Team:Colombia/Notebook/Protocols

From 2012.igem.org

(Difference between revisions)
(Created page with "=='''Bacterial DNA extraction protocol'''== =='''Miniprep'''== =='''Electroporation'''== =='''PCR - Pfu DNA polimerase'''== =='''PCR - Invitrogen (R) Taq polimerase'''==")
(Electroporation)
Line 4: Line 4:
=='''Electroporation'''==
=='''Electroporation'''==
 +
 +
Electro-competent cells of E. coli DH5α are electroporated at 1.25 mV, immediately we add SOC medium to the bacteria and we incubated on a shaker at 37°C for one hour, then, we culture cells in a solid medium containing the antibiotic of selection.
=='''PCR - Pfu DNA polimerase'''==
=='''PCR - Pfu DNA polimerase'''==
=='''PCR - Invitrogen (R) Taq polimerase'''==
=='''PCR - Invitrogen (R) Taq polimerase'''==

Revision as of 21:32, 13 July 2012

Contents

Bacterial DNA extraction protocol

Miniprep

Electroporation

Electro-competent cells of E. coli DH5α are electroporated at 1.25 mV, immediately we add SOC medium to the bacteria and we incubated on a shaker at 37°C for one hour, then, we culture cells in a solid medium containing the antibiotic of selection.

PCR - Pfu DNA polimerase

PCR - Invitrogen (R) Taq polimerase