Team:Colombia

From 2012.igem.org

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(Replaced content with "= '''Team Colombia @ 2012 iGEM''' = {{https://2012.igem.org/User:Tabima}} center")
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<link rel="alternate" type="application/atom+xml" title="2011.igem.org Atom feed" href="/wiki/index.php?title=Special:RecentChanges&amp;feed=atom" /> <title>Team:HokkaidoU Japan - 2011.igem.org</title>
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    line-height: 1.2em;
+
-
}
+
-
h2{
+
-
    border-bottom: 1px dotted #e4e4e4;
+
-
}
+
-
ul, ol{
+
-
    margin: 1em 0 1em 0;
+
-
    padding: 0 0 0 1em;
+
-
}
+
-
pre{
+
-
    margin: 1em 0;
+
-
    padding: 1em;
+
-
    border: 1px solid #e4e4e4;
+
-
    background: #f7f7f7;
+
-
    -webkit-border-radius: 4px;
+
-
    -moz-border-radius: 4px;
+
-
    border-radius: 4px;
+
-
}
+
-
table{
+
-
    border-collapse: collapse;
+
-
    border: 1px solid #ababab;
+
-
    font-size: 100%;
+
-
}
+
-
table th, table td{
+
-
    padding: 0.4em 0.6em;
+
-
}
+
-
</style>
+
-
<style>
+
-
div.toc-wrapper {
+
-
  display: block;
+
-
  position: fixed;
+
-
  top: 20%;
+
-
  right: 0;
+
-
  width: 300px;
+
-
  height: 70%;
+
-
  z-index: 10000;
+
-
}
+
-
 
+
-
#toc {
+
-
  display: block;
+
-
  height: 100%;
+
-
  overflow: auto;
+
-
}
+
-
 
+
-
div.toc-tab {
+
-
  background: #dedede url(/wiki/images/f/f8/HokkaidoU_toc_tab.png) left top no-repeat;
+
-
  top: 20%;
+
-
  right: 0;
+
-
  width: 30px;
+
-
  height: 120px;
+
-
  display: block;
+
-
  position: fixed;
+
-
  z-index: 9999;
+
-
  cursor: pointer;
+
-
}
+
-
 
+
-
div.toc-wrapper div.close {
+
-
  background: url(/wiki/images/7/73/HokkaidoU_toc_close.png) left top no-repeat;
+
-
  top : 10px;
+
-
  right : 20px;
+
-
  width: 20px;
+
-
  height: 20px;
+
-
  display: block;
+
-
  position: absolute;
+
-
  cursor: pointer;
+
-
}
+
-
</style>
+
-
</p><p><style>
+
-
*:after{
+
-
    display: block;
+
-
    clear: both;
+
-
}
+
-
body{
+
-
    font-size: 76%;
+
-
    background: #ffffff;
+
-
}
+
-
#hokkaidou-container{
+
-
    width: 980px;
+
-
    margin: 0 auto;
+
-
    padding: 0;
+
-
}
+
-
#hokkaidou-header{
+
-
    background: #ffffff url(https://static.igem.org/mediawiki/2011/2/2b/Hokkaidou_header.png) repeat-x;
+
-
    margin-bottom: 10px;
+
-
}
+
-
#hokkaidou-footer{
+
-
    margin-top: 30px;
+
-
    padding-top: 15px;
+
-
    border-top: 1px solid #e4e4e4;
+
-
    text-align: center;
+
-
}
+
-
#hokkaidou-footer #hokkaidou-sponsors ul li{
+
-
    display: inline-block;
+
-
    margin: 10px;
+
-
    list-style: none;
+
-
}
+
-
#hokkaidou-footer #hokkaidou-sponsors ul:after {content: "";}
+
-
</style>
+
-
</p><p><style>
+
-
#hokkaidou-header{
+
-
    position: relative;
+
-
    height: 117px;
+
-
}
+
-
#hokkaidou-header #hokkaidou-logo h1 a{
+
-
    display: block;
+
-
    position: absolute;
+
-
    top: 10px; left: 20px;
+
-
    width: 400px;
+
-
    height: 75px;
+
-
    background: url(https://static.igem.org/mediawiki/2011/e/e5/Hokkaidou_logo.png) no-repeat;
+
-
    border: none;
+
-
    text-indent: -10000px;
+
-
    outline: none;
+
-
}
+
-
#hokkaidou-header #hokkaidou-logo h1 a:hover {
+
-
    background-position: 0 -76px;
+
-
}
+
-
#hokkaidou-header #hokkaidou-logo h1 a:active {
+
-
    background-position: 0 -152px;
+
-
}
+
-
#hokkaidou-header #hokkaidou-logo p{
+
-
    display: none !important;
+
-
}
+
-
</style>
+
-
</p><p><style>
+
-
#hokkaidou-header #hokkaidou-navigation ul{
+
-
    position: absolute;
+
-
    top: 10px; right: 0;
+
-
}
+
-
#hokkaidou-header #hokkaidou-navigation ul li{
+
-
    float: left;
+
-
    list-style: none;
+
-
    margin: 0;
+
-
    text-align: center;
+
-
    width: 60px;
+
-
}
+
-
#hokkaidou-header #hokkaidou-navigation ul li a,
+
-
#hokkaidou-header #hokkaidou-navigation ul li strong.selflink{
+
-
    display: inline-block;
+
-
    width: 30px; height: 30px;
+
-
    background: url(https://static.igem.org/mediawiki/2011/4/4b/Hokkaidou_menu.png) no-repeat;
+
-
    border: none;
+
-
    outline: none;
+
-
    text-indent: -10000px;
+
-
}
+
-
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-home a{ background-position: 0 0; }
+
-
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-home a:hover { background-position: -30px 0; }
