Team:Colombia

From 2012.igem.org

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(Replaced content with "= '''Team Colombia @ 2012 iGEM''' = {{http://2012.igem.org/User:Tabima}} center")
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= '''Team Colombia @ 2012 iGEM''' =
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{{http://2012.igem.org/User:Tabima}}
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    display: block;
 +
    clear: both;
 +
}
 +
body{
 +
    font-size: 76%;
 +
    background: #ffffff;
 +
}
 +
#hokkaidou-container{
 +
    width: 980px;
 +
    margin: 0 auto;
 +
    padding: 0;
 +
}
 +
#hokkaidou-header{
 +
    background: #ffffff url(http://2011.igem.org/wiki/images/2/2b/Hokkaidou_header.png) repeat-x;
 +
    margin-bottom: 10px;
 +
}
 +
#hokkaidou-footer{
 +
    margin-top: 30px;
 +
    padding-top: 15px;
 +
    border-top: 1px solid #e4e4e4;
 +
    text-align: center;
 +
}
 +
#hokkaidou-footer #hokkaidou-sponsors ul li{
 +
    display: inline-block;
 +
    margin: 10px;
 +
    list-style: none;
 +
}
 +
#hokkaidou-footer #hokkaidou-sponsors ul:after {content: "";}
 +
</style>
 +
</p><p><style>
 +
#hokkaidou-header{
 +
    position: relative;
 +
    height: 117px;
 +
}
 +
#hokkaidou-header #hokkaidou-logo h1 a{
 +
    display: block;
 +
    position: absolute;
 +
    top: 10px; left: 20px;
 +
    width: 400px;
 +
    height: 75px;
 +
    background: url(http://2011.igem.org/wiki/images/e/e5/Hokkaidou_logo.png) no-repeat;
 +
    border: none;
 +
    text-indent: -10000px;
 +
    outline: none;
 +
}
 +
#hokkaidou-header #hokkaidou-logo h1 a:hover {
 +
    background-position: 0 -76px;
 +
}
 +
#hokkaidou-header #hokkaidou-logo h1 a:active {
 +
    background-position: 0 -152px;
 +
}
 +
#hokkaidou-header #hokkaidou-logo p{
 +
    display: none !important;
 +
}
 +
</style>
 +
</p><p><style>
 +
#hokkaidou-header #hokkaidou-navigation ul{
 +
    position: absolute;
 +
    top: 10px; right: 0;
 +
}
 +
#hokkaidou-header #hokkaidou-navigation ul li{
 +
    float: left;
 +
    list-style: none;
 +
    margin: 0;
 +
    text-align: center;
 +
    width: 60px;
 +
}
 +
#hokkaidou-header #hokkaidou-navigation ul li a,
 +
#hokkaidou-header #hokkaidou-navigation ul li strong.selflink{
 +
    display: inline-block;
 +
    width: 30px; height: 30px;
 +
    background: url(http://2011.igem.org/wiki/images/4/4b/Hokkaidou_menu.png) no-repeat;
 +
    border: none;
 +
    outline: none;
 +
    text-indent: -10000px;
 +
}
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-home a{ background-position: 0 0; }
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-home a:hover { background-position: -30px 0; }
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-home a:active,
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-home strong.selflink{ background-position: -60px 0; }
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-project a{ background-position: 0 -30px; }
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-project a:hover { background-position: -30px -30px; }
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-project a:active,
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-project strong.selflink{ background-position: -60px -30px; }
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-protocol a{ background-position: 0 -60px; }
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-protocol a:hover { background-position: -30px -60px; }
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-protocol a:active,
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-protocol strong.selflink{ background-position: -60px -60px; }
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-parts a{ background-position: 0 -90px; }
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-parts a:hover { background-position: -30px -90px; }
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-parts a:active,
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-parts strong.selflink{ background-position: -60px -90px; }
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-notebook a{ background-position: 0 -120px; }
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-notebook a:hover { background-position: -30px -120px; }
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-notebook a:active,
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-notebook strong.selflink{ background-position: -60px -120px; }
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-safety a{ background-position: 0 -150px; }
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-safety a:hover { background-position: -30px -150px; }
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-safety a:active,
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-safety strong.selflink{ background-position: -60px -150px; }
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-humanpractice a{ background-position: 0 -180px; }
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-humanpractice a:hover { background-position: -30px -180px; }
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-humanpractice a:active,
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-humanpractice strong.selflink{ background-position: -60px -180px; }
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-team a{ background-position: 0 -210px; }
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-team a:hover { background-position: -30px -210px; }
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-team a:active,
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-team strong.selflink{ background-position: -60px -210px; }
 +
 
