Team:Colombia/Parts

From 2012.igem.org

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== Our Composite Parts ==
== Our Composite Parts ==
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[[File:TA_BioBricks2-2.png|center|550px]]
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[[File:TA_BioBricks3.png|center|550px]]
='''We send all this parts to the Registry!!!! '''=
='''We send all this parts to the Registry!!!! '''=
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'''pchS:'''  
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'''pchS:''' It encodes a histidine kinase sensor of a two-component signal transduction system. Responds to threshold concentrations of the Quorum Sensing unusual signal 3-OH PAME.
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'''pchR:''' Receptor of a two-component signal transduction system. Responds to threshold concentrations of the Quorum Sensing unusual signal 3-OH PAME. It is an atypical response regulator that has a histidine kinase as its output domain. Autophosphorilation after 3OH-PAME recognition may have en effect in phcA activation.
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'''pchR:'''
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'''pchA:'''LysR-type transcriptional regulator that controls expression of many genes and is induced in a density manner, relying on the unusual Quorum Sensing signal 3OH-PAME.
'''pchA:'''LysR-type transcriptional regulator that controls expression of many genes and is induced in a density manner, relying on the unusual Quorum Sensing signal 3OH-PAME.
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'''PxpcR:'''
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[http://partsregistry.org/Part:BBa_K831016 '''PxpsR:'''] Promoter region of xpsR (PxpsR) wich integrates different signals including phcA. Expression of genes regulated by XpsR may be correlated with threshold concentrations of 3 OH-PAME.
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[http://partsregistry.org/Part:BBa_K831017 '''PxpsR-eYFP:'''] Fluorescent Reporter to asses the response of PxpsR to different concentrations of 3OH-PAME.
== ''Aliivibrio fischeri'' ==
== ''Aliivibrio fischeri'' ==
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The overall model can be seen in figures in [http://2012.igem.org/Team:Colombia/Project/Experiments/Aliivibrio_and_Streptomyces the main page of Aliivibrio/Streptomyces.]
The overall model can be seen in figures in [http://2012.igem.org/Team:Colombia/Project/Experiments/Aliivibrio_and_Streptomyces the main page of Aliivibrio/Streptomyces.]
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'''ChiP:''' [http://www.ncbi.nlm.nih.gov/gene/3279194 Chitoporin] is a specific channel that allows N-acetylglucosamine dimers to cross the outer membrane to the periplasmic space,
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'''chiP:''' [http://www.ncbi.nlm.nih.gov/gene/3279194 Chitoporin] is a specific channel that allows N-acetylglucosamine dimers to cross the outer membrane to the periplasmic space,
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'''ChiA:''' [http://www.ncbi.nlm.nih.gov/gene/3277358 Chitinase] is an hydrolase that cleaves the glycosidic bonds on the chitin polymer.  
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'''chiA:''' [http://www.ncbi.nlm.nih.gov/gene/3277358 Chitinase] is an hydrolase that cleaves the glycosidic bonds on the chitin polymer.  
   
   
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'''ChiS:''' [http://www.ncbi.nlm.nih.gov/gene/3279287 The histidine-kinase sensor] is part of a two-component signalling system, that activates a promoter, in this case pChitin.
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'''chiS:''' [http://www.ncbi.nlm.nih.gov/gene/3279287 The histidine-kinase sensor] is part of a two-component signalling system, that activates a promoter, in this case pChitin.
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'''CBP:''' [http://www.ncbi.nlm.nih.gov/gene/3279341 Chitin-binding protein] is able to bind the N-acetyl glucosamine dimers in the periplasmic space. The complex CBP+(GlcNAc)2 serves as an activator of the signalling from chiS to the promoter.
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'''cbp:''' [http://www.ncbi.nlm.nih.gov/gene/3279341 Chitin-binding protein] is able to bind the N-acetyl glucosamine dimers in the periplasmic space. The complex CBP+(GlcNAc)2 serves as an activator of the signalling from chiS to the promoter.
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'''pChitin:''' This 100bp region upstream of chiP is thought to be activated by the activation of chiS.
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'''PChitin:''' This 100bp region upstream of chiP is thought to be activated by the activation of chiS.
== Alert System: ==
== Alert System: ==

Latest revision as of 01:42, 27 October 2012

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Contents

Parts

Toxin- Antitoxin (TA) Modules

Our Basic Parts

TA BioBricks1-2.png


Our Composite Parts

TA BioBricks3.png

We send all this parts to the Registry!!!!

HipA7 TisB HipB MqsA istR

prm-hipA7 plac-hipB ptetR-mqsR plac-istR

Ralstonia:

Ralstoniaparts.png


pchS: It encodes a histidine kinase sensor of a two-component signal transduction system. Responds to threshold concentrations of the Quorum Sensing unusual signal 3-OH PAME.

pchR: Receptor of a two-component signal transduction system. Responds to threshold concentrations of the Quorum Sensing unusual signal 3-OH PAME. It is an atypical response regulator that has a histidine kinase as its output domain. Autophosphorilation after 3OH-PAME recognition may have en effect in phcA activation.

pchA:LysR-type transcriptional regulator that controls expression of many genes and is induced in a density manner, relying on the unusual Quorum Sensing signal 3OH-PAME.

PxpsR: Promoter region of xpsR (PxpsR) wich integrates different signals including phcA. Expression of genes regulated by XpsR may be correlated with threshold concentrations of 3 OH-PAME.

PxpsR-eYFP: Fluorescent Reporter to asses the response of PxpsR to different concentrations of 3OH-PAME.

Aliivibrio fischeri

Alis.png

The overall model can be seen in figures in the main page of Aliivibrio/Streptomyces.

chiP: Chitoporin is a specific channel that allows N-acetylglucosamine dimers to cross the outer membrane to the periplasmic space,

chiA: Chitinase is an hydrolase that cleaves the glycosidic bonds on the chitin polymer.

chiS: The histidine-kinase sensor is part of a two-component signalling system, that activates a promoter, in this case pChitin.

cbp: Chitin-binding protein is able to bind the N-acetyl glucosamine dimers in the periplasmic space. The complex CBP+(GlcNAc)2 serves as an activator of the signalling from chiS to the promoter.

PChitin: This 100bp region upstream of chiP is thought to be activated by the activation of chiS.

Alert System:

Cutandpasteparts.png