+
-
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-home a:active,
+
-
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-home strong.selflink{ background-position: -60px 0; }
+
-
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-project a{ background-position: 0 -30px; }
+
-
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-project a:hover { background-position: -30px -30px; }
+
-
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-project a:active,
+
-
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-project strong.selflink{ background-position: -60px -30px; }
+
-
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-protocol a{ background-position: 0 -60px; }
+
-
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-protocol a:hover { background-position: -30px -60px; }
+
-
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-protocol a:active,
+
-
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-protocol strong.selflink{ background-position: -60px -60px; }
+
-
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-parts a{ background-position: 0 -90px; }
+
-
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-parts a:hover { background-position: -30px -90px; }
+
-
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-parts a:active,
+
-
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-parts strong.selflink{ background-position: -60px -90px; }
+
-
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-notebook a{ background-position: 0 -120px; }
+
-
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-notebook a:hover { background-position: -30px -120px; }
+
-
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-notebook a:active,
+
-
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-notebook strong.selflink{ background-position: -60px -120px; }
+
-
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-safety a{ background-position: 0 -150px; }
+
-
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-safety a:hover { background-position: -30px -150px; }
+
-
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-safety a:active,
+
-
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-safety strong.selflink{ background-position: -60px -150px; }
+
-
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-humanpractice a{ background-position: 0 -180px; }
+
-
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-humanpractice a:hover { background-position: -30px -180px; }
+
-
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-humanpractice a:active,
+
-
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-humanpractice strong.selflink{ background-position: -60px -180px; }
+
-
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-team a{ background-position: 0 -210px; }
+
-
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-team a:hover { background-position: -30px -210px; }
+
-
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-team a:active,
+
-
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-team strong.selflink{ background-position: -60px -210px; }
+
-
 
+
-
/* i couldn't be bothered to add text on picture... lol */
+
-
#hokkaidou-header #hokkaidou-navigation ul li:after {
+
-
    display: inline-block;
+
-
    font-family: 'Josefin Sans', sans-serif;
+
-
    font-size: 12px;
+
-
    margin-top: 5px;
+
-
    width: 100%;
+
-
    line-height: 14px;
+
-
}
+
-
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-home:after {content: "Home";}
+
-
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-project:after {content: "Project";}
+
-
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-protocol:after {content: "Protocol";}
+
-
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-parts:after {content: "Parts";}
+
-
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-notebook:after {content: "Notebook";}
+
-
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-safety:after {content: "Safety";}
+
-
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-humanpractice:after {content: "Human Practice";}
+
-
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-team:after {content: "Team";}
+
-
</style>
+
-
</p><p><style>
+
-
#hokkaidou-content{zoom: 1;}
+
-
#hokkaidou-content:after { content: ""; }
+
-
#hokkaidou-content #hokkaidou-left-content{
