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Team:Colorado State/Project
From 2012.igem.org
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n-terminus | n-terminus | ||
c-terminus | c-terminus | ||
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-Prolyl Endoprotease-A.niger | -Prolyl Endoprotease-A.niger | ||
viable option? | viable option? | ||
Revision as of 16:09, 19 June 2012
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Overall project
We are currently pursuing the concept of brewing a gluten-free beer using a yeast that secretes an enzyme to break down the gluten. (1-2 paragraphs)
Project Details
-yeast
assemble plasmids His tag plasmids n-terminus c-terminus clone mating factor-alpha
-bacteria
assemble plasmids His tag plasmids n-terminus c-terminus secretion
-Prolyl Endoprotease-A.niger
viable option? source? cloning vs synthesize E.coli yeast
-functional protease assay
fluorophore/quencher mass spec elisa
