Team:Colombia/Parts

From 2012.igem.org

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== Team Colombia @ 2012 iGEM ==
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{{https://2012.igem.org/User:Tabima}}
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<html xmlns="http://www.w3.org/1999/xhtml">
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<div class="right_box">
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<head>
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<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" />
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<style type="text/css">
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/* These styles just pretty up the page a bit. */
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html
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{
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overflow: -moz-scrollbars-vertical !important;
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resize: none;
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background-color: #f5f6f7;
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#top-section {
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== '''Parts''' ==
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height: 0px;
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border-top: 1
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border-left: none;
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border-right: none;
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}
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#siteSub {
 
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display:none;
 
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}
 
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#search-controls {
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== '''Toxin- Antitoxin (TA) Modules''' ==
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display:none;
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}
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.firstHeading {
 
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display:none;
 
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}
 
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#search-controls {
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== Our Basic Parts ==
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margin-top:0px;
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}
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#footer-box {
 
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display:none;
 
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}
 
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body
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[[File:TA_BioBricks1-2.png|center|550px]]
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{
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background-color: #f5f6f7;
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}
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body {
 
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  font: 65%/1.2 Arial, Helvetica, sans-serif;
 
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  background-color: #eee; }
 
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#wrap {
 
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  font-size: 0.7em;
 
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  width: 960px;
 
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  padding: 0px;
 
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  margin: 0 auto;
 
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  background-color: #fff;
 