+
-
    float: left;
+
-
    width: 280px;
+
-
    margin-right: 20px;
+
-
}
+
-
#hokkaidou-content #hokkaidou-left-content ul{
+
-
    margin: 0;
+
-
    padding: 0 1em;
+
-
}
+
-
#hokkaidou-content #hokkaidou-left-content ul li{
+
-
    list-style: none;
+
-
    margin: 0 0 1em 0;
+
-
}
+
-
#hokkaidou-content #hokkaidou-left-content ul li .hokkaidou-description{
+
-
    font-size: small;
+
-
    color: #8a8a8a;
+
-
}
+
-
#hokkaidou-content #hokkaidou-right-content{
+
-
    float: right;
+
-
    width: 680px;
+
-
}
+
-
</style>
+
-
</p><p><style>
+
-
#hokkaidou-index-menu{
+
-
    margin: 0 0 30px 0 !important;
+
-
    padding: 0 !important;
+
-
    border: 1px solid #ababab;
+
-
    -webkit-border-top-right-radius: 12px;
+
-
    -moz-border-radius-topright: 12px;
+
-
    border-top-right-radius: 12px;
+
-
    -webkit-border-bottom-right-radius: 12px;
+
-
    -moz-border-radius-bottomright: 12px;
+
-
    border-bottom-right-radius: 12px;
+
-
}
+
-
#hokkaidou-index-menu li{
+
-
    list-style: none;
+
-
    margin: 0 !important;
+
-
    padding: 0 !important;
+
-
    background: -moz-radial-gradient(center 80px 45deg, circle cover, hsl(0, 0%, 100%) 0%, hsl(240, 5%, 70%) 100%);
+
-
    overflow: hidden;
+
-
    text-shadow: 0 1px 1px hsl(240, 10%, 80%);
+
-
}
+
-
#hokkaidou-index-menu li:first-child{
+
-
    -webkit-border-top-right-radius: 12px;
+
-
    -moz-border-radius-topright: 12px;
+
-
    border-top-right-radius: 12px;
+
-
}
+
-
#hokkaidou-index-menu li:last-child{
+
-
    -webkit-border-bottom-right-radius: 12px;
+
-
    -moz-border-radius-bottomright: 12px;
+
-
    border-bottom-right-radius: 12px;
+
-
}
+
-
 
+
-
#hokkaidou-index-menu li a{
+
-
    display: block;
+
-
    padding: 10px 20px;
+
-
    border-bottom: none;
+
-
    color: #3b3b3b;
+
-
    transition: background 0.5s ease-in-out;
+
-
    -moz-transition-property: background;
+
-
    -moz-transition-duration: 700ms;
+
-
    background: #e4e4e4;
+
-
    border-bottom: 1px solid #dedede;
+
-
    border-top: 1px solid #f7f7f7;
+
-
}
+
-
#hokkaidou-index-menu li a{
+
-
}
+
-
#hokkaidou-index-menu li a:hover{
+
-
    background: transparent none;
+
-
}
+
-
#hokkaidou-index-menu li a .hokkaidou-description{
+
-
    display: block;
+
-
    margin-left: 40px;
+
-
}
+
-
#hokkaidou-index-menu li a:before{
+
-
    display: block;
+
-
    float: left;
+
-
    content: "";
+
-
    width: 30px; height: 30px;
+
-
    margin: 5px 10px 0 0;
+
-
    background: url(https://static.igem.org/mediawiki/2011/4/4b/Hokkaidou_menu.png) no-repeat;
+
-
}             
+
-
#hokkaidou-index-menu li#hokkaidou-index-project a:before{ background-position: 0 -30px; }
+
-
#hokkaidou-index-menu li#hokkaidou-index-protocol a:before{ background-position: 0 -60px; }
+
-
#hokkaidou-index-menu li#hokkaidou-index-parts a:before{ background-position: 0 -90px; }
+
-
#hokkaidou-index-menu li#hokkaidou-index-notebook a:before{ background-position: 0 -120px; }
+
-
#hokkaidou-index-menu li#hokkaidou-index-safety a:before{ background-position: 0 -150px; }
+
-
#hokkaidou-index-menu li#hokkaidou-index-humanpractice a:before{ background-position: 0 -180px; }
+
-
#hokkaidou-index-menu li#hokkaidou-index-team a:before{ background-position: 0 -210px; }
+
-
 
+
-
#hokkaidou-index-menu li#hokkaidou-index-project a:hover:before{ background-position: -30px -30px; }
+
-
#hokkaidou-index-menu li#hokkaidou-index-protocol a:hover:before{ background-position: -30px -60px; }
+
-
#hokkaidou-index-menu li#hokkaidou-index-parts a:hover:before{ background-position: -30px -90px; }
+
-
#hokkaidou-index-menu li#hokkaidou-index-notebook a:hover:before{ background-position: -30px -120px; }
+
-
#hokkaidou-index-menu li#hokkaidou-index-safety a:hover:before{ background-position: -30px -150px; }
+
-
#hokkaidou-index-menu li#hokkaidou-index-humanpractice a:hover:before{ background-position: -30px -180px; }
+
-
#hokkaidou-index-menu li#hokkaidou-index-team a:hover:before{ background-position: -30px -210px; }
+
-
 
+
-
#hokkaidou-official-team-logo{
+
-
    text-align: center;
+
-
}
+
-
#hokkaidou-official-team-logo img{
+
-
    display: block;
+
-
    width: 240px;
+
-
    height: 240px;
+
-
    border: 1px solid #e4e4e4;
+
-
    margin: 0 auto;
+
-
}
+
-
#hokkaidou-official-team-logo .hokkaidou-description{