 +
/* i couldn't be bothered to add text on picture... lol */
 +
#hokkaidou-header #hokkaidou-navigation ul li:after {
 +
    display: inline-block;
 +
    font-family: 'Josefin Sans', sans-serif;
 +
    font-size: 12px;
 +
    margin-top: 5px;
 +
    width: 100%;
 +
    line-height: 14px;
 +
}
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-home:after {content: "Home";}
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-project:after {content: "Project";}
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-protocol:after {content: "Protocol";}
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-parts:after {content: "Parts";}
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-notebook:after {content: "Notebook";}
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-safety:after {content: "Safety";}
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-humanpractice:after {content: "Human Practice";}
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-team:after {content: "Team";}
 +
</style>
 +
</p><p><style>
 +
#hokkaidou-content{zoom: 1;}
 +
#hokkaidou-content:after { content: ""; }
 +
#hokkaidou-content #hokkaidou-left-content{
 +
    float: left;
 +
    width: 280px;
 +
    margin-right: 20px;
 +
}
 +
#hokkaidou-content #hokkaidou-left-content ul{
 +
    margin: 0;
 +
    padding: 0 1em;
 +
}
 +
#hokkaidou-content #hokkaidou-left-content ul li{
 +
    list-style: none;
 +
    margin: 0 0 1em 0;
 +
}
 +
#hokkaidou-content #hokkaidou-left-content ul li .hokkaidou-description{
 +
    font-size: small;
 +
    color: #8a8a8a;
 +
}
 +
#hokkaidou-content #hokkaidou-right-content{
 +
    float: right;
 +
    width: 680px;
 +
}
 +
</style>
 +
</p><p><style>
 +
#hokkaidou-index-menu{
 +
    margin: 0 0 30px 0 !important;
 +
    padding: 0 !important;
 +
    border: 1px solid #ababab;
 +
    -webkit-border-top-right-radius: 12px;
 +
    -moz-border-radius-topright: 12px;
 +
    border-top-right-radius: 12px;
 +
    -webkit-border-bottom-right-radius: 12px;
 +
    -moz-border-radius-bottomright: 12px;
 +
    border-bottom-right-radius: 12px;
 +
}
 +
#hokkaidou-index-menu li{
 +
    list-style: none;
 +
    margin: 0 !important;
 +
    padding: 0 !important;
 +
    background: -moz-radial-gradient(center 80px 45deg, circle cover, hsl(0, 0%, 100%) 0%, hsl(240, 5%, 70%) 100%);
 +
    overflow: hidden;
 +
    text-shadow: 0 1px 1px hsl(240, 10%, 80%);
 +
}
 +
#hokkaidou-index-menu li:first-child{
 +
    -webkit-border-top-right-radius: 12px;
 +
    -moz-border-radius-topright: 12px;
 +
    border-top-right-radius: 12px;
 +
}
 +
#hokkaidou-index-menu li:last-child{
 +
    -webkit-border-bottom-right-radius: 12px;
 +
    -moz-border-radius-bottomright: 12px;
 +
    border-bottom-right-radius: 12px;
 +
}
 +
 
