"

From 2012.igem.org

Contents 1 Bacterial DNA extraction protocol 2 Miniprep 3 Electroporation 4 PCR - Pfu DNA polimerase 5 PCR - Invitrogen (R) Taq polimerase

Bacterial DNA extraction protocol

Miniprep

Electroporation

Electro-competent cells of E. coli DH5α are electroporated at 1.25 mV, immediately we add SOC medium to the bacteria and we incubated on a shaker at 37°C for one hour, then, we culture cells in a solid medium containing the antibiotic of selection.

PCR - Pfu DNA polimerase

Reactives	Amount (μL)
H ~2~ O	10
Buffer	4
MgSO_4	1

PCR - Invitrogen (R) Taq polimerase