Team:Colorado State

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 +
</script>
 +
<!--Script to activate dropdown menu function. Uses jQuery syntax.-->
 +
<script type="text/javascript">
 +
$(document).ready(function () {
 +
$(‘.dropdown’).each(function () {
 +
$(this).parent().eq(0).hover(function () {
 +
$(‘.dropdown:eq(0)’, this).show();
 +
}, function () {
 +
$(‘.dropdown:eq(0)’, this).hide();
 +
});
 +
});
 +
});
 +
 +
</script>
 +
<!--Circle effects. jQuery custom functions make all of us happy. ^_^ -->
 +
<script type="text/javascript">
 +
$(document).ready(function(){
 +
 +
jQuery.fn.setcircle = function(){
 +
this.css({
 +
"display":"block",
 +
"position":"absolute",
 +
"margin-left":790,
 +
"margin-top":-250,
 +
});
 +
}
 +
 +
jQuery.fn.resetcircle = function(){
 +
this.hide();
 +
$('#home #circle0').show();
 +
$('#team #circle1').show();
 +
$('#project #circle2').show();
 +
$('#parts #circle3').show();
 +
$('#notebook #circle4').show();
 +
$('#outreach #circle5').show();
 +
$('#sponsors #circle6').show();
 +
}
 +
//homebutton set
 +
$('#homebutton').mouseover(function(){
 +
$('.headercircle').hide();
 +
$('#circle0').show();
 +
$('#circle0').setcircle();
 +
});
 +
$('#homebutton').mouseout(function(){
 +
$('#circle0').resetcircle();
 +
});
 +
 +
//teambutton set
 +
$('#teambutton').mouseover(function(){
 +
$('.headercircle').hide();
 +
$('#circle1').show();
 +
$('#circle1').setcircle();
 +
});
 +
$('#teambutton').mouseout(function(){
 +
$('#circle1').resetcircle();
 +
});
 +
 +
//projectbutton set
 +
$('#projectbutton').mouseover(function(){
 +
$('.headercircle').hide();
 +
$('#circle2').show();
 +
$('#circle2').setcircle();
 +
});
 +
$('#projectbutton').mouseout(function(){
 +
$('#circle2').resetcircle();
 +
});
 +
 +
//partsbutton set
 +
$('#partsbutton').mouseover(function(){
 +
$('.headercircle').hide();
 +
$('#circle3').show();
 +
$('#circle3').setcircle();
 +
});
 +
$('#partsbutton').mouseout(function(){
 +
$('#circle3').resetcircle();
 +
});
 +
 +
//notebookbutton set
 +
$('#notebookbutton').mouseover(function(){
 +
$('.headercircle').hide();
 +
$('#circle4').show();
 +
$('#circle4').setcircle();
 +
});
 +
$('#notebookbutton').mouseout(function(){
 +
$('#circle4').resetcircle();
 +
});
 +
 +
//outreachbutton set
 +
$('#outreachbutton').mouseover(function(){
 +
$('.headercircle').hide();
 +
$('#circle5').show();
 +
$('#circle5').setcircle();
 +
});
 +
$('#outreachbutton').mouseout(function(){
 +
$('#circle5').resetcircle();
 +
});
 +
 +
//sponsorsbutton set
 +
$('#sponsorsbutton').mouseover(function(){
 +
$('.headercircle').hide();
 +
$('#circle6').show();
 +
$('#circle6').setcircle();
 +
});
 +
$('#sponsorsbutton').mouseout(function(){
 +
$('#circle6').resetcircle();
 +
});
 +
 +
//igembutton set
 +
$('#igembutton').mouseover(function(){
 +
$('.headercircle').hide();
 +
$('#circle7').show();
 +
$('#circle7').setcircle();
 +
});
 +
$('#igembutton').mouseout(function(){
 +
$('#circle7').resetcircle();
 +
});
 +
});
 +
</script>
 +
 +
 +
 +
<style>
 +
/***Master styling of text components, because we like consistency***/
 +
 +
h1, h2, h3, h4, h5, p, a{
 +
text-decoration: none;
 +
border: 0;
 +
font-family: Arial;
 +
}
 +
 +
a{
 +
color: #0063bd;
 +
}
 +
 +
#sidetext a, #sidetext p, #sidetext li a{
 +
color: white;
 +
font-family: Arial;
 +
}
 +
 +
a, a:visited{
 +
color: #0063bd;
 +
}
 +
 +
table tr{
 +
margin-top: 5px;
 +
margin-bottom: 5px;
 +
}
 +
 +
table td{
 +
margin-left: 5px;
 +
margin-right: 5px;
 +
}
 +
 +
#sponsors table tr td img{
 +
width: 200px;
 +
padding-right: 30px;
 +
padding-left: 10px;
 +
}
 +
 +
/***End master styling***/
 +
 +
 +
/***
 +
Minimal header: removes the search bar and header image and readjusts font colours in the menus.
 +
 +
Thanks a lot to the 2011 Brown-Stanford iGEM team for snippets of their code!
 +
Check out their wiki at https://2011.igem.org/Team:Brown-Stanford
 +
***/
 +
 +
div#top-section{ /*the div containing the entire top bar*/
 +
height: 20px;
 +
margin-left: auto;
 +
margin-right: auto;
 +
margin-bottom: 0px !important;
 +
margin-top: -14px;
 +
padding:0px 0px 0px 0px;;
 +
border: none;
 +
width: 100%;
 +
background: none;
 +
color: black;
 +
position:absolute;
 +
}
 +
 +
#p-logo { /*the header image*/
 +
height:0px;
 +
overflow:hidden;
 +
border:none;
 +
}
 +
 +
#content{
 +
margin-top: 14px;
 +
}
 +
 +
#search-controls {
 +