 +
#hokkaidou-index-menu li a{
 +
    display: block;
 +
    padding: 10px 20px;
 +
    border-bottom: none;
 +
    color: #3b3b3b;
 +
    transition: background 0.5s ease-in-out;
 +
    -moz-transition-property: background;
 +
    -moz-transition-duration: 700ms;
 +
    background: #e4e4e4;
 +
    border-bottom: 1px solid #dedede;
 +
    border-top: 1px solid #f7f7f7;
 +
}
 +
#hokkaidou-index-menu li a{
 +
}
 +
#hokkaidou-index-menu li a:hover{
 +
    background: transparent none;
 +
}
 +
#hokkaidou-index-menu li a .hokkaidou-description{
 +
    display: block;
 +
    margin-left: 40px;
 +
}
 +
#hokkaidou-index-menu li a:before{
 +
    display: block;
 +
    float: left;
 +
    content: "";
 +
    width: 30px; height: 30px;
 +
    margin: 5px 10px 0 0;
 +
    background: url(http://2011.igem.org/wiki/images/4/4b/Hokkaidou_menu.png) no-repeat;
 +
}             
 +
#hokkaidou-index-menu li#hokkaidou-index-project a:before{ background-position: 0 -30px; }
 +
#hokkaidou-index-menu li#hokkaidou-index-protocol a:before{ background-position: 0 -60px; }
 +
#hokkaidou-index-menu li#hokkaidou-index-parts a:before{ background-position: 0 -90px; }
 +
#hokkaidou-index-menu li#hokkaidou-index-notebook a:before{ background-position: 0 -120px; }
 +
#hokkaidou-index-menu li#hokkaidou-index-safety a:before{ background-position: 0 -150px; }
 +
#hokkaidou-index-menu li#hokkaidou-index-humanpractice a:before{ background-position: 0 -180px; }
 +
#hokkaidou-index-menu li#hokkaidou-index-team a:before{ background-position: 0 -210px; }
 +
 
 +
#hokkaidou-index-menu li#hokkaidou-index-project a:hover:before{ background-position: -30px -30px; }
 +
#hokkaidou-index-menu li#hokkaidou-index-protocol a:hover:before{ background-position: -30px -60px; }
 +
#hokkaidou-index-menu li#hokkaidou-index-parts a:hover:before{ background-position: -30px -90px; }
 +
#hokkaidou-index-menu li#hokkaidou-index-notebook a:hover:before{ background-position: -30px -120px; }
 +
#hokkaidou-index-menu li#hokkaidou-index-safety a:hover:before{ background-position: -30px -150px; }
 +
#hokkaidou-index-menu li#hokkaidou-index-humanpractice a:hover:before{ background-position: -30px -180px; }
 +
#hokkaidou-index-menu li#hokkaidou-index-team a:hover:before{ background-position: -30px -210px; }
 +
 