+
-
    font-size: small;
+
-
    color: #ababab;
+
-
}
+
-
#hokkaidou-links ul{
+
-
    margin: 20px 0 !important;
+
-
    padding: 0 !important;
+
-
}
+
-
#hokkaidou-links ul {zoom: 1;}
+
-
#hokkaidou-links ul:after {content: ""; }
+
-
#hokkaidou-links ul li{
+
-
    float: left;
+
-
    display: block !important;
+
-
    margin: 0 5px !important;
+
-
    padding: 0 !important;
+
-
}
+
-
#hokkaidou-links ul li:first-child{
+
-
    margin-left: 0 !important;
+
-
}
+
-
#hokkaidou-links ul li a{
+
-
    display: block;
+
-
    width: 32px;
+
-
    height: 32px;
+
-
    background: url(https://static.igem.org/mediawiki/2011/8/8b/IGEM_HokkaidoU_Index_Links.png) no-repeat;
+
-
    text-indent: -10000px;
+
-
}
+
-
#hokkaidou-links ul li#hokkaidou-links-email a{ background-position: 0 0; }
+
-
#hokkaidou-links ul li#hokkaidou-links-facebook a{ background-position: -32px 0; }
+
-
#hokkaidou-links ul li#hokkaidou-links-twitter a{ background-position: -64px 0; }
+
-
#hokkaidou-links ul li#hokkaidou-links-wordpress a{ background-position: -96px 0; }
+
-
#hokkaidou-links ul li#hokkaidou-links-youtube a{ background-position: -128px 0; }
+
-
#hokkaidou-links ul li#hokkaidou-links-email{
+
-
    display: block !important;
+
-
    width: 32px !important;
+
-
    height: 32px !important;
+
-
    margin: 0 5px 0 0 !important;
+
-
    padding: 0 !important;
+
-
}
+
-
#hokkaidou-links ul li#hokkaidou-links-email a{
+
-
    display: block !important;
+
-
    width: 100% !important;
+
-
    height: 100% !important;
+
-
    margin: 0 !important;
+
-
    padding: 0 !important;
+
-
    background: url(https://static.igem.org/mediawiki/2011/8/8b/IGEM_HokkaidoU_Index_Links.png) no-repeat !important;
+
-
    text-indent: -10000px !important;
+
-
}
+
-
</style>
+
-
 
+
-
<script type="text/javascript">
+
-
jQuery.cookie=function(d,f,b){function e(j,i){if(j){if(i){return true}}return false}if(e(arguments.length>1,String(f)!=="[object Object]")){b=jQuery.extend({},b);if(f===null||f===undefined){b.expires=-1}if(typeof b.expires==="number"){var h=b.expires,c=b.expires=new Date();c.setDate(c.getDate()+h)}f=String(f);return(document.cookie=[encodeURIComponent(d),"=",b.raw?f:encodeURIComponent(f),b.expires?"; expires="+b.expires.toUTCString():"",b.path?"; path="+b.path:"",b.domain?"; domain="+b.domain:"",b.secure?"; secure":""].join(""))}b=f||{};var a,g=b.raw?function(i){return i}:decodeURIComponent;return(a=new RegExp("(?:^|; )"+encodeURIComponent(d)+"=([^;]*)").exec(document.cookie))?g(a[1]):null};
+
-
</script>
+
-
<script type="text/javascript">
+
-
$('.right-menu, .left-menu').fadeTo(0, 0);
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            <h1> <span class="mw-headline" id="HokkaidoU_Japan"><a href="https://2011.igem.org/Team:HokkaidoU_Japan">HokkaidoU Japan</a></span></h1>
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            <p class="description">iGEM 2011 Team of Hokkaido University</p>
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            <li id="hokkaidou-menu-home"><strong class="selflink">Home</strong></li>
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            <li id="hokkaidou-menu-protocol"><a href="/Team:HokkaidoU_Japan/Protocols" title="Team:HokkaidoU Japan/Protocols">Protocols</a></li>
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            <li id="hokkaidou-menu-humanpractice"><a href="/Team:HokkaidoU_Japan/HumanPractice" title="Team:HokkaidoU Japan/HumanPractice">Human Practice</a></li>
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    <table id="toc" class="toc"><tr><td><div id="toctitle"><h2>Contents</h2></div>
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<ul>
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<li class="toclevel-1"><a href="#HokkaidoU_Japan"><span class="tocnumber">1</span> <span class="toctext">HokkaidoU Japan</span></a>
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<ul>
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<li class="toclevel-2 tocsection-1"><a href="#Acknowledgements"><span class="tocnumber">1.1</span> <span class="toctext">Acknowledgements</span></a>
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<ul>