overflow:hidden;
 +
display:none;
 +
background: none;
 +
position: absolute;
 +
top: 170px;
 +
right: 40px;
 +
}
 +
 +
 +
div#header {
 +
width: 975px;
 +
text-align: left;
 +
margin-left: auto;
 +
margin-right: auto;
 +
margin-bottom: 0px !important;
 +
 +
 +
#menubar {
 +
position: absolute;
 +
background: none;
 +
color: black;
 +
}
 +
 +
.left-menu, .right-menu{
 +
position: absolute;
 +
background: none;
 +
color: white;
 +
}
 +
 +
.left-menu li, .right-menu li a {
 +
color: transparent !important;
 +
}
 +
 +
 +
.left-menu ul li, .right-menu ul li a{
 +
background: none;
 +
color: white;
 +
}
 +
 +
.right-menu li a:hover, .right-menu li a:visited, .right-menu li a:active {
 +
    color: white !important;
 +
}
 +
 +
/***End minimal header***/
 +
 +
 +
/***Now for our own stuff: personalized header images and navbar***/
 +
 +
#headercontainer{
 +
margin-left: 30px;
 +
}
 +
 +
#headerbar{
 +
width: 842px;
 +
height: 48px;
 +
background-image: url(https://static.igem.org/mediawiki/2011/e/ef/UCalgary_HeaderBarNew.png);
 +
position: absolute;
 +
margin-top: -25px;
 +
z-index: 99;
 +
}
 +
 +
/*div containing menu bar*/
 +
ul#topnav{
 +
display: table;
 +
margin: 0;
 +
padding: 0;
 +
left: 15px;
 +
top: 15px;
 +
list-style: none;
 +
position: relative;
 +
z-index: 100;
 +
}
 +
 +
ul#topnav a.navtext{
 +
position: relative;
 +
display: block;
 +
background-position: left top;
 +
margin-right: 12px;
 +
margin-left: 12px;
 +
}
 +
 +
ul#topnav li{
 +
margin: 0;
 +
padding: 0;
 +
list-style: none;
 +
display: table-cell;
 +
float: left;
 +
position: relative;
 +
}
 +
 +
ul#topnav li a{
 +
padding: 0;
 +
display: block;
 +
color: #f0f0f0;
 +
text-decoration: none;
 +
}
 +
 +
ul#topnav li:hover, ul#topnav a:hover{
 +
background-position: left bottom;
 +
}
 +
 +
/*Subnavigation*/
 +
ul.dropdown{
 +
margin: 0;
 +
padding: 0;
 +
padding-bottom: 10px;
 +
padding-top: 7px;
 +
display: block;
 +
position: absolute;
 +
z-index: 999;
 +
width: 200px;
 +
display: none;
 +
left: 0;
 +
/*Background of subnav*/
 +
background-color: #00365E;
 +
opacity: 0.77;
 +
}
 +
 +
ul.dropdown li{
 +
margin: 0;
 +
padding: 0;
 +
width: 200px;
 +
float: none;
 +
position: relative;
 +
list-style: none;
 +
display: block;
 +
}
 +
 +
ul.dropdown li a{
 +
display: block;
 +
}
 +
 +
 +
/*Establish sprites for each individual button*/
 +
#homebutton a{
 +
background-image: url(https://static.igem.org/mediawiki/2011/1/1d/UCalgary_MenuHome.png);
 +
width: 51px;
 +
height: 16px;
 +
}
 +
 +
#teambutton a{
 +
background-image: url(https://static.igem.org/mediawiki/2011/4/4f/UCalgary_MenuTeam.png);
 +
width: 48px;
 +
height: 16px;
 +
margin-bottom: 17px;
 +
}
 +
 +
#projectbutton a{
 +
background-image: url(https://static.igem.org/mediawiki/2011/f/f0/UCalgary_MenuProject.png);
 +
width: 62px;
 +
height: 19px;
 +
margin-bottom: 14px;
 +
}
 +
 +
#partsbutton a{
 +
background-image: url(https://static.igem.org/mediawiki/2011/e/e4/UCalgary_MenuParts.png);
 +
width: 45px;
 +
height: 16px;
 +
margin-bottom: 17px;
 +
}
 +
 +
#notebookbutton a{
 +
background-image: url(https://static.igem.org/mediawiki/2011/8/84/UCalgary_MenuNotebook.png);
 +
width: 84px;
 +
height: 16px;
 +
margin-bottom: 17px;
 +
}
 +
 +
#outreachbutton a{
 +
background-image: url(https://static.igem.org/mediawiki/2011/9/96/UCalgary_MenuOutreach.png);
 +
width: 80px;
 +
height: 17px;
 +
margin-bottom: 16px;
 +
}
 +
 +
#sponsorsbutton a{
 +
background-image: url(https://static.igem.org/mediawiki/2011/5/51/UCalgary_MenuSponsors.png);
 +
width: 84px;
 +
height: 20px;
 +
margin-bottom: 13px;
 +
}
 +
 +
#igembutton a{
 +
background-image: url(https://static.igem.org/mediawiki/2011/4/49/UCalgary_MenuiGEM.png);
 +
width: 46px;
 +
height: 17px;
 +
margin-bottom: 16px;
 +
}
 +
 +
.navtext{
 +
text-indent: -99999px; /*Shoots all HTML text buttons off the page*/
 +
}
 +
 +
/*Adjusting the subnavigation menu*/
 +
ul#topnav li#teambutton ul li a,
 +
ul#topnav li#projectbutton ul li a,
 +
ul#topnav li#partsbutton ul li a,
 +
ul#topnav li#notebookbutton ul li a,
 +
ul#topnav li#outreachbutton ul li a,
 +
ul#topnav li#sponsorsbutton ul li a{
 +
height: 35px;
 +
background-image: none;
 +
font-family: Arial;
 +
font-size: 17px;
 +
font-weight: 400;
 +
}
 +
 +
ul#topnav li ul li a{
 +
display: inline; /*correction of previous link style*/
 +
background-image: url(https://static.igem.org/mediawiki/2011/6/69/UCalgary_SubNavRepeater.png);
