team:Columbia-Cooper-NYC/Columbia notebook 2

From 2012.igem.org

(Difference between revisions)
(July, 2012)
(July, 2012)
Line 40: Line 40:
*# 2 samples: kill gene
*# 2 samples: kill gene
*# 2 samples: pET20b vector
*# 2 samples: pET20b vector
 +
=== Week 3 ===
 +
==== Monday, 16th ====
 +
==== Tuesday, 17th ====
 +
==== Wednesday, 18th ====
 +
==== Thursday, 19th ====
 +
=== Week 4 ===
 +
==== Tuesday, 24th ====
 +
==== Thursday, 26th ====
 +
==== Friday, 27th ====
 +
==== Saturday, 28th ====
 +
=== Week 5 ===

Revision as of 02:47, 30 July 2012

Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Safety Attributions Sponsor Us

Contents

Columbia Genetics Lab Notebook

July, 2012

Week 1

Thursday, 5th

  • Re-hydrated plasmids with 50µl of LB and Kanamycin solution
  • Stored solution at 37°C incubator overnight

Friday, 6th

  • Purified pET26b vector using standard DNA purification protocol

Week 2

Monday, 9th

  • Received kill gene Bba-K124017 from plate 3, 20M
  • Re-hydrated DNA according to standard iGEM re-hydration protocol

Tuesday, 10th

  • Contacted professors at Germany in hopes to receive copies of fungal phytochrome FphA

Wednesday, 11th

  • Received confirmation by professors at Germany for FphA to be sent to Columbia University
  • Conducted transformation using electroporation with competent bacteria (marked by resistance to Kanamycin)
    1. Control: 1µl of deionized water with abt. and 60µl of bacteria cells
    2. Variable: 1µl of re-hydrated kill gene and 60µl of bacteria cells
  • Placed both samples after electroporation into 200µl of preprepared LB
  • Placed samples in shaker 37°C for 30 minutes

Thursday, 12th

  • Grew 1 colony of transformed bacteria in 5mL of Kanamycin and LB solution
    • Note: Using pET20b vector over pET26b vector from glycerol stock solution

Friday, 13th

  • Isolated 4 samples of plasmid using standard plasmid isolation protocol
    1. 2 samples: kill gene
    2. 2 samples: pET20b vector

Week 3

Monday, 16th

Tuesday, 17th

Wednesday, 18th

Thursday, 19th

Week 4

Tuesday, 24th

Thursday, 26th

Friday, 27th

Saturday, 28th

Week 5