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<li class="toclevel-3 tocsection-2"><a href="#Laboratory_and_Personnel"><span class="tocnumber">1.1.1</span> <span class="toctext">Laboratory and Personnel</span></a></li>
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<li class="toclevel-3 tocsection-3"><a href="#Academic_Institutions"><span class="tocnumber">1.1.2</span> <span class="toctext">Academic Institutions</span></a></li>
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<li class="toclevel-3 tocsection-4"><a href="#Companies"><span class="tocnumber">1.1.3</span> <span class="toctext">Companies</span></a></li>
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        <li id="hokkaidou-index-team"><a href="https://2011.igem.org/Team:HokkaidoU_Japan/Team">Team
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            <span class="hokkaidou-description">
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                The HokkaidoU_Japan is formed by 16 students and 3 instructors
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            </span>
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        </a>
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        <li id="hokkaidou-index-project"><a href="https://2011.igem.org/Team:HokkaidoU_Japan/Project">Project
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                We tried to further develop E. coli-based protein injection system which we started working on during iGEM 2010
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        <li id="hokkaidou-index-parts"><a href="https://2011.igem.org/Team:HokkaidoU_Japan/Parts">Parts
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                Ready-to-inject backbone and Bsa I cloning system
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            </span>
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        </a>
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        </li>
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        <li id="hokkaidou-index-protocol"><a href="https://2011.igem.org/Team:HokkaidoU_Japan/Protocols">Protocol
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                General protocols, Primers and methods of infection assay
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            </span>
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        </a>
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        <li id="hokkaidou-index-notebook"><a href="https://2011.igem.org/Team:HokkaidoU_Japan/Notebook">Notebook
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                The record of our many failures and a bit of achievement
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                Addressing safety concerns
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                Science art gallery "BIO ART"
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                    <div class="lof-main-item-desc">
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                        <h2>HokkaidoU_Japan 2011</h2>
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                    </div>
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                </li>
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                <li>
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                    <img src="https://static.igem.org/mediawiki/2011/8/8a/IGEM_HokkaidoU_Index_Slideshow_1.jpg">         
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                    <div class="lof-main-item-desc">
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                        <h2>Welcome to the BIOxART Gallery!!</h2>
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                </li>
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                    <div class="lof-main-item-desc">
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                        <h2>Be careful not to contaminate...</h2>
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                        <h2>Meeting, meeting and MEETING!</h2>
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                        <h2>Dr. <i>E. coli</i> get ready for the job</h2>