 +
background-repeat: repeat-x;
 +
padding-left: 12px;
 +
padding-right: 12px;
 +
}
 +
 +
/*Rollover settings*/
 +
ul#topnav li:hover ul{
 +
display: block;
 +
z-index: 999;
 +
}
 +
 +
ul#topnav li ul li a:hover{
 +
color: #49f0ff;
 +
}
 +
 +
/*Active state settings*/
 +
#home li#homebutton a,
 +
#team li#teambutton a,
 +
#project li#projectbutton a,
 +
#parts li#partsbutton a,
 +
#notebook li#notebookbutton a,
 +
#outreach li#outreachbutton a,
 +
#sponsors li#sponsorsbutton a{
 +
background-position: left bottom;
 +
}
 +
 +
/*Hide all circles*/
 +
img.headercircle{
 +
display: none;
 +
z-index: 250;
 +
}
 +
 +
/*Active circle showing*/
 +
#home #circle0,
 +
#team #circle1,
 +
#project #circle2,
 +
#parts #circle3,
 +
#notebook #circle4,
 +
#outreach #circle5,
 +
#sponsors #circle6{
 +
display: block;
 +
position: absolute;
 +
margin-left: 790px;
 +
margin-top: -250px;
 +
}
 +
 +
/***End menu stuff. Onto the rest of the header!***/
 +
 +
.firstHeading{
 +
text-indent: -99999px;
 +
}
 +
 +
#headbanner, #headbanner img{
 +
width: 906px;
 +
height: 216px;
 +
z-index: 0;
 +
}
 +
 +
#fb-button{
 +
position: absolute;
 +
margin-left: 872px;
 +
margin-top: -170px;
 +
}
 +
 +
#twitter-button{
 +
position: absolute;
 +
margin-left: 898px;
 +
margin-top: -170px;
 +
}
 +
 +
 +
/*...Not much, was there.*/
 +
 +
 +
</style>
 +
 +
 +
 +
 +
 +
</p><div id="headercontainer">
 +
<div id="headerbar">
 +
<ul id="topnav">
 +
<li id="homebutton"><a class="navtext" href="https://2011.igem.org/Team:Calgary">Home</a></li>
 +
<li id="teambutton">
 +
<a class="navtext" href="https://2011.igem.org/Team:Calgary/Team">Team</a>
 +
<ul class="dropdown">
 +
<li><a href="https://2011.igem.org/Team:Calgary/Team/Biographies">Biographies</a></li>
 +
<li><a href="https://2011.igem.org/Team:Calgary/Team/Facilitators">Facilitators</a></li>
 +
<li><a href="https://2011.igem.org/Team:Calgary/Team/About_The_University">The University</a></li>
 +
<li><a href="http://ung.igem.org/Team.cgi?id=640">Official Profile</a></li>
 +
</ul>
 +
</li>
 +
<li id="projectbutton">
 +
<a class="navtext" href="https://2011.igem.org/Team:Calgary/Project">Project</a>
 +
<ul class="dropdown">
 +
<li><a href="https://2011.igem.org/Team:Calgary/Project">Overview</a></li>
 +
<li><a href="https://2011.igem.org/Team:Calgary/Project/Promoter">Promoter</a></li>
 +
<li><a href="https://2011.igem.org/Team:Calgary/Project/Reporter">Reporter</a></li>
 +
<li><a href="https://2011.igem.org/Team:Calgary/Project/Chassis">Chassis</a></li>
 +
<li><a href="https://2011.igem.org/Team:Calgary/Project/Reporter/Prototype">Prototype</a></li>
 +
                        <li><a href="https://2011.igem.org/Team:Calgary/Project/DataPage">Data Page</a></li>
 +
                        <li><a href="https://2011.igem.org/Team:Calgary/Project/Acomplishments">Accomplishments</a></li>
 +
<li><a href="https://2011.igem.org/Team:Calgary/Project/Future_Directions">Future Directions</a></li>
 +
<li><a href="https://2011.igem.org/Team:Calgary/Project/References">References</a></li>
 +
</ul>
 +
</li>
 +
<li id="partsbutton">
 +
<a class="navtext" href="https://2011.igem.org/Team:Calgary/Parts">Parts</a>
 +
<ul class="dropdown">
 +
<li><a href="https://2011.igem.org/Team:Calgary/Parts">Parts Submitted</a></li>
 +
                        <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Attributions">Attributions</a></li>
 +
</ul>
 +
</li>
 +
<li id="notebookbutton">
 +
<a class="navtext" href="https://2011.igem.org/Team:Calgary/Notebook">Notebook</a>
 +
<ul class="dropdown">
 +
                        <li><a href="https://2011.igem.org/Team:Calgary/Notebook/PostRegionals">Post-Regionals</a></li>
 +
<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Calendar">Journal</a></li>
 +
<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols">Protocols</a></li>
 +
<li><a href="https://2011.igem.org/Team:Calgary/Safety">Safety</a></li>
 +
</ul>
 +
</li>
 +
<li id="outreachbutton">
 +
<a class="navtext" href="https://2011.igem.org/Team:Calgary/Outreach">Outreach</a>
 +
<ul class="dropdown">
 +
<li><a href="https://2011.igem.org/Team:Calgary/Outreach/Human">Human Practices</a></li>
 +
<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Conferences">Conferences</a></li>
 +
<li><a href="https://2011.igem.org/Team:Calgary/Outreach/Follow">Follow Us!</a></li>
 +
</ul>
 +