 +
#hokkaidou-official-team-logo{
 +
    text-align: center;
 +
}
 +
#hokkaidou-official-team-logo img{
 +
    display: block;
 +
    width: 240px;
 +
    height: 240px;
 +
    border: 1px solid #e4e4e4;
 +
    margin: 0 auto;
 +
}
 +
#hokkaidou-official-team-logo .hokkaidou-description{
 +
    font-size: small;
 +
    color: #ababab;
 +
}
 +
#hokkaidou-links ul{
 +
    margin: 20px 0 !important;
 +
    padding: 0 !important;
 +
}
 +
#hokkaidou-links ul {zoom: 1;}
 +
#hokkaidou-links ul:after {content: ""; }
 +
#hokkaidou-links ul li{
 +
    float: left;
 +
    display: block !important;
 +
    margin: 0 5px !important;
 +
    padding: 0 !important;
 +
}
 +
#hokkaidou-links ul li:first-child{
 +
    margin-left: 0 !important;
 +
}
 +
#hokkaidou-links ul li a{
 +
    display: block;
 +
    width: 32px;
 +
    height: 32px;
 +
    background: url(http://2011.igem.org/wiki/images/8/8b/IGEM_HokkaidoU_Index_Links.png) no-repeat;
 +
    text-indent: -10000px;
 +
}
 +
#hokkaidou-links ul li#hokkaidou-links-email a{ background-position: 0 0; }
 +
#hokkaidou-links ul li#hokkaidou-links-facebook a{ background-position: -32px 0; }
 +
#hokkaidou-links ul li#hokkaidou-links-twitter a{ background-position: -64px 0; }
 +
#hokkaidou-links ul li#hokkaidou-links-wordpress a{ background-position: -96px 0; }
 +
#hokkaidou-links ul li#hokkaidou-links-youtube a{ background-position: -128px 0; }
 +
#hokkaidou-links ul li#hokkaidou-links-email{
 +
    display: block !important;
 +
    width: 32px !important;
 +
    height: 32px !important;
 +
    margin: 0 5px 0 0 !important;
 +
    padding: 0 !important;
 +
}
 +
#hokkaidou-links ul li#hokkaidou-links-email a{
 +
    display: block !important;
 +
    width: 100% !important;
 +
    height: 100% !important;
 +
    margin: 0 !important;
 +
    padding: 0 !important;
 +
    background: url(http://2011.igem.org/wiki/images/8/8b/IGEM_HokkaidoU_Index_Links.png) no-repeat !important;
 +
    text-indent: -10000px !important;
 +
}
 +
</style>
 +
 
