Team:Colombia/Notebook/Protocols
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Gutiloluis (Talk | contribs) (Created page with "=='''Bacterial DNA extraction protocol'''== =='''Miniprep'''== =='''Electroporation'''== =='''PCR - Pfu DNA polimerase'''== =='''PCR - Invitrogen (R) Taq polimerase'''==") |
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=='''Electroporation'''== | =='''Electroporation'''== | ||
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+ | Electro-competent cells of E. coli DH5α are electroporated at 1.25 mV, immediately we add SOC medium to the bacteria and we incubated on a shaker at 37°C for one hour, then, we culture cells in a solid medium containing the antibiotic of selection. | ||
=='''PCR - Pfu DNA polimerase'''== | =='''PCR - Pfu DNA polimerase'''== | ||
=='''PCR - Invitrogen (R) Taq polimerase'''== | =='''PCR - Invitrogen (R) Taq polimerase'''== |
Revision as of 21:32, 13 July 2012
Contents |
Bacterial DNA extraction protocol
Miniprep
Electroporation
Electro-competent cells of E. coli DH5α are electroporated at 1.25 mV, immediately we add SOC medium to the bacteria and we incubated on a shaker at 37°C for one hour, then, we culture cells in a solid medium containing the antibiotic of selection.