Team:Colombia/Parts

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Contents

Parts

Toxin- Antitoxin (TA) Modules

Our Basic Parts

TA BioBricks1-2.png


Our Composite Parts

We send all this parts to the Registry!!!!

HipA7 TisB HipB MqsA istR

prm-hipA7 plac-hipB ptetR-mqsR plac-istR

Ralstonia:

Ralstoniaparts.png


pchS: It encodes a histidine kinase sensor of a two-component signal transduction system. Responds to threshold concentrations of the Quorum Sensing unusual signal 3-OH PAME.

pchR: Receptor of a two-component signal transduction system. Responds to threshold concentrations of the Quorum Sensing unusual signal 3-OH PAME. It is an atypical response regulator that has a histidine kinase as its output domain. Autophosphorilation after 3OH-PAME recognition may have en effect in phcA activation.

pchA:LysR-type transcriptional regulator that controls expression of many genes and is induced in a density manner, relying on the unusual Quorum Sensing signal 3OH-PAME.

PxpsR: Promoter region of xpsR (PxpsR) wich integrates different signals including phcA. Expression of genes regulated by XpsR may be correlated with threshold concentrations of 3 OH-PAME.

PxpsR-eYFP: Fluorescent Reporter to asses the response of PxpsR to different concentrations of 3OH-PAME.

Aliivibrio fischeri

Alis.png

The overall model can be seen in figures in the main page of Aliivibrio/Streptomyces.

chiP: Chitoporin is a specific channel that allows N-acetylglucosamine dimers to cross the outer membrane to the periplasmic space,

chiA: Chitinase is an hydrolase that cleaves the glycosidic bonds on the chitin polymer.

chiS: The histidine-kinase sensor is part of a two-component signalling system, that activates a promoter, in this case pChitin.

cbp: Chitin-binding protein is able to bind the N-acetyl glucosamine dimers in the periplasmic space. The complex CBP+(GlcNAc)2 serves as an activator of the signalling from chiS to the promoter.

PChitin: This 100bp region upstream of chiP is thought to be activated by the activation of chiS.

Alert System:

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