 +
<script type="text/javascript">
 +
jQuery.cookie=function(d,f,b){function e(j,i){if(j){if(i){return true}}return false}if(e(arguments.length>1,String(f)!=="[object Object]")){b=jQuery.extend({},b);if(f===null||f===undefined){b.expires=-1}if(typeof b.expires==="number"){var h=b.expires,c=b.expires=new Date();c.setDate(c.getDate()+h)}f=String(f);return(document.cookie=[encodeURIComponent(d),"=",b.raw?f:encodeURIComponent(f),b.expires?"; expires="+b.expires.toUTCString():"",b.path?"; path="+b.path:"",b.domain?"; domain="+b.domain:"",b.secure?"; secure":""].join(""))}b=f||{};var a,g=b.raw?function(i){return i}:decodeURIComponent;return(a=new RegExp("(?:^|; )"+encodeURIComponent(d)+"=([^;]*)").exec(document.cookie))?g(a[1]):null};
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</script>
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<script type="text/javascript">
 +
$('.right-menu, .left-menu').fadeTo(0, 0);
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$('.right-menu, .left-menu').bind('mouseover', function(){
 +
$(this).fadeTo('slow', 1);
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});
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</script>
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<script type="text/javascript">
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</script>
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<script type="text/javascript">
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$(function(){
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    var open = $.cookie('igem_hokkaidou_toc');
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    $.cookie('igem_hokkaidou_toc', open);
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    var $toc = $('#toc');
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    var $tab = $('<div>').addClass('toc-tab');
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    var $close = $('<div>').addClass('close');
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    var $wrapper = $('<div>').addClass('toc-wrapper');
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    $toc.before($tab);
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    $toc.wrap($wrapper);
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    <div id="hokkaidou-header">
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            <h1> <span class="mw-headline" id="HokkaidoU_Japan"><a href="http://2011.igem.org/Team:HokkaidoU_Japan">HokkaidoU Japan</a></span></h1>
 +
            <p class="description">iGEM 2011 Team of Hokkaido University</p>
 +
        </div>
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        <div id="hokkaidou-navigation"><ul>
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            <li id="hokkaidou-menu-home"><strong class="selflink">Home</strong></li>
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            <li id="hokkaidou-menu-team"><a href="/Team:HokkaidoU_Japan/Team" title="Team:HokkaidoU Japan/Team">Team</a></li>
 +
            <li id="hokkaidou-menu-project"><a href="/Team:HokkaidoU_Japan/Project" title="Team:HokkaidoU Japan/Project">Project</a></li>
 +
            <li id="hokkaidou-menu-parts"><a href="/Team:HokkaidoU_Japan/Parts" title="Team:HokkaidoU Japan/Parts">Parts</a></li>
 +
            <li id="hokkaidou-menu-protocol"><a href="/Team:HokkaidoU_Japan/Protocols" title="Team:HokkaidoU Japan/Protocols">Protocols</a></li>
 +
            <li id="hokkaidou-menu-notebook"><a href="/Team:HokkaidoU_Japan/Notebook" title="Team:HokkaidoU Japan/Notebook">Notebook</a></li>
 +
            <li id="hokkaidou-menu-safety"><a href="/Team:HokkaidoU_Japan/Safety" title="Team:HokkaidoU Japan/Safety">Safety</a></li>
 +
            <li id="hokkaidou-menu-humanpractice"><a href="/Team:HokkaidoU_Japan/HumanPractice" title="Team:HokkaidoU Japan/HumanPractice">Human Practice</a></li>
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    </div>
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    <div id="hokkaidou-content">
 +
    <table id="toc" class="toc"><tr><td><div id="toctitle"><h2>Contents</h2></div>
 +
<ul>
 +
<li class="toclevel-1"><a href="#HokkaidoU_Japan"><span class="tocnumber">1</span> <span class="toctext">HokkaidoU Japan</span></a>
 +
<ul>
 +
<li class="toclevel-2 tocsection-1"><a href="#Acknowledgements"><span class="tocnumber">1.1</span> <span class="toctext">Acknowledgements</span></a>
 +
<ul>
 +
<li class="toclevel-3 tocsection-2"><a href="#Laboratory_and_Personnel"><span class="tocnumber">1.1.1</span> <span class="toctext">Laboratory and Personnel</span></a></li>
 +
<li class="toclevel-3 tocsection-3"><a href="#Academic_Institutions"><span class="tocnumber">1.1.2</span> <span class="toctext">Academic Institutions</span></a></li>
 +
<li class="toclevel-3 tocsection-4"><a href="#Companies"><span class="tocnumber">1.1.3</span> <span class="toctext">Companies</span></a></li>
 +
</ul>
 +
</li>
 +
</ul>
 +
</li>
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</ul>
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        <li id="hokkaidou-index-team"><a href="http://2011.igem.org/Team:HokkaidoU_Japan/Team">Team
 +
            <span class="hokkaidou-description">
 +
                The HokkaidoU_Japan is formed by 16 students and 3 instructors
 +
            </span>
 +
        </a>
 +
        </li>
 +
        <li id="hokkaidou-index-project"><a href="http://2011.igem.org/Team:HokkaidoU_Japan/Project">Project
 +
            <span class="hokkaidou-description">
 +
                We tried to further develop E. coli-based protein injection system which we started working on during iGEM 2010
 +
            </span>
 +
        </a>
 +
        </li>
 +
        <li id="hokkaidou-index-parts"><a href="http://2011.igem.org/Team:HokkaidoU_Japan/Parts">Parts
 +
            <span class="hokkaidou-description">
 +
                Ready-to-inject backbone and Bsa I cloning system
 +
            </span>
 +
        </a>
 +
        </li>
 +
        <li id="hokkaidou-index-protocol"><a href="http://2011.igem.org/Team:HokkaidoU_Japan/Protocols">Protocol
 +
            <span class="hokkaidou-description">
 +
                General protocols, Primers and methods of infection assay
 +
            </span>
 +
        </a>
 +
        </li>
 +