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  position: relative; }
 
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/* These styles create the dropdown menus. */
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== Our Composite Parts ==
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#navbar {
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  margin: 1600;
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  padding: 0;
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  height: 1em; }
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#navbar li {
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  list-style: none;
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  float: left; }
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#navbar li a {
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  padding-left: 19px;
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  padding-bottom: 3px;
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  padding-right: 8px;
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  padding-top: 3px;
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  display: block;
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  background-color: #5d9548;
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  color: #fff;
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  text-decoration: none; }
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#navbar li ul {
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  display: none;
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  width: 10em; /* Width to help Opera out */
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  background-color: #80ff00;}
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#navbar li:hover ul, #navbar li.hover ul {
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  display: block;
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  position: absolute;
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  background-color: #5d9549;
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  padding: 0; }
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#navbar li:hover li, #navbar li.hover li {
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  float: none; }
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#navbar li:hover li a, #navbar li.hover li a {
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  background-color: #66b83d;
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  border-bottom: 1px solid #fff;
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  color: white; }
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#navbar li li a:hover {
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  background-color: #8db3ff; }
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</style>
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<script>
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[[File:TA_BioBricks3.png|center|550px]]
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// Javascript originally by Patrick Griffiths and Dan Webb.
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// http://htmldog.com/articles/suckerfish/dropdowns/
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sfHover = function() {
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  var sfEls = document.getElementById("navbar").getElementsByTagName("li");
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  for (var i=0; i<sfEls.length; i++) {
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      sfEls[i].onmouseover=function() {
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        this.className+=" hover";
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      }
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      sfEls[i].onmouseout=function() {
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        this.className=this.className.replace(new RegExp(" hover\\b"), "");
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  }
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}
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if (window.attachEvent) window.attachEvent("onload", sfHover);
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</script>
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</head>
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='''We send all this parts to the Registry!!!! '''=
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<body>
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  <div id="wrap">
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      <ul id="navbar">
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[http://partsregistry.org/Part:BBa_K831000:Design HipA7]
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        <li><a href="https://2012.igem.org/Team:Colombia">Home</a>
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[http://partsregistry.org/Part:BBa_K831003:Design TisB]
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        </li>
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[http://partsregistry.org/Part:BBa_K831001:Design HipB]
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        <li><a href="https://2012.igem.org/Team:Colombia/Team">The Team</a>
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[http://partsregistry.org/Part:BBa_K831002:Design MqsA]
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            <ul>
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[http://partsregistry.org/Part:BBa_K831004:Design ''istR'' ]
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              <li><a href="https://2012.igem.org/Team:Colombia/Team#The_Team">The Team</a></li>
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              <li><a href="https://2012.igem.org/Team:Colombia/Team#Undergrads">Undergrads</a></li>
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              <li><a href="https://2012.igem.org/Team:Colombia/Team#Grad_students_.28Also_known_as_the_Aging_ones.21.29">Grad Students</a></li>
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              <li><a href="https://2012.igem.org/Team:Colombia/Team#Instructors:">Instructors</a></li>
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            </ul>
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        </li>
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        <li><a href="https://2012.igem.org/Team:Colombia/Project">Project</a>
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            <ul>
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              <li><a href="https://2012.igem.org/Team:Colombia/Project/Basics">The Basics Project</a></li>
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              <li><a href="https://2012.igem.org/Team:Colombia/Project/Problem">The Problem</a></li>
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              <li><a href="https://2012.igem.org/Team:Colombia/Project/Experiments/Pseudomonas">Experiments: Pseudomonas</a></li>
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              <li><a href="https://2012.igem.org/Team:Colombia/Project/Experiments/Ralstonia">Experiments: Ralstonia</a></li>
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              <li><a href="https://2012.igem.org/Team:Colombia/Project/Experiments/Vibrio and Streptomyces">Experiments: Vibrio and Streptomyces</a></li>
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            </ul>       
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        </li>
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        <li><a href="https://2012.igem.org/Team:Colombia/Parts">Parts</a>
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            <!--ul>
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              <li><a href="#">Via Email</a></li>
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              <li><a href="#">Stalk Us Elsewhere</a></li>
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            </ul-->       
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        </li>
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        <li><a href="https://2012.igem.org/Team:Colombia/Modeling">Modeling</a>
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            <ul>
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              <li><a href="https://2012.igem.org/Team:Colombia/Modeling/Diff">Diferential Equations</a></li>
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              <li><a href="https://2012.igem.org/Team:Colombia/Modeling/Paramterers">Parameters</a></li>
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              <li><a href="https://2012.igem.org/Team:Colombia/Modeling/Stochastic">Stochastic Model</a></li>
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            </ul>       
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        </li>
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        <li><a href="https://2012.igem.org/Team:Colombia/Notebook">Results</a>
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        </li>
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        <li><a href="https://2012.igem.org/Team:Colombia/Human">Human Practices</a>
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          <ul>
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              <li><a href="https://2012.igem.org/Team:Colombia/Human/Social: Schools">Social: Schools</a></li>
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              <li><a href="https://2012.igem.org/Team:Colombia/Human/Social: Coffee growers">Social: Coffe growers</a></li>
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              <li><a href="https://2012.igem.org/Team:Colombia/Human/Legislative">Legislative</a></li>
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              <li><a href="https://2012.igem.org/Team:Colombia/Human/IGEMLA">iGEM Latinamerica</a></li>
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            </ul>       
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        </li>
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            <li><a href="https://2012.igem.org/Team:Colombia/Notebook">Notebook</a>
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            <ul>
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              <li><a href="https://2012.igem.org/Team:Colombia/Notebook/Journal">Journal</a></li>
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              <li><a href="https://2012.igem.org/Team:Colombia/Notebook/Meetings">Meetings</a></li>
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              <li><a href="https://2012.igem.org/Team:Colombia/Notebook/Classes">Classes</a></li>
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              <li><a href="https://2012.igem.org/Team:Colombia/Notebook/Protocols">Protocols</a></li>
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              <li><a href="https://2012.igem.org/Team:Colombia/Notebook/Howto">How To: Parts</a></li>
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              <li><a href="https://2012.igem.org/Team:Colombia/Notebook/Human">Human Practices</a></li>
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            </ul>       
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        </li>
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            <li><a href="https://2012.igem.org/Team:Colombia/Safety">Safety</a>
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            <ul>
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              <li><a href="https://2012.igem.org/Team:Colombia/Safety#Safety_Considerations_.22Hazardous_reagents_and_Biohazards.22">Hazard</a></li>
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              <li><a href="https://2012.igem.org/Team:Colombia/Safety#Safety_Considerations_.22Biological_Parts_.26_Devices.22">Biological Parts and Devices</a></li>
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              <li><a href="https://2012.igem.org/Team:Colombia/Safety#Biosafety_Provisions">Biosafety Provisions</a></li>
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            </ul>       
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        </li>
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            <li><a href="https://2012.igem.org/Team:Colombia/Attributions">Attributes</a>
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            <!--ul>
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              <li><a href="#">Bench Press</a></li>
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              <li><a href="#">Military Press</a></li>
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              <li><a href="#">Press n Seal</a></li>
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            </ul-->       
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        </li>
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            <li><a>The University and the Jamboree</a>
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            <ul>
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              <li><a href="http://www.uniandes.edu.co">UniAndes</a></li>
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              <li><a href="https://2012.igem.org/Regions/Latin_America/Jamboree">Latin American Jamboree 2012</a></li>
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            </ul>       
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        </li>
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  <li><a href="https://2012.igem.org/">iGem Homepage</a>
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        </li>
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      </ul>
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  </div>
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</body>
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[http://partsregistry.org/Part:BBa_K831015 prm-''hipA7'']
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</html>
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[http://partsregistry.org/Part:BBa_K831009 plac-''hipB'']
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[http://partsregistry.org/Part:BBa_K831007 ptetR-''mqsR'']
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[http://partsregistry.org/Part:BBa_K831014  plac-''istR'']
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== '''Ralstonia:''' ==
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[[File:Ralstoniaparts.png|center|550px]]
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'''pchS:''' It encodes a histidine kinase sensor of a two-component signal transduction system. Responds to threshold concentrations of the Quorum Sensing unusual signal 3-OH PAME.
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'''pchR:''' Receptor of a two-component signal transduction system. Responds to threshold concentrations of the Quorum Sensing unusual signal 3-OH PAME. It is an atypical response regulator that has a histidine kinase as its output domain. Autophosphorilation after 3OH-PAME recognition may have en effect in phcA activation.
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'''pchA:'''LysR-type transcriptional regulator that controls expression of many genes and is induced in a density manner, relying on the unusual Quorum Sensing signal 3OH-PAME.
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[http://partsregistry.org/Part:BBa_K831016 '''PxpsR:'''] Promoter region of xpsR (PxpsR) wich integrates different signals including phcA. Expression of genes regulated by XpsR may be correlated with threshold concentrations of 3 OH-PAME.
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[http://partsregistry.org/Part:BBa_K831017 '''PxpsR-eYFP:'''] Fluorescent Reporter to asses the response of PxpsR to different concentrations of 3OH-PAME.
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== ''Aliivibrio fischeri'' ==
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[[File:alis.png|center|500px|]]
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The overall model can be seen in figures in [https://2012.igem.org/Team:Colombia/Project/Experiments/Aliivibrio_and_Streptomyces the main page of Aliivibrio/Streptomyces.]
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'''chiP:''' [http://www.ncbi.nlm.nih.gov/gene/3279194 Chitoporin] is a specific channel that allows N-acetylglucosamine dimers to cross the outer membrane to the periplasmic space,
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'''chiA:''' [http://www.ncbi.nlm.nih.gov/gene/3277358 Chitinase] is an hydrolase that cleaves the glycosidic bonds on the chitin polymer.
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'''chiS:''' [http://www.ncbi.nlm.nih.gov/gene/3279287 The histidine-kinase sensor] is part of a two-component signalling system, that activates a promoter, in this case pChitin.
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'''cbp:''' [http://www.ncbi.nlm.nih.gov/gene/3279341 Chitin-binding protein] is able to bind the N-acetyl glucosamine dimers in the periplasmic space. The complex CBP+(GlcNAc)2 serves as an activator of the signalling from chiS to the promoter.
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'''PChitin:''' This 100bp region upstream of chiP is thought to be activated by the activation of chiS.
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== Alert System: ==
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[[File:Cutandpasteparts.png|center|600px]]
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</div>

