Team:Colorado State

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This year, the University of Calgary iGEM team aims to build a biosensor for naphthenic acids (NAs). NAs are organic acids that are toxic to terrestrial and aquatic wildlife. Because they are waste products of the bitumen extraction process that mines petroleum from the Alberta oil sands, the accumulation of NAs in the on-site settling ponds (tailings ponds) is an environmental concern requiring constant monitoring and eventual remediation. In addition to harming the environment, NAs also contribute to refinery equipment corrosion, directly increasing maintenance and replacement costs. Currently, sophisticated and expensive procedures, such as Fourier Transform Infrared Spectrometer (FTIR) or gas chromatography-mass spectrometry, are needed to identify and assess NA concentrations in the environment. A bacterial sensor, if developed, could greatly improve the cost- and time-efficiency of NA detection, and facilitate a workable approach for remediation.
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<h3>Our Naphthenic Acid Biosensor</h3>
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<p><br><br>A detailed description of our project can be seen <a style="color:#42ffd9;" href="https://2011.igem.org/Team:Calgary/Project">here</a>! Below is a picture of what our final product will look like. We will have our biosensing bacterium in a container that the sample to be measured can be added to.</p><br><br><p style="font-size: 14px;"><b>Roll over the bacterium below!</b></p>
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<a class="editbutton" href="https://2012.igem.org/wiki/index.php?title=Team:Colorado_State&amp;action=edit">Edit page</a>
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<h3>An NA-Sensitive Promoter</h3>
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<h1>Our Gluten-Free Mission</h1>
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<p>The most critical component of our biosensor is a naphthenic acid sensory element. This would likely be a promoter element. The major challenge for this was that given the lack of available information on NA degradation, there was no NA sensitive promoter available in the literature for us to use. We needed to develop methods to search for such an element. These included our novel “fishing” method as well as our bioinformatics approach. Click on the promoter to learn more about this!</p>
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<h3>An Electrochemical Reporter</h3>
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<p>We need a reliable reporter for our system. Given the highly variable and often turbid nature of oil sands tailings ponds however, we needed a special kind of reporter. We chose to use the <i>lacZ</i> gene, but characterize it as an electrochemical reporter. Click the gene to learn more!</p>
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<h3>A Suitable Chassis</h3>
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<p>We need a chassis in which to house our system. Given that we intended to get our sensory element from either <i>Pseudomonas</i> or microalgae, and that these organisms are both naturally present within tailings ponds, we chose to focus on them. As such, we contributed and characterized several parts for future work in microalgae, and to move plasmids efficiently from <i>E. coli</i> to <i>Pseudomonas</i>. Click the chassis to learn more!</p>
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<img src="https://lh6.googleusercontent.com/-cUic9qTY128/UA3HlblblkI/AAAAAAAAATI/m2Iu9XgpMFM/s512/gluten_free_keg.png" width="250" style="border: none; float: right; margin-left: 15px;" />
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<h2>The Vision: A Naphthenic Acid Biosensor</h2>
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<p>We plan to create a relatively small, cheap biosensing device that can respond to naphthenic acids and produce an electrochemical output. Roll over the individual parts for more details.</p>
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<a id="promoter" href="https://2011.igem.org/Team:Calgary/Project/Promoter">Promoter</a>
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<a id="reporter" href="https://2011.igem.org/Team:Calgary/Project/Reporter">Reporter</a>
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<a id="chassis" href="https://2011.igem.org/Team:Calgary/Project/Chassis">Chassis</a>
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<p>If you have Celiac Disease or are Gluten-Intolerant (and are over 21), stop what you are doing right now. Your prayers for a great tasting gluten-free beer have finally been answered, or at least are in the process of being answered. Think of it as a delay in the mailroom. All of those nights when your friends were taunting you with the ice cold, delicious, golden liquid that gives courage to even the most faint of heart are quickly coming to an end. Your desire to enjoy a "cold one" without having a gut-wrenching reaction should be fullfilled by the end of the summer. The CSU iGEM team is working on producing a "gluten-free" beer that can be brewed from wheat or barley so you can savor that same great taste you know and love. So cross your fingers, bust out your lucky rabbits foot, and watch out for black cats because we are gearing up for something big, and it has never been done before.</p>
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<h1>More Than a Great Beer</h1>
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<h2>Our Sponsors</h2>
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<td><a href="http://www.ucalgary.ca/"><img src="https://static.igem.org/mediawiki/2011/c/c1/UCalgary_UofCLogo.png"></img></a></td>
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<td><a href="http://www.albertatechfutures.ca/"><img src="https://static.igem.org/mediawiki/2011/3/3f/UCalgary2011_AITF_Logo_Small.png"></img></a></td>
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<td><a href="http://www.neb.com/nebecomm/default.asp"><img src="https://static.igem.org/mediawiki/2011/d/d6/UCalgary_NEB_Logo.png"></img></a></td>
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<td><a href="http://www.ucalgary.ca/bhsc/"><img src="https://static.igem.org/mediawiki/2011/c/c7/UCalgary_BHSc_Logo.png"></img></a></td>
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<td><a href="http://www.nexeninc.com/"><img src="https://static.igem.org/mediawiki/2011/a/a8/UCalgary_Nexen_Logo.png"></img></a></td>
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<td><a href="http://www.sarstedt.com/php/main.php"><img src="https://static.igem.org/mediawiki/2011/e/ee/UCalgary_Sarstedt_Logo.png"></a></img></td>
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<td><a href="http://www.mhc.ab.ca/"><img src="https://static.igem.org/mediawiki/2011/7/78/UCalgary2011_Medicine_Hat_College_Logo.png"></img></a></td>
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<p>A gluten-free beer isn't the only thing we are working on. We are also designing primers for New Belgium Brewery, developing a novel gluten-detecting assay, and creating a computer-modeled simulation for the breakdown of gluten in beer. All of these various components have us teaming up with some of the best in the business, so don't forget to visit our sponsor and attribution pages.</p>
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<h1>RamStrong</h1>
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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''Tell us more about your project. Give us background.  Use this as the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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|align="center"|[[Team:Colorado_State | Team Colorado_State]]
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<!--- The Mission, Experiments --->
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<p>We proudly roam the steepest and most treacherous mountain sides, looking for adventure, and exploring whatever comes our way. We do not back down from challenges, instead we charge them head on. Although we do occasionally clash heads, we power through our disagreements, because... we are the CSU Rams!!!</p>
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!align="center"|[[Team:Colorado_State|Home]]
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!align="center"|[[Team:Colorado_State/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2012&team_name=Colorado_State Official Team Profile]
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!align="center"|[[Team:Colorado_State/Project|Project]]
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!align="center"|[[Team:Colorado_State/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Colorado_State/Modeling|Modeling]]
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!align="center"|[[Team:Colorado_State/Notebook|Notebook]]
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Latest revision as of 06:51, 26 June 2013