        <li id="hokkaidou-index-notebook"><a href="http://2011.igem.org/Team:HokkaidoU_Japan/Notebook">Notebook
 +
            <span class="hokkaidou-description">
 +
                The record of our many failures and a bit of achievement
 +
            </span>
 +
        </a>
 +
        </li>
 +
        <li id="hokkaidou-index-safety"><a href="http://2011.igem.org/Team:HokkaidoU_Japan/Safety">Safety
 +
            <span class="hokkaidou-description">
 +
                Addressing safety concerns
 +
            </span>
 +
        </a>
 +
        </li>
 +
        <li id="hokkaidou-index-humanpractice"><a href="http://2011.igem.org/Team:HokkaidoU_Japan/HumanPractice">Human Practice
 +
            <span class="hokkaidou-description">
 +
                Science art gallery "BIO ART"
 +
            </span>
 +
        </a>
 +
        </li>
 +
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        <span class="hokkaidou-description">iGEM HokkaidoU_Japan Official Team Logo</span>
 +
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            <li id="hokkaidou-links-email"><a href="mailto:igemhokkaidou@gmail.com">Email</a></li>
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            <li id="hokkaidou-links-facebook"><a href="http://www.facebook.com/iGEMHokkaidoU">Facebook</a></li>
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            <li id="hokkaidou-links-twitter"><a href="http://twitter.com/iGEM_HokkaidoU">Twitter</a></li>
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            <li id="hokkaidou-links-youtube"><a href="http://www.youtube.com/user/iGEMHokkaidoU">YouTube</a></li>
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        </ul>
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 +
            <ul style="left: -4200px; width: 6300px;" class="lof-main-wapper">
 +
                <li>
 +
                    <img src="http://2011.igem.org/wiki/images/6/64/IGEM_HokkaidoU_Index_Slideshow_7.jpg">         
 +
                    <div class="lof-main-item-desc">
 +
                        <h2>HokkaidoU_Japan 2011</h2>
 +
                    </div>
 +
                </li>
 +
                <li>
 +
                    <img src="http://2011.igem.org/wiki/images/8/8a/IGEM_HokkaidoU_Index_Slideshow_1.jpg">         
 +
                    <div class="lof-main-item-desc">
 +
                        <h2>Welcome to the BIOxART Gallery!!</h2>
 +
                    </div>
 +
                </li>
 +
                <li>
 +
                    <img src="http://2011.igem.org/wiki/images/b/b5/IGEM_HokkaidoU_Index_Slideshow_2.jpg">         
 +
                    <div class="lof-main-item-desc">
 +
                        <h2>Be careful not to contaminate...</h2>
 +
                    </div>
 +
                </li>
 +
                <li>
 +
                    <img src="http://2011.igem.org/wiki/images/0/01/IGEM_HokkaidoU_Index_Slideshow_3.jpg">           
 +
                    <div class="lof-main-item-desc">
 +
                        <h2>Meeting, meeting and MEETING!</h2>
 +
                    </div>
 +
                </li>
 +
                <li>
 +
                    <img src="http://2011.igem.org/wiki/images/d/d1/IGEM_HokkaidoU_Index_Slideshow_4.jpg">           
 +
                    <div class="lof-main-item-desc">
 +
                        <h2>Dr. <i>E. coli</i> get ready for the job</h2>
 +
                    </div>
 +
                </li>
 +
                <li>
 +
                    <img src="http://2011.igem.org/wiki/images/0/06/IGEM_HokkaidoU_Index_Slideshow_5.jpg">           
 +
                    <div class="lof-main-item-desc">
 +
                        <h2>BIOxART, please feel free to look around</h2>
 +
                    </div>
 +
                </li>
 +
                <li>
 +
                    <img src="http://2011.igem.org/wiki/images/7/74/IGEM_HokkaidoU_Index_Slideshow_6.jpg">           
 +
                    <div class="lof-main-item-desc">
 +
                        <h2>Experiment, experiment and EXPERIMENT!</h2>
 +
                    </div>
 +
                </li>
 +
            </ul>   
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                    <li class="" style="height: 32px; width: 80px;"><img src="http://2011.igem.org/wiki/images/8/81/IGEM_HokkaidoU_Index_Slideshow_Thumb_7.jpg"></li>
 +
                    <li class="" style="height: 32px; width: 80px;"><img src="http://2011.igem.org/wiki/images/f/f3/IGEM_HokkaidoU_Index_Slideshow_Thumb_1.jpg"></li>
 +
                    <li class="" style="height: 32px; width: 80px;"><img src="http://2011.igem.org/wiki/images/a/aa/IGEM_HokkaidoU_Index_Slideshow_Thumb_2.jpg"></li>
 +
                    <li class="" style="height: 32px; width: 80px;"><img src="http://2011.igem.org/wiki/images/f/fb/IGEM_HokkaidoU_Index_Slideshow_Thumb_3.jpg"></li>
 +
                    <li class="" style="height: 32px; width: 80px;"><img src="http://2011.igem.org/wiki/images/e/e2/IGEM_HokkaidoU_Index_Slideshow_Thumb_4.jpg"></li>
 +
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 +
                    <li class="" style="height: 32px; width: 80px;"><img src="http://2011.igem.org/wiki/images/2/21/IGEM_HokkaidoU_Index_Slideshow_Thumb_6.jpg"></li>
 +
                </ul>
 +
            </div>
 +
        </div>
 +
    </div>
 +
    <h2>Abstract</h2>
 +
    <p>We further developed "Dr. E. coli": our project of iGEM 2010. Last year, we showed that Type 3 Secretion System (T3SS) works in E. coli by injecting GFP into RK13 cells. T3SS is a syringe like organelle found in bacterium such as Salmonella which uses it to inject virulence effector proteins into a target eukaryotic cell. We think this system can be applied to direct reprogramming of somatic cells among many other things.</p>
 +
    <p>We tested T3SS performance and tried to make it more convenient. For this purpose we designed a plasmid backbone which can instantly produce ready-to-inject fusion proteins from ordinary biobrick part. Using it, we tried to further characterize this system by injecting a library of protein domains.</p>
 +
    <p>In Science Gallery we exhibited awesome photographs related with biotechnology in public. We tried to catch the pedestrians’ interest in current synthetic biology and explore their thoughts.</p>
 +
 