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                    <img src="https://static.igem.org/mediawiki/2011/0/06/IGEM_HokkaidoU_Index_Slideshow_5.jpg">           
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                    <div class="lof-main-item-desc">
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                        <h2>BIOxART, please feel free to look around</h2>
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                    <div class="lof-main-item-desc">
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                        <h2>Experiment, experiment and EXPERIMENT!</h2>
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                </li>
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            </ul>   
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                    <li class="" style="height: 32px; width: 80px;"><img src="https://static.igem.org/mediawiki/2011/8/81/IGEM_HokkaidoU_Index_Slideshow_Thumb_7.jpg"></li>
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                    <li class="" style="height: 32px; width: 80px;"><img src="https://static.igem.org/mediawiki/2011/f/f3/IGEM_HokkaidoU_Index_Slideshow_Thumb_1.jpg"></li>
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                    <li class="" style="height: 32px; width: 80px;"><img src="https://static.igem.org/mediawiki/2011/a/aa/IGEM_HokkaidoU_Index_Slideshow_Thumb_2.jpg"></li>
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                    <li class="" style="height: 32px; width: 80px;"><img src="https://static.igem.org/mediawiki/2011/f/fb/IGEM_HokkaidoU_Index_Slideshow_Thumb_3.jpg"></li>
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                    <li class="" style="height: 32px; width: 80px;"><img src="https://static.igem.org/mediawiki/2011/e/e2/IGEM_HokkaidoU_Index_Slideshow_Thumb_4.jpg"></li>
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                    <li class="" style="height: 32px; width: 80px;"><img src="https://static.igem.org/mediawiki/2011/9/93/IGEM_HokkaidoU_Index_Slideshow_Thumb_5.jpg"></li>
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                    <li class="" style="height: 32px; width: 80px;"><img src="https://static.igem.org/mediawiki/2011/2/21/IGEM_HokkaidoU_Index_Slideshow_Thumb_6.jpg"></li>
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            </div>
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        </div>
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    </div>
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    <h2>Abstract</h2>
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    <p>We further developed "Dr. E. coli": our project of iGEM 2010. Last year, we showed that Type 3 Secretion System (T3SS) works in E. coli by injecting GFP into RK13 cells. T3SS is a syringe like organelle found in bacterium such as Salmonella which uses it to inject virulence effector proteins into a target eukaryotic cell. We think this system can be applied to direct reprogramming of somatic cells among many other things.</p>
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    <p>We tested T3SS performance and tried to make it more convenient. For this purpose we designed a plasmid backbone which can instantly produce ready-to-inject fusion proteins from ordinary biobrick part. Using it, we tried to further characterize this system by injecting a library of protein domains.</p>
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    <p>In Science Gallery we exhibited awesome photographs related with biotechnology in public. We tried to catch the pedestrians’ interest in current synthetic biology and explore their thoughts.</p>
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    <h2>BioArt</h2>
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    <iframe id="hokkaidou-bioart-youtube" width="680" height="360" src="http://www.youtube.com/embed/nOP0YIUSj-s" frameborder="0" allowfullscreen></iframe>
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    <p>We held the new type science art gallery "Bio Art" at the center of Sapporo city.</p>
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    <p>This gallery is not only for our team.You can also enjoy Bio Art on this Web page.
