Latest revision as of 01:42, 27 October 2012

Template:Https://2012.igem.org/User:Tabima

Parts

Toxin- Antitoxin (TA) Modules

Our Basic Parts

Our Composite Parts

We send all this parts to the Registry!!!!

HipA7 TisB HipB MqsA istR

prm-hipA7 plac-hipB ptetR-mqsR plac-istR

Ralstonia:

pchS: It encodes a histidine kinase sensor of a two-component signal transduction system. Responds to threshold concentrations of the Quorum Sensing unusual signal 3-OH PAME.

pchR: Receptor of a two-component signal transduction system. Responds to threshold concentrations of the Quorum Sensing unusual signal 3-OH PAME. It is an atypical response regulator that has a histidine kinase as its output domain. Autophosphorilation after 3OH-PAME recognition may have en effect in phcA activation.

pchA:LysR-type transcriptional regulator that controls expression of many genes and is induced in a density manner, relying on the unusual Quorum Sensing signal 3OH-PAME.

PxpsR: Promoter region of xpsR (PxpsR) wich integrates different signals including phcA. Expression of genes regulated by XpsR may be correlated with threshold concentrations of 3 OH-PAME.

PxpsR-eYFP: Fluorescent Reporter to asses the response of PxpsR to different concentrations of 3OH-PAME.

Aliivibrio fischeri

The overall model can be seen in figures in the main page of Aliivibrio/Streptomyces.

chiP: Chitoporin is a specific channel that allows N-acetylglucosamine dimers to cross the outer membrane to the periplasmic space,

chiA: Chitinase is an hydrolase that cleaves the glycosidic bonds on the chitin polymer.

chiS: The histidine-kinase sensor is part of a two-component signalling system, that activates a promoter, in this case pChitin.

cbp: Chitin-binding protein is able to bind the N-acetyl glucosamine dimers in the periplasmic space. The complex CBP+(GlcNAc)2 serves as an activator of the signalling from chiS to the promoter.

PChitin: This 100bp region upstream of chiP is thought to be activated by the activation of chiS.

Alert System:

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+{{https://2012.igem.org/User:Tabima}}
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-<!-- *** End of the alert box *** -->
+== '''Parts''' ==
-{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
-!align="center"|[[Team:Colombia|Home]]
-!align="center"|[[Team:Colombia/Team|Team]]
-!align="center"|[https://igem.org/Team.cgi?year=2012&team_name=Colombia Official Team Profile]
-!align="center"|[[Team:Colombia/Project|Project]]
-!align="center"|[[Team:Colombia/Parts|Parts Submitted to the Registry]]
-!align="center"|[[Team:Colombia/Modeling|Modeling]]
-!align="center"|[[Team:Colombia/Notebook|Notebook]]
-!align="center"|[[Team:Colombia/Safety|Safety]]
-!align="center"|[[Team:Colombia/Attributions|Attributions]]
-|}
+== '''Toxin- Antitoxin (TA) Modules''' ==
-An important aspect of the iGEM competition is the use and creation of standard  biological parts. Each team will make new parts during iGEM and will place them in the [http://partsregistry.org Registry of Standard Biological Parts]. The iGEM software provides an easy way to present the parts your team has created . The "groupparts" tag will generate a table with all of the parts that your team adds to your team sandbox.  Note that if you want to document a part you need to document it on the [http://partsregistry.org Registry], not on your team wiki.
+== Our Basic Parts ==
-Remember that the goal of proper part documentation is to describe and define a part such that it can be used without a need to refer to the primary literature. The next iGEM team should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for  users who wish to know more.
+[[File:TA_BioBricks1-2.png|center|550px]]
-<groupparts>iGEM012 Colombia</groupparts>
+== Our Composite Parts ==
+[[File:TA_BioBricks3.png|center|550px]]
+='''We send all this parts to the Registry!!!! '''=
+[http://partsregistry.org/Part:BBa_K831000:Design HipA7]
+[http://partsregistry.org/Part:BBa_K831003:Design TisB]
+[http://partsregistry.org/Part:BBa_K831001:Design HipB]
+[http://partsregistry.org/Part:BBa_K831002:Design MqsA]
+[http://partsregistry.org/Part:BBa_K831004:Design ''istR'' ]
+[http://partsregistry.org/Part:BBa_K831015 prm-''hipA7'']
+[http://partsregistry.org/Part:BBa_K831009 plac-''hipB'']
+[http://partsregistry.org/Part:BBa_K831007 ptetR-''mqsR'']
+[http://partsregistry.org/Part:BBa_K831014  plac-''istR'']
+== '''Ralstonia:''' ==
+[[File:Ralstoniaparts.png|center|550px]]
+'''pchS:''' It encodes a histidine kinase sensor of a two-component signal transduction system. Responds to threshold concentrations of the Quorum Sensing unusual signal 3-OH PAME.
+'''pchR:''' Receptor of a two-component signal transduction system. Responds to threshold concentrations of the Quorum Sensing unusual signal 3-OH PAME. It is an atypical response regulator that has a histidine kinase as its output domain. Autophosphorilation after 3OH-PAME recognition may have en effect in phcA activation.
+'''pchA:'''LysR-type transcriptional regulator that controls expression of many genes and is induced in a density manner, relying on the unusual Quorum Sensing signal 3OH-PAME.
+[http://partsregistry.org/Part:BBa_K831016 '''PxpsR:'''] Promoter region of xpsR (PxpsR) wich integrates different signals including phcA. Expression of genes regulated by XpsR may be correlated with threshold concentrations of 3 OH-PAME.
+[http://partsregistry.org/Part:BBa_K831017 '''PxpsR-eYFP:'''] Fluorescent Reporter to asses the response of PxpsR to different concentrations of 3OH-PAME.
+== ''Aliivibrio fischeri'' ==
+[[File:alis.png|center|500px|]]
+The overall model can be seen in figures in [https://2012.igem.org/Team:Colombia/Project/Experiments/Aliivibrio_and_Streptomyces the main page of Aliivibrio/Streptomyces.]
+'''chiP:''' [http://www.ncbi.nlm.nih.gov/gene/3279194 Chitoporin] is a specific channel that allows N-acetylglucosamine dimers to cross the outer membrane to the periplasmic space,
+'''chiA:''' [http://www.ncbi.nlm.nih.gov/gene/3277358 Chitinase] is an hydrolase that cleaves the glycosidic bonds on the chitin polymer.
+'''chiS:''' [http://www.ncbi.nlm.nih.gov/gene/3279287 The histidine-kinase sensor] is part of a two-component signalling system, that activates a promoter, in this case pChitin.
+'''cbp:''' [http://www.ncbi.nlm.nih.gov/gene/3279341 Chitin-binding protein] is able to bind the N-acetyl glucosamine dimers in the periplasmic space. The complex CBP+(GlcNAc)2 serves as an activator of the signalling from chiS to the promoter.
+'''PChitin:''' This 100bp region upstream of chiP is thought to be activated by the activation of chiS.
+== Alert System: ==
+[[File:Cutandpasteparts.png|center|600px]]
+</div>

Team:Colombia/Parts

From 2012.igem.org