 +
    <h2>BioArt</h2>
 +
    <iframe id="hokkaidou-bioart-youtube" width="680" height="360" src="http://www.youtube.com/embed/nOP0YIUSj-s" frameborder="0" allowfullscreen></iframe>
 +
    <style type="text/css">
 +
        iframe#hokkaidou-bioart-youtube{ margin-bottom: 30px; }
 +
    </style>
 +
 
 +
    <p>We held the new type science art gallery "Bio Art" at the center of Sapporo city.</p>
 +
    <p>This gallery is not only for our team.You can also enjoy Bio Art on this Web page.
 +
    You can watch more detail in <a href="http://2011.igem.org/Team:HokkaidoU_Japan/HumanPractice">Human Practice</a> page.</p>
 +
 +
 
 +
</p>
 +
<h2> <span class="mw-headline" id="Acknowledgements">Acknowledgements</span></h2>
 +
<h3> <span class="mw-headline" id="Laboratory_and_Personnel">Laboratory and Personnel</span></h3>
 +
<ul><li> Environmental Molecular Biology, Graduate School of Environmental Earth Science, Hokkaido University
 +
</li></ul>
 +
<dl><dd><dl><dd> Ken-ichi Yamazaki and Members of Yamazaki Lab
 +
</dd></dl>
 +
</dd></dl>
 +
<ul><li> Molecular Biology, Institute for Genetic Medicine, Hokkaido University
 +
</li></ul>
 +
<dl><dd><dl><dd> Hisatoshi Shida
 +
</dd></dl>
 +
</dd></dl>
 +
<ul><li> Nikon Imaging Center
 +
</li></ul>
 +
<dl><dd><dl><dd> Takeharu Nagai, Kenta Saito, Masahiro Nakano, Kentarou Kobayashi, Hiroshi Nishino
 +
</dd></dl>
 +
</dd></dl>
 +
<ul><li> Laboratory of Molecular and Cellular Biophysics, Hokkaido University
 +
</li></ul>
 +
<dl><dd><dl><dd> Tomomi Nemoto, Terumasa Hibi, Ryosuke Kawakami
 +
</dd></dl>
 +
</dd></dl>
 +
<ul><li> Muroran Marine Station Field Science Center for Northern Biosphere, Hokkaido University
 +
</li></ul>
 +
<dl><dd><dl><dd> Taizo Motomura
 +
</dd></dl>
 +
</dd></dl>
 +
<ul><li> Laboratory of X-ray structural biology, Department of Advanced Transdisciplinary Science, faculty of Advanced Life Science
 +
</li></ul>
 +
<dl><dd><dl><dd>Isao Tanaka
 +
</dd></dl>
 +
</dd></dl>
 +
<ul><li> Research Institute for Electronic Science, Hokkaido University
 +
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Revision as of 08:02, 15 July 2012