</li>
 +
<li id="sponsorsbutton">
 +
<a class="navtext" href="https://2011.igem.org/Team:Calgary/Sponsors">Sponsors</a>
 +
<ul class="dropdown">
 +
<li><a href="https://2011.igem.org/Team:Calgary/Sponsors">Sponsors</a></li>
 +
<li><a href="https://2011.igem.org/Team:Calgary/Sponsors/Acknowledgements">Acknowledgements</a></li>
 +
</ul>
 +
</li>
 +
<li id="igembutton">
 +
<a class="navtext" href="https://2011.igem.org" target="_blank">iGEM</a>
 +
</li>
 +
 +
</ul>
 +
</div>
 +
 +
<div style="position: relative;" id="headbanner">
 +
<img style="position: absolute; top: 0px; left: 0px; display: none; z-index: 5; opacity: 0; width: 906px; height: 216px;" src="https://static.igem.org/mediawiki/2011/1/1c/UCalgary_HeaderImage2.png">
 +
<img style="position: absolute; top: 0px; left: 0px; display: block; z-index: 6; opacity: 1; width: 906px; height: 216px;" src="https://static.igem.org/mediawiki/2011/0/04/UCalgary2011_MainPageHeaderImage1.png">
 +
<img style="position: absolute; top: 0px; left: 0px; display: none; z-index: 5; opacity: 0; width: 906px; height: 216px;" src="https://static.igem.org/mediawiki/2011/9/93/UCalgary2011_HeaderImage3.png">
 +
<img style="position: absolute; top: 0px; left: 0px; display: none; z-index: 5; opacity: 0; width: 906px; height: 216px;" src="https://static.igem.org/mediawiki/2011/d/d5/UCalgary2011_HeaderImage4.png">
 +
<img style="position: absolute; top: 0px; left: 0px; display: none; z-index: 5; opacity: 0; width: 906px; height: 216px;" src="https://static.igem.org/mediawiki/2011/f/f6/UCalgary2011_HeaderImage5.png">
 +
</div>
 +
 +
<!--Header Image Shuffling-->
 +
<script type="text/javascript">
 +
$(document).ready(function(){
 +
$('#headbanner').cycle({
 +
timeout: 8000
 +
});
 +
});
 +
</script>
 +
<!--Circles-->
 +
<img style="display: block; position: absolute; margin-left: 790px; margin-top: -250px;" class="headercircle" id="circle0" src="https://static.igem.org/mediawiki/2011/c/c1/UCalgary_CircleHome.png">
 +
<img style="display: none; position: absolute; margin-left: 790px; margin-top: -250px;" class="headercircle" id="circle1" src="https://static.igem.org/mediawiki/2011/d/d4/UCalgary_CircleTeam.png">
 +
<img style="display: none;" class="headercircle" id="circle2" src="https://static.igem.org/mediawiki/2011/7/7d/UCalgary_CircleProject.png">
 +
<img style="display: none;" class="headercircle" id="circle3" src="https://static.igem.org/mediawiki/2011/8/8d/UCalgary_CircleParts.png">
 +
<img style="display: none; position: absolute; margin-left: 790px; margin-top: -250px;" class="headercircle" id="circle4" src="https://static.igem.org/mediawiki/2011/0/03/UCalgary_CircleNotebook.png">
 +
<img style="display: none;" class="headercircle" id="circle5" src="https://static.igem.org/mediawiki/2011/8/84/UCalgary_CircleOutreach.png">
 +
<img style="display: none;" class="headercircle" id="circle6" src="https://static.igem.org/mediawiki/2011/3/3c/UCalgary_CircleSponsors.png">
 +
<img style="display: none;" class="headercircle" id="circle7" src="https://static.igem.org/mediawiki/2011/8/85/UCalgary_CircleiGEM.png">
 +
</div>
 +
 +
 +
 +
 +
<p></p><p>
 +
 +
 +
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<a style="position: absolute; float: right; margin-left: 788px; margin-top: 11px;" href="https://2011.igem.org/Team:Calgary/Notebook/PostRegionals"><img style="width: 150px;" src="https://static.igem.org/mediawiki/2011/0/0d/UCalgary2011_Fred_Flag.png"></a>
 +
<a style="position: relative; float: right; left: -46px; margin-top: 75px;" href="https://2011.igem.org/Team:Calgary/Project/DataPage"><img style="width: 150px;" src="https://static.igem.org/mediawiki/2011/7/7f/UCalgary2011_Fred_Data_Page.png"></a>
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<h1 id="pagetitle">Welcome!</h1>
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<img class="boxtop1" src="https://static.igem.org/mediawiki/2011/c/cf/UCalgary_MainPageBox1.png">
 +
<div id="box1pwrap">
 +
<p>
 +