Edit page

Our Gluten-Free Mission

If you have Celiac Disease or are Gluten-Intolerant (and are over 21), stop what you are doing right now. Your prayers for a great tasting gluten-free beer have finally been answered, or at least are in the process of being answered. Think of it as a delay in the mailroom. All of those nights when your friends were taunting you with the ice cold, delicious, golden liquid that gives courage to even the most faint of heart are quickly coming to an end. Your desire to enjoy a "cold one" without having a gut-wrenching reaction should be fullfilled by the end of the summer. The CSU iGEM team is working on producing a "gluten-free" beer that can be brewed from wheat or barley so you can savor that same great taste you know and love. So cross your fingers, bust out your lucky rabbits foot, and watch out for black cats because we are gearing up for something big, and it has never been done before.

More Than a Great Beer

A gluten-free beer isn't the only thing we are working on. We are also designing primers for New Belgium Brewery, developing a novel gluten-detecting assay, and creating a computer-modeled simulation for the breakdown of gluten in beer. All of these various components have us teaming up with some of the best in the business, so don't forget to visit our sponsor and attribution pages.






RamStrong

We proudly roam the steepest and most treacherous mountain sides, looking for adventure, and exploring whatever comes our way. We do not back down from challenges, instead we charge them head on. Although we do occasionally clash heads, we power through our disagreements, because... we are the CSU Rams!!!



Retrieved from "http://2012.igem.org/Team:Colorado_State"