"
Team:Colombia/Notebook/Journal
From 2012.igem.org
(→Chitinase) |
(→Chitinase) |
||
| Line 1: | Line 1: | ||
{{Http://2012.igem.org/User:Tabima}} | {{Http://2012.igem.org/User:Tabima}} | ||
| + | |||
== Chitinase == | == Chitinase == | ||
| Line 10: | Line 11: | ||
-'' Trichoderma spp.'' | -'' Trichoderma spp.'' | ||
| + | |||
| + | |||
For each one we obtained accesion numbers: | For each one we obtained accesion numbers: | ||
| Line 18: | Line 21: | ||
-'' Trichoderma spp.'' : [http://www.ncbi.nlm.nih.gov/nucest/BM077089.2 BM077089.2] | -'' Trichoderma spp.'' : [http://www.ncbi.nlm.nih.gov/nucest/BM077089.2 BM077089.2] | ||
| + | |||
| + | |||
Each one was tested using codon usage for bacteria (transl_table=11, [www.ncbi.nlm.nih.gov/Taxonomy/Utils/wprintgc.cgi NCBI link]) to determine if the chitinase could be used. '''All of the sequences were suitable for use'''. | Each one was tested using codon usage for bacteria (transl_table=11, [www.ncbi.nlm.nih.gov/Taxonomy/Utils/wprintgc.cgi NCBI link]) to determine if the chitinase could be used. '''All of the sequences were suitable for use'''. | ||
| + | |||
| + | |||
We selected ''Colletotrichum spp.'' and ''Trichoderma spp.'' sequences to design primers. | We selected ''Colletotrichum spp.'' and ''Trichoderma spp.'' sequences to design primers. | ||
| + | |||
| + | |||
'''Primers''' | '''Primers''' | ||
Revision as of 20:53, 26 June 2012
Chitinase
To determine which chitinase is going to be used, we screened chitinase from 3 different species since the chitinase of different Vibrio spp. was not suitable or the strain was found (Alivibrio fischeri ES114, Alivibrio fischeri M11):
- Arabidopsis thaliana
- Colletotrichum spp.
- Trichoderma spp.
For each one we obtained accesion numbers:
- Arabidopsis thaliana: AY099810.2
- Colletotrichum spp. : GW342409.1
- Trichoderma spp. : BM077089.2
Each one was tested using codon usage for bacteria (transl_table=11, [www.ncbi.nlm.nih.gov/Taxonomy/Utils/wprintgc.cgi NCBI link]) to determine if the chitinase could be used. All of the sequences were suitable for use.
We selected Colletotrichum spp. and Trichoderma spp. sequences to design primers.
Primers
- Colletotrichum spp. : - Trichoderma spp. :