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> Team:HokkaidoU Japan - 2011.igem.org

 

Team:HokkaidoU Japan

From 2011.igem.org

Contents

  • HokkaidoU_Japan 2011

  • Welcome to the BIOxART Gallery!!

  • Be careful not to contaminate...

  • Meeting, meeting and MEETING!

  • Dr. E. coli get ready for the job

  • BIOxART, please feel free to look around

  • Experiment, experiment and EXPERIMENT!

Abstract

We further developed "Dr. E. coli": our project of iGEM 2010. Last year, we showed that Type 3 Secretion System (T3SS) works in E. coli by injecting GFP into RK13 cells. T3SS is a syringe like organelle found in bacterium such as Salmonella which uses it to inject virulence effector proteins into a target eukaryotic cell. We think this system can be applied to direct reprogramming of somatic cells among many other things.

We tested T3SS performance and tried to make it more convenient. For this purpose we designed a plasmid backbone which can instantly produce ready-to-inject fusion proteins from ordinary biobrick part. Using it, we tried to further characterize this system by injecting a library of protein domains.

In Science Gallery we exhibited awesome photographs related with biotechnology in public. We tried to catch the pedestrians’ interest in current synthetic biology and explore their thoughts.

BioArt

We held the new type science art gallery "Bio Art" at the center of Sapporo city.

This gallery is not only for our team.You can also enjoy Bio Art on this Web page. You can watch more detail in Human Practice page.

Acknowledgements

Laboratory and Personnel

  • Environmental Molecular Biology, Graduate School of Environmental Earth Science, Hokkaido University
Ken-ichi Yamazaki and Members of Yamazaki Lab
  • Molecular Biology, Institute for Genetic Medicine, Hokkaido University
Hisatoshi Shida
  • Nikon Imaging Center
Takeharu Nagai, Kenta Saito, Masahiro Nakano, Kentarou Kobayashi, Hiroshi Nishino
  • Laboratory of Molecular and Cellular Biophysics, Hokkaido University
Tomomi Nemoto, Terumasa Hibi, Ryosuke Kawakami
  • Muroran Marine Station Field Science Center for Northern Biosphere, Hokkaido University
Taizo Motomura
  • Laboratory of X-ray structural biology, Department of Advanced Transdisciplinary Science, faculty of Advanced Life Science
Isao Tanaka
  • Research Institute for Electronic Science, Hokkaido University

Academic Institutions

  • Salmonella Genetic Stock Center/University of Calgary
  • Graduate School of Environmental Earth Science
  • Department of biological science/School of Sciense
  • Institute for Genetic Medicine

Companies

  • Primary Cell Co., Ltd.
  • ECONiXE Co., Ltd.
  • Hokuto Kohki Engineering Co,. Ltd.
  • COSMO BIO Co., Ltd.
  • AMINO UP CHEMICAL Co., Ltd.
  • Mendel Workshop Co., Ltd.