This year, the University of Calgary iGEM team aims to build a biosensor for naphthenic acids (NAs). NAs are organic acids that are toxic to terrestrial and aquatic wildlife. Because they are waste products of the bitumen extraction process that mines petroleum from the Alberta oil sands, the accumulation of NAs in the on-site settling ponds (tailings ponds) is an environmental concern requiring constant monitoring and eventual remediation. In addition to harming the environment, NAs also contribute to refinery equipment corrosion, directly increasing maintenance and replacement costs. Currently, sophisticated and expensive procedures, such as Fourier Transform Infrared Spectrometer (FTIR) or gas chromatography-mass spectrometry, are needed to identify and assess NA concentrations in the environment. A bacterial sensor, if developed, could greatly improve the cost- and time-efficiency of NA detection, and facilitate a workable approach for remediation.
 +
</p>
 +
</div>
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</div>
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<div id="box4">
 +
<div id="subdefault">
 +
<h3>Our Naphthenic Acid Biosensor</h3>
 +
<p><br><br>A detailed description of our project can be seen <a style="color:#42ffd9;" href="https://2011.igem.org/Team:Calgary/Project">here</a>! Below is a picture of what our final product will look like. We will have our biosensing bacterium in a container that the sample to be measured can be added to.</p><br><br><p style="font-size: 14px;"><b>Roll over the bacterium below!</b></p>
 +
</div>
 +
 +
<div id="subpromoter">
 +
<h3>An NA-Sensitive Promoter</h3>
 +
<p>The most critical component of our biosensor is a naphthenic acid sensory element. This would likely be a promoter element. The major challenge for this was that given the lack of available information on NA degradation, there was no NA sensitive promoter available in the literature for us to use. We needed to develop methods to search for such an element. These included our novel “fishing” method as well as our bioinformatics approach. Click on the promoter to learn more about this!</p>
 +
</div>
 +
 +
<div id="subreporter">
 +
<h3>An Electrochemical Reporter</h3>
 +
<p>We need a reliable reporter for our system. Given the highly variable and often turbid nature of oil sands tailings ponds however, we needed a special kind of reporter. We chose to use the <i>lacZ</i> gene, but characterize it as an electrochemical reporter. Click the gene to learn more!</p>
 +
</div>
 +
 +
<div id="subchassis">
 +
<h3>A Suitable Chassis</h3>
 +
<p>We need a chassis in which to house our system. Given that we intended to get our sensory element from either <i>Pseudomonas</i> or microalgae, and that these organisms are both naturally present within tailings ponds, we chose to focus on them. As such, we contributed and characterized several parts for future work in microalgae, and to move plasmids efficiently from <i>E. coli</i> to <i>Pseudomonas</i>. Click the chassis to learn more!</p>
 +
</div>
 +
</div>
 +
 +
<div id="box2">
 +
<h2>The Vision: A Naphthenic Acid Biosensor</h2>
 +
<p>We plan to create a relatively small, cheap biosensing device that can respond to naphthenic acids and produce an electrochemical output. Roll over the individual parts for more details.</p>
 +
 +
<img id="sensorimage" src="https://static.igem.org/mediawiki/2011/f/f8/UCalgary2011_MainPageDevice.png">
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<h2>Our Sponsors</h2>
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<td><a href="http://www.neb.com/nebecomm/default.asp"><img src="https://static.igem.org/mediawiki/2011/d/d6/UCalgary_NEB_Logo.png"></a></td>
 +
<td><a href="http://www.ucalgary.ca/bhsc/"><img src="https://static.igem.org/mediawiki/2011/c/c7/UCalgary_BHSc_Logo.png"></a></td>
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<td><a href="http://www.vwr.com/"><img src="https://static.igem.org/mediawiki/2011/6/65/UCalgary_VWR_Logo.png"></a></td>
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<td><a href="http://www.nexeninc.com/"><img src="https://static.igem.org/mediawiki/2011/a/a8/UCalgary_Nexen_Logo.png"></a></td>
 +
<td><a href="http://www.sarstedt.com/php/main.php"><img src="https://static.igem.org/mediawiki/2011/e/ee/UCalgary_Sarstedt_Logo.png"></a></td>
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<td><a href="http://www.sigmaaldrich.com/canada-english.html?cm_mmc=Google_eBusiness-_-search-_-SIAL+Countries_Countries-_-sigma+aldrich+canada_BROAD&amp;cm_guid=1-_-100000000000000025748-_-7408517799"><img src="https://static.igem.org/mediawiki/2011/8/8a/UCalgary_Sigma_Aldrich_Logo.png"></a></td>
 +
<td><a href="http://www.mhc.ab.ca/"><img src="https://static.igem.org/mediawiki/2011/7/78/UCalgary2011_Medicine_Hat_College_Logo.png"></a></td>
