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| - | * @name jquery-lightbox-0.5.css
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| - | * @category jQuery plugin
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| - | * @example Visit http://leandrovieira.com/projects/jquery/lightbox/ for more informations about this jQuery plugin
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| - | <div id="lefttop" style="background:#96d446; background-image:url('https://static.igem.org/mediawiki/2010/a/ad/Cam-Gradient-Overlay.png');" class="secheader">Abstract</div>
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| - | <div id="leftcontent" style="background:-webkit-gradient(linear, left top, left bottom, from(#f2f2f2), to(#f8f8f8));
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| - | background: -moz-linear-gradient(top, #f2f2f2, #f8f8f8); color:#333333; height:150px;">
| |
| - | <p style="line-height:140%; padding-left:10px; padding-top:10px; padding-right:10px;text-align:justify; font-size:90%;"> We placed genes from <a style="color:#6bbe00 !important" href="https://2010.igem.org/Team:Cambridge/Bioluminescence">fireflies</a> and bioluminescent <a style="color:#6bbe00 !important" href="https://2010.igem.org/Team:Cambridge/Bioluminescence/Bacterial_Luciferases">bacteria</a> into E.coli. Codon optimisation and single amino acid mutagenesis allowed us to generate bright light output in a range of different colours. Future applications include and quantitative <a style="color:#6bbe00 !important" href="https://2010.igem.org/Team:Cambridge/Tools/microMeasure">biosensors</a> and biological <a style="color:#6bbe00 !important" href="https://2010.igem.org/Team:Cambridge/Tools/Lighting">alternatives</a> to conventional lighting.</p>
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| - | <div id="ctop" style="background:#fad72a;background-image:url('https://static.igem.org/mediawiki/2010/a/ad/Cam-Gradient-Overlay.png')" class="secheader">Multimedia</div>
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| - | <div id="ccontent" style="background:-webkit-gradient(linear, left top, left bottom, from(#f2f2f2), to(#f8f8f8));
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| - | background: -moz-linear-gradient(top, #f2f2f2, #f8f8f8); color:#333333; height:150px;">
| |
| - | <p style="line-height:140%; padding-left:10px; padding-top:10px; padding-right:10px;text-align:justify; font-size:90%;">If you want a break from hard-core science, check out our Gibson Assembly <a style="color:#d9b400 !important" onclick="displayGibson('http://www.youtube.com/v/WCWjJFU1be8?fs=1&hl=en_GB'); return false;" href="http://www.youtube.com/watch?v=WCWjJFU1be8">music video</a>.<br /><br />You can also see view videos of our <a style="color:#d9b400 !important" onclick="displayGibson('http://www.youtube.com/v/tUFscEVK5Ks?fs=1&hl=en_GB'); return false;" href="http://www.youtube.com/watch?v=WCWjJFU1be8">bacterial bubble lamp</a> and <a style="color:#d9b400 !important" onclick="displayGibson('http://www.cambridgeigem.org/video.php'); return false;" href="http://www.cambridgeigem.org/video.php?nojs=true">project overview</a>.
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| - | newDiv.setAttribute('id',"GibsonVidOverlay");
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| - | $('body')[0].removeChild($('#GibsonVidOverlay')[0]);
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| - | <div id="rtop" style="background:#fb5c2b;background-image:url('https://static.igem.org/mediawiki/2010/a/ad/Cam-Gradient-Overlay.png')" class="secheader">Tools</div>
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| - | <div id="rcontent" style="background:-webkit-gradient(linear, left top, left bottom, from(#f2f2f2), to(#f8f8f8));
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| - | background: -moz-linear-gradient(top, #f2f2f2, #f8f8f8); color:#333333; height:150px; margin-top:5px; position:relative;">
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| - | <div style="position:relative; top:10px;">
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| - | <a href="http://www.gibthon.org" id="leftbox" style="background:url('https://static.igem.org/mediawiki/2010/5/53/Cambridge-Gibthon.png'); position:absolute; width:120px; height:100px; left:0;top:0; background-repeat:no-repeat; display:block;text-align:center; padding-top:70px; background-position: center top; color:#e53500 !important;" >
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| - | Gibson<br />Assembly
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| - | </a>
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| - | <a href="http://www.gibthon.org/ligate.html" id="rightbox" style="background:url('https://static.igem.org/mediawiki/2010/b/b1/Cambridge-Flask.png'); position:absolute; width:120px; height:100px; left:120px; text-align:center;top:0; background-repeat:no-repeat;display:block; padding-top:70px; background-position: center top; color:#e53500 !important;" />
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| - | Ligation<br />Calculator
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| - | </a>
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| - | <div style="width:730px; background-color:#386abc;" class="secheader">The Project</div>
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| - | <div class="contwrap">
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| - | <img src="https://static.igem.org/mediawiki/2010/7/70/Front.jpg" style="margin-left:4px; margin-bottom:5px; width:710px; margin-top:-2px;">
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| - | <div style="height:30px; position:relative; font-size:12px; color:#6e6e6e; line-height:15px;">
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| - | <div style="position:absolute; width:230px; left:5px; text-align:center;">A flask lit by E. coli transformed with one of our constructs </div>
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| - | <div style="position:absolute; width:230px; left:240px; text-align:center;">E. coli transformed with our different coloured bioluminescent systems</div>
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| - | <div style="position:absolute; width:220px; left:485px; text-align:center;">Team members illuminated only by our bacteria</div>
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| - | </div>
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The Columbia-Cooper iGEM team is working with Acidithiobacillus ferrooxidans to create a light-controlled printed circuit board manufacturing process. This bacteria’s metabolism relies on its ability to oxidize iron; the iron can then be used to oxidize, and in turn solubilize, copper. By genetically altering the bacteria, we intend to install a light sensitive mechanism which will enable us to etch copper in a desired pattern, leaving a finished circuit board. Once a blank printed circuit board is placed in a thin layer of solid media, the bacteria will be applied onto the surface of the media and light will be focused on it in a desired pattern. The light sensitive mechanism in ferrooxidans would activate and self-destruct in the pathway of the light. In the end, the circuit board will be "etched" by the bacteria everywhere but the illuminated spots, leaving the desired pattern behind on the circuit board.
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