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    You can watch more detail in <a href="https://2011.igem.org/Team:HokkaidoU_Japan/HumanPractice">Human Practice</a> page.</p>
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</p>
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<h2> <span class="mw-headline" id="Acknowledgements">Acknowledgements</span></h2>
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<h3> <span class="mw-headline" id="Laboratory_and_Personnel">Laboratory and Personnel</span></h3>
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<ul><li> Environmental Molecular Biology, Graduate School of Environmental Earth Science, Hokkaido University
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</li></ul>
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<dl><dd><dl><dd> Ken-ichi Yamazaki and Members of Yamazaki Lab
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</dd></dl>
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</dd></dl>
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<ul><li> Molecular Biology, Institute for Genetic Medicine, Hokkaido University
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</li></ul>
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<dl><dd><dl><dd> Hisatoshi Shida
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</dd></dl>
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</dd></dl>
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<ul><li> Nikon Imaging Center
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</li></ul>
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<dl><dd><dl><dd> Takeharu Nagai, Kenta Saito, Masahiro Nakano, Kentarou Kobayashi, Hiroshi Nishino
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</dd></dl>
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</dd></dl>
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<ul><li> Laboratory of Molecular and Cellular Biophysics, Hokkaido University
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</li></ul>
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<dl><dd><dl><dd> Tomomi Nemoto, Terumasa Hibi, Ryosuke Kawakami
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</dd></dl>
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</dd></dl>
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<ul><li> Muroran Marine Station Field Science Center for Northern Biosphere, Hokkaido University
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</li></ul>
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<dl><dd><dl><dd> Taizo Motomura
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</dd></dl>
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</dd></dl>
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<ul><li> Laboratory of X-ray structural biology, Department of Advanced Transdisciplinary Science, faculty of Advanced Life Science
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</li></ul>
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<dl><dd><dl><dd>Isao Tanaka
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</dd></dl>
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</dd></dl>
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<ul><li> Research Institute for Electronic Science, Hokkaido University
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</li></ul>
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<h3> <span class="mw-headline" id="Academic_Institutions">Academic Institutions</span></h3>
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<ul><li> Salmonella Genetic Stock Center/University of Calgary
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</li><li> Graduate School of Environmental Earth Science
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</li><li> Department of biological science/School of Sciense
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</li><li> Institute for Genetic Medicine
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</li></ul>
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<h3> <span class="mw-headline" id="Companies">Companies</span></h3>
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<ul><li> Primary Cell Co., Ltd.
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</li><li> ECONiXE Co., Ltd.
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</li><li> Hokuto Kohki Engineering Co,. Ltd.
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</li><li> COSMO BIO Co., Ltd.
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</li><li> AMINO UP CHEMICAL Co., Ltd.
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</li><li> Mendel Workshop Co., Ltd.
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</li></ul>
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                <li><a href="http://www.cosmobio.co.jp/index_e.asp" target="_blank">
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                    <img src="https://static.igem.org/mediawiki/2011/4/44/IGEM_HokkaidoU_sponsorlogo_cosmobio.png" alt="COSMO BIO CO., LTD." />
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                    <img src="https://static.igem.org/mediawiki/2011/7/7c/IGEM_HokkaidoU_sponsorlogo_aminoup.png" alt="株式会社 アミノアップ化学" />
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Revision as of 08:05, 15 July 2012

Team Colombia @ 2012 iGEM

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