 +
</tr>
 +
</tbody></table>
 +
<img class="boxbottom3" src="https://static.igem.org/mediawiki/2011/6/6c/UCalgary_MainPageBox3Bottom.png">
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</script><div class="twtr-widget twtr-widget-profile twtr-scroll" id="twtr-widget-1"><div class="twtr-doc" style="width: 250px;">            <div class="twtr-hd"><a target="_blank" href="https://twitter.com/intent/user?screen_name=iGEMCalgary" class="twtr-profile-img-anchor"><img alt="profile" class="twtr-profile-img" src="http://a0.twimg.com/profile_images/2200521906/Team_Logo_Updated_Twitter_normal.png"></a>                      <h3>iGEM Calgary</h3>                      <h4><a target="_blank" href="https://twitter.com/intent/user?screen_name=iGEMCalgary">iGEMCalgary</a></h4>            </div>            <div class="twtr-bd">              <div class="twtr-timeline" style="height: 300px;">                <div class="twtr-tweets">                  <div class="twtr-reference-tweet"></div><div class="twtr-tweet" id="tweet-id-5"><div class="twtr-tweet-wrap">        <div class="twtr-avatar">          <div class="twtr-img"><a target="_blank" href="https://twitter.com/intent/user?screen_name=iGEMCalgary"><img alt="iGEMCalgary profile" src="http://a0.twimg.com/profile_images/2200521906/Team_Logo_Updated_Twitter_normal.png"></a></div>        </div>        <div class="twtr-tweet-text">          <p>            <a target="_blank" href="https://twitter.com/intent/user?screen_name=iGEMCalgary" class="twtr-user">iGEMCalgary</a> <a class="tweet-url username" data-screen-name="UCLiGEM" href="http://twitter.com/UCLiGEM" target="_blank">@UCLiGEM</a> Google should have a massive repository of fonts online that you can directly reference in your CSS. Tried searching for it?            <em>            <a target="_blank" class="twtr-timestamp" time="Tue Jun 12 05:50:45 +0000 2012" href="https://twitter.com/iGEMCalgary/status/212421664149995521">2 days ago</a> ·            <a target="_blank" class="twtr-reply" href="https://twitter.com/intent/tweet?in_reply_to=212421664149995521">reply</a> ·            <a target="_blank" class="twtr-rt" href="https://twitter.com/intent/retweet?tweet_id=212421664149995521">retweet</a> ·            <a target="_blank" class="twtr-fav" href="https://twitter.com/intent/favorite?tweet_id=212421664149995521">favorite</a>            </em>          </p>        </div>      </div></div><div class="twtr-tweet" id="tweet-id-4"><div class="twtr-tweet-wrap">        <div class="twtr-avatar">          <div class="twtr-img"><a target="_blank" href="https://twitter.com/intent/user?screen_name=iGEMCalgary"><img alt="iGEMCalgary profile" src="http://a0.twimg.com/profile_images/2200521906/Team_Logo_Updated_Twitter_normal.png"></a></div>        </div>        <div class="twtr-tweet-text">          <p>            <a target="_blank" href="https://twitter.com/intent/user?screen_name=iGEMCalgary" class="twtr-user">iGEMCalgary</a> <a class="tweet-url username" data-screen-name="Nesciosquid" href="http://twitter.com/Nesciosquid" target="_blank">@Nesciosquid</a> Sadly, he isn't on the team this year, but we will be certain to pass on the message!            <em>            <a target="_blank" class="twtr-timestamp" time="Mon May 28 00:19:49 +0000 2012" href="https://twitter.com/iGEMCalgary/status/206902562056716289">17 days ago</a> ·            <a target="_blank" class="twtr-reply" href="https://twitter.com/intent/tweet?in_reply_to=206902562056716289">reply</a> ·            <a target="_blank" class="twtr-rt" href="https://twitter.com/intent/retweet?tweet_id=206902562056716289">retweet</a> ·            <a target="_blank" class="twtr-fav" href="https://twitter.com/intent/favorite?tweet_id=206902562056716289">favorite</a>            </em>          </p>        </div>      </div></div><div class="twtr-tweet" id="tweet-id-3"><div class="twtr-tweet-wrap">        <div class="twtr-avatar">          <div class="twtr-img"><a target="_blank" href="https://twitter.com/intent/user?screen_name=iGEMCalgary"><img alt="iGEMCalgary profile" src="http://a0.twimg.com/profile_images/2200521906/Team_Logo_Updated_Twitter_normal.png"></a></div>        </div>        <div class="twtr-tweet-text">          <p>            <a target="_blank" href="https://twitter.com/intent/user?screen_name=iGEMCalgary" class="twtr-user">iGEMCalgary</a> Great iGEM workshop this weekend by <a class="tweet-url username" data-screen-name="ABInnovates" href="http://twitter.com/ABInnovates" target="_blank">@ABInnovates</a>, featuring great guest speakers! Entrepreneurial bootcamp by <a class="tweet-url username" data-screen-name="venturealberta" href="http://twitter.com/venturealberta" target="_blank">@venturealberta</a> was fantastic!            <em>            <a target="_blank" class="twtr-timestamp" time="Sun May 27 21:26:37 +0000 2012" href="https://twitter.com/iGEMCalgary/status/206858973897437184">18 days ago</a> ·            <a target="_blank" class="twtr-reply" href="https://twitter.com/intent/tweet?in_reply_to=206858973897437184">reply</a> ·            <a target="_blank" class="twtr-rt" href="https://twitter.com/intent/retweet?tweet_id=206858973897437184">retweet</a> ·            <a target="_blank" class="twtr-fav" href="https://twitter.com/intent/favorite?tweet_id=206858973897437184">favorite</a>            </em>          </p>        </div>      </div></div><div class="twtr-tweet" id="tweet-id-2"><div class="twtr-tweet-wrap">        <div class="twtr-avatar">          <div class="twtr-img"><a target="_blank" href="https://twitter.com/intent/user?screen_name=Genomengin"><img alt="Genomengin profile" src="http://a0.twimg.com/profile_images/1079723996/twit_normal.jpg"></a></div>        </div>        <div class="twtr-tweet-text">          <p>            <a target="_blank" href="https://twitter.com/intent/user?screen_name=Genomengin" class="twtr-user">Genomengin</a> Four bases in RNA? Think again… <a href="http://t.co/RvkKcw0Y" target="_blank" urlentities="[object Object]" rel="nofollow">nblo.gs/xPD56</a>            <em>            <a target="_blank" class="twtr-timestamp" time="Sat May 19 11:22:57 +0000 2012" href="https://twitter.com/Genomengin/status/203807953529225217">26 days ago</a> ·            <a target="_blank" class="twtr-reply" href="https://twitter.com/intent/tweet?in_reply_to=203807953529225217">reply</a> ·            <a target="_blank" class="twtr-rt" href="https://twitter.com/intent/retweet?tweet_id=203807953529225217">retweet</a> ·            <a target="_blank" class="twtr-fav" href="https://twitter.com/intent/favorite?tweet_id=203807953529225217">favorite</a>            </em>          </p>        </div>      </div></div><div class="twtr-tweet" id="tweet-id-1"><div class="twtr-tweet-wrap">        <div class="twtr-avatar">          <div class="twtr-img"><a target="_blank" href="https://twitter.com/intent/user?screen_name=ewaltersi"><img alt="ewaltersi profile" src="http://a0.twimg.com/profile_images/1783406106/iGEM_worlds_small_normal.png"></a></div>        </div>        <div class="twtr-tweet-text">          <p>            <a target="_blank" href="https://twitter.com/intent/user?screen_name=ewaltersi" class="twtr-user">ewaltersi</a> Free stickers, pins, &amp; bubble wrap? Awwww yeah, the <a class="tweet-url username" data-screen-name="iGEM" href="http://twitter.com/iGEM" target="_blank">@iGEM</a> distribution is here! (And some cool DNA too, I guess)            <em>            <a target="_blank" class="twtr-timestamp" time="Wed May 16 21:00:39 +0000 2012" href="https://twitter.com/ewaltersi/status/202866174550228994">29 days ago</a> ·            <a target="_blank" class="twtr-reply" href="https://twitter.com/intent/tweet?in_reply_to=202866174550228994">reply</a> ·            <a target="_blank" class="twtr-rt" href="https://twitter.com/intent/retweet?tweet_id=202866174550228994">retweet</a> ·            <a target="_blank" class="twtr-fav" href="https://twitter.com/intent/favorite?tweet_id=202866174550228994">favorite</a>            </em>          </p>        </div>      </div></div>                  <!-- tweets show here -->                </div>              </div>            </div>            <div class="twtr-ft">              <div><a target="_blank" href="https://twitter.com"><img alt="" src="//widgets.twimg.com/i/widget-bird.png"></a>                <span><a target="_blank" class="twtr-join-conv" style="color:#ffffff" href="https://twitter.com/iGEMCalgary">Join the conversation</a></span>              </div>            </div>          </div></div>
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{|align="justify"
{|align="justify"
|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.

