Team:Colorado State

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This year, the University of Calgary iGEM team aims to build a biosensor for naphthenic acids (NAs). NAs are organic acids that are toxic to terrestrial and aquatic wildlife. Because they are waste products of the bitumen extraction process that mines petroleum from the Alberta oil sands, the accumulation of NAs in the on-site settling ponds (tailings ponds) is an environmental concern requiring constant monitoring and eventual remediation. In addition to harming the environment, NAs also contribute to refinery equipment corrosion, directly increasing maintenance and replacement costs. Currently, sophisticated and expensive procedures, such as Fourier Transform Infrared Spectrometer (FTIR) or gas chromatography-mass spectrometry, are needed to identify and assess NA concentrations in the environment. A bacterial sensor, if developed, could greatly improve the cost- and time-efficiency of NA detection, and facilitate a workable approach for remediation.
 
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<h3>Our Naphthenic Acid Biosensor</h3>
 
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<p><br><br>A detailed description of our project can be seen <a style="color:#42ffd9;" href="http://2011.igem.org/Team:Calgary/Project">here</a>! Below is a picture of what our final product will look like. We will have our biosensing bacterium in a container that the sample to be measured can be added to.</p><br><br><p style="font-size: 14px;"><b>Roll over the bacterium below!</b></p>
 
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<h3>An NA-Sensitive Promoter</h3>
 
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<p>The most critical component of our biosensor is a naphthenic acid sensory element. This would likely be a promoter element. The major challenge for this was that given the lack of available information on NA degradation, there was no NA sensitive promoter available in the literature for us to use. We needed to develop methods to search for such an element. These included our novel “fishing” method as well as our bioinformatics approach. Click on the promoter to learn more about this!</p>
 
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<h3>An Electrochemical Reporter</h3>
 
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<p>We need a reliable reporter for our system. Given the highly variable and often turbid nature of oil sands tailings ponds however, we needed a special kind of reporter. We chose to use the <i>lacZ</i> gene, but characterize it as an electrochemical reporter. Click the gene to learn more!</p>
 
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<h3>A Suitable Chassis</h3>
 
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<p>We need a chassis in which to house our system. Given that we intended to get our sensory element from either <i>Pseudomonas</i> or microalgae, and that these organisms are both naturally present within tailings ponds, we chose to focus on them. As such, we contributed and characterized several parts for future work in microalgae, and to move plasmids efficiently from <i>E. coli</i> to <i>Pseudomonas</i>. Click the chassis to learn more!</p>
 
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<h2>The Vision: A Naphthenic Acid Biosensor</h2>
 
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<p>We plan to create a relatively small, cheap biosensing device that can respond to naphthenic acids and produce an electrochemical output. Roll over the individual parts for more details.</p>
 
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<img id="sensorimage" src="http://2011.igem.org/wiki/images/f/f8/UCalgary2011_MainPageDevice.png"></img>
 
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<img id="pulloutbox" src="http://2011.igem.org/wiki/images/b/b4/UCalgary2011_MainPageBacterialZoomBox.png"></img>
 
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<img id="circuit" src="http://2011.igem.org/wiki/images/8/80/UCalgary2011_BacterialCircuitBase.png"></img>
 
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<a id="promoter" href="http://2011.igem.org/Team:Calgary/Project/Promoter">Promoter</a>
 
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<a id="reporter" href="http://2011.igem.org/Team:Calgary/Project/Reporter">Reporter</a>
 
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<a id="chassis" href="http://2011.igem.org/Team:Calgary/Project/Chassis">Chassis</a>
 
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<h2>Our Sponsors</h2>
 
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<td><a href="http://www.ucalgary.ca/"><img src="http://2011.igem.org/wiki/images/c/c1/UCalgary_UofCLogo.png"></img></a></td>
 
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<td><a href="http://www.albertatechfutures.ca/"><img src="http://2011.igem.org/wiki/images/3/3f/UCalgary2011_AITF_Logo_Small.png"></img></a></td>
 
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<td><a href="http://www.osli.ca/"><img style="width: 510px;" src="http://2011.igem.org/wiki/images/d/da/UCalgary2011_OSLI_Banner_Logo_Small.png"></img></a></td>
 
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<td><a href="http://www.osrin.ualberta.ca/"><img src="http://2011.igem.org/wiki/images/9/9a/UCalgary_OSRIN_Logo.png"></img></a></td>
 
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<td><a href="http://www.neb.com/nebecomm/default.asp"><img src="http://2011.igem.org/wiki/images/d/d6/UCalgary_NEB_Logo.png"></img></a></td>
 
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<td><a href="http://www.ucalgary.ca/bhsc/"><img src="http://2011.igem.org/wiki/images/c/c7/UCalgary_BHSc_Logo.png"></img></a></td>
 
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<td><a href="http://www.vwr.com/"><img src="http://2011.igem.org/wiki/images/6/65/UCalgary_VWR_Logo.png"></img></a></td>
 
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<td><a href="http://www.nexeninc.com/"><img src="http://2011.igem.org/wiki/images/a/a8/UCalgary_Nexen_Logo.png"></img></a></td>
 
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<td><a href="http://www.sarstedt.com/php/main.php"><img src="http://2011.igem.org/wiki/images/e/ee/UCalgary_Sarstedt_Logo.png"></a></img></td>
 
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<td><a href="http://www.sigmaaldrich.com/canada-english.html?cm_mmc=Google_eBusiness-_-search-_-SIAL+Countries_Countries-_-sigma+aldrich+canada_BROAD&cm_guid=1-_-100000000000000025748-_-7408517799"><img src="http://2011.igem.org/wiki/images/8/8a/UCalgary_Sigma_Aldrich_Logo.png"></img></a></td>
 
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<td><a href="http://www.mhc.ab.ca/"><img src="http://2011.igem.org/wiki/images/7/78/UCalgary2011_Medicine_Hat_College_Logo.png"></img></a></td>
 
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.

Revision as of 22:15, 14 June 2012

You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.
Colorado State logo.png

Tell us more about your project. Give us background. Use this as the abstract of your project. Be descriptive but concise (1-2 paragraphs)

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Team Colorado_State


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