Team:Colombia

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(Team Colombia @ 2012 iGEM)
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<div class="editsection" style="float:right;margin-left:5px;">[<a href="/wiki/index.php?title=Lead&amp;action=edit&amp;section=1" title="Lead">edit</a>]</div><a name="Lead_Detection"></a><h1><a href="/wiki/index.php/%5CLead_Detection" title="\Lead Detection"> Lead Detection</a></h1>
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<p>According to the WHO, 40% of the planet does not have access to clean water. Lead is a serious contaminant around the world. It can cause neurological disorders, anemia, and death. In the United States alone, 3.5 million people have high levels of lead in their blood, so the problem is prevalent in both the developed and developing world. Children under the age of 12 are most at risk for lead poisoning. For more on lead poisoning, click <a href="http://www.aquasanastore.com/water-you_c04.html" class='external text' title="http://www.aquasanastore.com/water-you c04.html" rel="nofollow">here</a>.
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<div class="editsection" style="float:right;margin-left:5px;">[<a href="/wiki/index.php?title=Lead&amp;action=edit&amp;section=2" title="Lead">edit</a>]</div><a name="Design"></a><h1><a href="/wiki/index.php/%5CDesign" title="\Design"> Design</a></h1>
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<p>Having found a Lead Binding Protein and its corresponding Lead Promoter in the literature, we began to generate a number of ideas for our lead detector design. We began with a simple detector: The lead promoter could be placed in front of Green Fluorescent Protein, GFP. Once lead enters the cell, it activates the Lead Binding Protein, which activates our Lead Promoter, which in turn gives us some GFP output. But we realized that we could make this simple detector much more effective with an amplification circuit.
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</p><p><a href="/wiki/index.php/Image:Lead_Switch.jpg" class="image" title="Image:Lead Switch.jpg"><img src="/wiki/images/2/26/Lead_Switch.jpg" alt="Image:Lead Switch.jpg" width="241" height="181" longdesc="/wiki/index.php/Image:Lead_Switch.jpg" /></a>
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<div class="editsection" style="float:right;margin-left:5px;">[<a href="/wiki/index.php?title=Lead&amp;action=edit&amp;section=3" title="Lead">edit</a>]</div><a name="Experimental_Steps"></a><h1><a href="/wiki/index.php/%5CExperimental_Steps" title="\Experimental Steps"> Experimental Steps</a></h1>
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<p>We have three experimental steps to create our final product:
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1. Develop an assay to measure AHL
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2. Characterize our amplifier
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3. Characterize our new parts: the lead promoter and the lead binding protein
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<a href="/wiki/index.php/Image:Experiment.jpg" class="image" title="Image:Experiment.jpg"><img src="/wiki/images/d/d3/Experiment.jpg" alt="Image:Experiment.jpg" width="288" height="216" longdesc="/wiki/index.php/Image:Experiment.jpg" /></a>
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</p><p><strong>Parts added to the Registry:</strong>
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</p><p><a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=Brown" class='external text' title="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=Brown" rel="nofollow">Lead Detection Biobricks</a>
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</p><p><strong>Fully characterized 2 existing parts:</strong>
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</p><p><a href="http://partsregistry.org/Part:BBa_T9002:Experience" class='external text' title="http://partsregistry.org/Part:BBa T9002:Experience" rel="nofollow">AHL to GFP Converter (BBa_T9002)</a>
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</p><p><a href="http://partsregistry.org/Part:BBa_J37015:Experience" class='external text' title="http://partsregistry.org/Part:BBa J37015:Experience" rel="nofollow">Amplifier by Imperial College (BBa_J37105)</a>
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Revision as of 07:35, 15 July 2012

Team Colombia @ 2012 iGEM

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LizardoHello.png


<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> Lead - 2007.igem.org

Lead

From 2007.igem.org

Brown University
Home The Team Tristable Switch Community Supplemental About us


Click on the title to learn more about each section.


Lead Detection

According to the WHO, 40% of the planet does not have access to clean water. Lead is a serious contaminant around the world. It can cause neurological disorders, anemia, and death. In the United States alone, 3.5 million people have high levels of lead in their blood, so the problem is prevalent in both the developed and developing world. Children under the age of 12 are most at risk for lead poisoning. For more on lead poisoning, click here.

Image:Inspiration.jpg

Design

Having found a Lead Binding Protein and its corresponding Lead Promoter in the literature, we began to generate a number of ideas for our lead detector design. We began with a simple detector: The lead promoter could be placed in front of Green Fluorescent Protein, GFP. Once lead enters the cell, it activates the Lead Binding Protein, which activates our Lead Promoter, which in turn gives us some GFP output. But we realized that we could make this simple detector much more effective with an amplification circuit.

Image:Lead Switch.jpg


Experimental Steps

We have three experimental steps to create our final product: 1. Develop an assay to measure AHL 2. Characterize our amplifier 3. Characterize our new parts: the lead promoter and the lead binding protein Image:Experiment.jpg




Parts added to the Registry:

Lead Detection Biobricks

Fully characterized 2 existing parts:

AHL to GFP Converter (BBa_T9002)

Amplifier by Imperial College (BBa_J37105)