Revision as of 22:17, 14 June 2012

Team:Calgary - 2011.igem.org

Team:Calgary

From 2011.igem.org

Welcome!

This year, the University of Calgary iGEM team aims to build a biosensor for naphthenic acids (NAs). NAs are organic acids that are toxic to terrestrial and aquatic wildlife. Because they are waste products of the bitumen extraction process that mines petroleum from the Alberta oil sands, the accumulation of NAs in the on-site settling ponds (tailings ponds) is an environmental concern requiring constant monitoring and eventual remediation. In addition to harming the environment, NAs also contribute to refinery equipment corrosion, directly increasing maintenance and replacement costs. Currently, sophisticated and expensive procedures, such as Fourier Transform Infrared Spectrometer (FTIR) or gas chromatography-mass spectrometry, are needed to identify and assess NA concentrations in the environment. A bacterial sensor, if developed, could greatly improve the cost- and time-efficiency of NA detection, and facilitate a workable approach for remediation.

Our Naphthenic Acid Biosensor



A detailed description of our project can be seen here! Below is a picture of what our final product will look like. We will have our biosensing bacterium in a container that the sample to be measured can be added to.



Roll over the bacterium below!

An NA-Sensitive Promoter

The most critical component of our biosensor is a naphthenic acid sensory element. This would likely be a promoter element. The major challenge for this was that given the lack of available information on NA degradation, there was no NA sensitive promoter available in the literature for us to use. We needed to develop methods to search for such an element. These included our novel “fishing” method as well as our bioinformatics approach. Click on the promoter to learn more about this!

An Electrochemical Reporter

We need a reliable reporter for our system. Given the highly variable and often turbid nature of oil sands tailings ponds however, we needed a special kind of reporter. We chose to use the lacZ gene, but characterize it as an electrochemical reporter. Click the gene to learn more!

A Suitable Chassis

We need a chassis in which to house our system. Given that we intended to get our sensory element from either Pseudomonas or microalgae, and that these organisms are both naturally present within tailings ponds, we chose to focus on them. As such, we contributed and characterized several parts for future work in microalgae, and to move plasmids efficiently from E. coli to Pseudomonas. Click the chassis to learn more!

The Vision: A Naphthenic Acid Biosensor

We plan to create a relatively small, cheap biosensing device that can respond to naphthenic acids and produce an electrochemical output. Roll over the individual parts for more details.

Promoter Reporter Chassis

Our Sponsors

profile

iGEM Calgary

iGEMCalgary

iGEMCalgary profile

iGEMCalgary @UCLiGEM Google should have a massive repository of fonts online that you can directly reference in your CSS. Tried searching for it? 2 days ago · reply · retweet · favorite

iGEMCalgary profile

iGEMCalgary @Nesciosquid Sadly, he isn't on the team this year, but we will be certain to pass on the message! 17 days ago · reply · retweet · favorite

iGEMCalgary profile

iGEMCalgary Great iGEM workshop this weekend by @ABInnovates, featuring great guest speakers! Entrepreneurial bootcamp by @venturealberta was fantastic! 18 days ago · reply · retweet · favorite

Genomengin profile

Genomengin Four bases in RNA? Think again… nblo.gs/xPD56 26 days ago · reply · retweet · favorite

ewaltersi profile

ewaltersi Free stickers, pins, & bubble wrap? Awwww yeah, the @iGEM distribution is here! (And some cool DNA too, I guess) 29 days ago · reply · retweet · favorite

You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.
Colorado State logo.png

Tell us more about your project. Give us background. Use this as the abstract of your project. Be descriptive but concise (1-2 paragraphs)

Your team picture
Team Colorado_State


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