Latest revision as of 01:42, 27 October 2012

Template:Https://2012.igem.org/User:Tabima

Contents

Parts

Toxin- Antitoxin (TA) Modules

Our Basic Parts

TA BioBricks1-2.png


Our Composite Parts

TA BioBricks3.png

We send all this parts to the Registry!!!!

HipA7 TisB HipB MqsA istR

prm-hipA7 plac-hipB ptetR-mqsR plac-istR

Ralstonia:

Ralstoniaparts.png


pchS: It encodes a histidine kinase sensor of a two-component signal transduction system. Responds to threshold concentrations of the Quorum Sensing unusual signal 3-OH PAME.

pchR: Receptor of a two-component signal transduction system. Responds to threshold concentrations of the Quorum Sensing unusual signal 3-OH PAME. It is an atypical response regulator that has a histidine kinase as its output domain. Autophosphorilation after 3OH-PAME recognition may have en effect in phcA activation.

pchA:LysR-type transcriptional regulator that controls expression of many genes and is induced in a density manner, relying on the unusual Quorum Sensing signal 3OH-PAME.

PxpsR: Promoter region of xpsR (PxpsR) wich integrates different signals including phcA. Expression of genes regulated by XpsR may be correlated with threshold concentrations of 3 OH-PAME.

PxpsR-eYFP: Fluorescent Reporter to asses the response of PxpsR to different concentrations of 3OH-PAME.

Aliivibrio fischeri

Alis.png

The overall model can be seen in figures in the main page of Aliivibrio/Streptomyces.

chiP: Chitoporin is a specific channel that allows N-acetylglucosamine dimers to cross the outer membrane to the periplasmic space,

chiA: Chitinase is an hydrolase that cleaves the glycosidic bonds on the chitin polymer.

chiS: The histidine-kinase sensor is part of a two-component signalling system, that activates a promoter, in this case pChitin.

cbp: Chitin-binding protein is able to bind the N-acetyl glucosamine dimers in the periplasmic space. The complex CBP+(GlcNAc)2 serves as an activator of the signalling from chiS to the promoter.

PChitin: This 100bp region upstream of chiP is thought to be activated by the activation of chiS.

Alert System:

Cutandpasteparts.png
Retrieved from "http://2012.igem.org/Team:Colombia/Parts"